Genetic Interactions Between Tumor Suppressors PTEN and TSC2 in Tumor Suppression in the Mouse

Supplementary data

Supplementary Figure 1. Reduction of the Tsc2 dose leads to exacerbated symptoms accompanied with lymphoadenopathy and splenomegaly in Pten+/- mice.

(A) B220 and CD3 staining of hyperplastic Pten+/- and Pten+/-Tsc2+/- lymph nodes on consecutive sections show indistinguishable patterns between the two different mutants (100x).

(B) Acute and chronic inflammation in the liver of a 6-month-old Pten+/-Tsc2+/- mouse. Arrows indicate polymorphonuclear cells (600x).

(C) H&E stained sections show exacerbated lymphoid infiltration in the kidney

(40x) and liver (100x) of a 6-month-old Pten+/-Tsc2+/- mouse compared with its

Pten+/- and Tsc2+/- littermates.

(D) H&E sections reveal severe extramedullary hematopoiesis in the spleen of a

6-month-old Pten+/-Tsc2+/- mouse compared with its littermates (40x). Note the normal spleen histology and well-defined white and red pulp in the spleen of the

Tsc2+/- mouse, while only mild red pulp expansion with mild focal extramedullary hematopoiesis is observed in the spleen of the Pten+/- mouse.

(E) Pelvic abscess with a necrotic center in a 6-month-old Pten+/-Tsc2+/- female mouse. The right panel (a magnified image of the boxed region in the left panel,

20x) represents the surrounding neutrophils, foamy histiocytes, and a fibrotic wall

(200x). Supplementary Figure 2. High-grade PIN and invasive carcinoma in AP, VP and DLP of an 8-month-old Pten+/-Tsc2+/- mouse, and in AP of a 5-month-old

Pten+/-Tsc2+/- mouse. Boxed regions and arrows indicate the area of invasion

(100x; 400x).