Anis Ahmad Chaudhary

CURRICULUM VITAE Dr. Anis Ahmad Chaudhary

ACADEMIC QUALIFICATION  Doctor of Philosophy (Ph. D.)(2012) Faculty of Science, Jamia Hamdard (Hamdard University) New Delhi-110062, India Topic: “Development of LAMP-based Molecular markers for authentication of some medicinal plants”  M.Sc. Biotechnology (2005) Deptt. of Bioscience, Jamia Millia Islamia (A Central University) New Delhi-110025, India  B.Sc. Biology (2001) CCRD College, CCS University Meerut-251002, India PRESENT STATUS Assistant Professor (22nd Oct. 2013 to Till Date) College of Medicine Al-Imam Muhammad Ibn Saud Islamic University Riyadh- 13317-4233 Kingdom of Saudi Arabia PAST EXPERIENCE 1. Academic Experience a. DST Young Scientist (Govt. of India), (One year 6th Aug 2012 to 15th Aug 2013) Deptt. of Bioscience Faculty of Natural Science, Jamia Millia Islamia New Delhi-110025, India Topic: “Molecular characterization of Virulence gene and Silver nano particles activity evaluation against Diarrhoeagenic Escherichia coli” b. Senior Research Fellow (SRF), (One and half years 20th April 2011 to 5th Aug 2012) Deptt. of Ilmul Advia (Pharmacology) Faculty of Medicine, Jamia Hamdard (Hamdard University) New Delhi-110062, India Topic: “Application and cost-effectiveness of LAMP (loop-mediated isothermal amplification)-based technology for authentication of medicinal plants” c. Junior Research Fellow (JRF) (One and half years 20th Oct 2008 to 3rd Jun 2010) Faculty of Science, Jamia Hamdard (Hamdard University) New Delhi-110062, India Topic: “Proteome analysis of nitrogen-efficient and nitrogen-inefficient rice genotypes” 2. Industrial Experience I. Manager (Technical Support) (3 years, 15th April 2008 to 8 Feb 2011) 1 Vision Diagnostics (India) Pvt. Ltd. New Delhi-110092, India Responsibilities Standardization and Demonstration of diagnostic tests on PCR/RT-PCR, Real Time PCR (BioRed, Roche LC480, Cobas Aplicor, ABI 9600, Cephid, Roter Gene), HLA Genotyping (Molecular and Serological), Sequence Base HLA typing, Quantification of BCR/ABL gene by Real Time PCR, DNA/RNA Isolation to difference principle, DNA/RNA Hybridization, ELISA and Immunohistochemistry. II. Scientist and Training Coordinator, (7 months, 12th Sep 2007 to 14th April 2008) Atharva Biosciences Pvt. Ltd. New Delhi-110029, India Responsibilities  Standardization of molecular diagnostic assay & developments in house protocols on PCR/RT-PCR, Real Time PCR and LAMP Assay.  Undertaking contract research.  Training Coordinator and Management Representative. 3. Training I. Industrial Trainee Biotech Consortium India Limited (six months 3rd Feb 2007 to 2nd Sep2007) Auroprobe Laboratories (Sponsored by DBT, Govt. of India) New Delhi-110029, India Topic: “RNA Isolation HCV Selection Using COBAS AMPLICOR, Herpis Simplex Virus, PCR Reaction Mixture for MTb detection” II. Project Training (one Year 14th Jan 2005 to 25th Dec 2005) Hepatitis Lab Maulana Azad Medical College (Govt. of India) New Delhi-110001, India Topic: “Diagnostic utility of hepatitis C virus core antigen in hemodialysis patients” PROFESSIONAL EXPERTISE  Ion Torrent DNA Sequencer (Next Generation Sequencing).  ABI 16 capillaries DNA Sequencer set up.  Standardization of molecular diagnostic assay & development in house protocols for Real Time PCR/PCR (BioRed, Roche, ABI, Cephid, Roter Gene), Multiplex PCR, RAPD and AFLP.  Loop Mediated Isothermal Amplification (LAMP) a novel, specific and rapid technique for amplification of DNA under isothermal conditions.  DNA/RNA Hybridization for HCV Genotyping.  HLA Genotyping (Molecular and Serological), HLA Sequence Base Typing.  Quantification & Detection of BCR\ABL gene with PCR, Real Time PCR.  cDNA preparation of mRNA and genome of RNA virus.  DNA/RNA isolation from whole blood, tissue, cell culture and different Bacteria’s, Viruses by using Column Base and Trizol method. 2  Expertise in Animal Handling as surgery, evaluation of maximum tolerated dose in mice and dosing through various routes and biochemical parameters.  Decontamination and Culture of Mycobacterium Tuberculosis in Fast Plug Assay and BACTEC 460 TB Systems, Gram & ZN staining.  Restriction-Digestion, Gel electrophoresis, ELISA, IHC, Phytochemical analysis and Standardization of TLC, HPTLC. BIO-INFORMATICS: Primer Designing, FASTA, BLAST, CLUSTAL-W, OMIM, Primer3, LAMP-primer explorer V3. FIELDS OF INTEREST Nano Technology, Molecule Biology, Cell Biology, Virology PUBLICATIONS Research Papers Published/Accepted-23 Communicated-03 Under Preparation-02 Book Chapter-01 Conference/Seminars-05 Abstract Published-08

MEMBER OF EDITORIAL BOARD OF JOURNALS

I. JSM Internal Medicine http://www.jscimedcentral.com/InternalMedicine/editors.php II. SciTz Diabetes and Metabolism http://www.scitechz.com/Diabetes_Editorialboard.html III. Austin Diabetes Research http://austinpublishinggroup.com/diabetes-research/editorialBoard.php IV. Journal of Metabolic Investigation http://www.smartscitech.com/index.php/JMI/about/editorialTeam

LIST OF PUBLICATIONS

1. Malik a, Kumar d, Khan AA, Khan AA, Chaudhary AA, Husain SA, Kar P (2016) Hepatitis B virus precore G1896A mutation in chronic liver disease patients with HBeAg negative serology from North India. Saudi Journal of Biological Sciences xxx, xxx–xxx 2. Alshehri SM, Lohedan HA, ChaudharyAA, Farraj E, Hokbany N, Issa Z, Housine S, Ahamad T. Delivery of ibuprofen by natural macroporous sporopollenin exine capsules extracted from Phoenix dactylifera L. European Journal of Pharmaceutical Sciences xxx (2016) xxx–xxx. 3. Alshehri SM, Lohedan HA, Farraj E, Hokbany N, Chaudhary AA, Ahamad T (2016) Macroporous natural capsules extracted from Phoenix dactylifera L. spore and their application in oral drugs delivery. International Journal of Pharmaceutics 504 :39–47.

3 4. Alshehri SM, Aldalbahi A, Al-hajji AB, Chaudhary AA, Panhuis M, Alhokbany N, Ahamad T (2015) Development of carboxymethyl cellulose-based hydrogel and nanosilver composite as antimicrobial agents for UTI pathogens. Carbohydrate Polymers 138 (2016) 229:236. 5. Shaqha WM, Khan M, Salam N, Azzi A, Chaudhary AA (2015) Anti-diabetic potential of Catharanthus roseus Linn. and its effect on the glucose transport gene (GLUT-2 and GLUT-4) in streptozotocin induced diabetic wistar rats. BMC Complementary and Alternative Medicine 15:379. 6. Shaqha WM, Fareed M, Chaudhary AA, Azzi A, Shaqha MW, Salam N (2016) Immunopathogenesis and epidemiology of Middle East Respiratory Syndrome Coronavirus. Journal of Applied Medical Sciences 5(1):53-64. 7. Shaqha WM, Khan M, Azzi A, Chaudhary AA (2015) Application and Cost-Effectiveness of LAMP (Loop-Mediated Isothermal Amplification) technology for authentication of medicinal plants Podopghyllum Hexandrum Royle. International Journal of Pharmaceutical Science and Research 6(5): 2035-2041. 8. Shaqha WM, Khan M, Chaudhary AA (2014) SCAR Molecular Markers for identification and authentication of medicinal plants Podophyllum Hexandrum Royle. Asian Journal of Biochemical and Pharmaceutical Research 4 (4): 66-75. 9. Shaqha WM, Janabi FA, Chaudhary AA, Kharfy KM (2014) Insulin prescribing practices in Saudi Arabia. World Journal of Pharmacy and Pharmaceutical Sciences 4 (2): 29-40. 10. Chaudhary AA, Khan M, Shaqha WM, Alharbi M, Khamees OA (2014). Rapid and easy molecular authentication of medicinal plant Zingiber officinale Roscoe by loop-mediated isothermal amplification (LAMP)-based marker. Journal of Medicinal Plants Research. 8(24): 756-762. 11. Chaudhary AA, Khan M, Ansari S, Chauhan V (2014). Phytochemical screening and antibacterial efficacy of Ficus benghalensis using in vitro models. International Journal of Pharmaceutical Sciences Review and Research 24(2): 276-279. 12. Chaudhary AA, Chauhan V, Ansari S, Khan M (2014). In vitro antimicrobial potential of coriandrum sativumagaist pathogenic microorganisms. International Journal of Advanced Research 2(1):208-211. 13. Abraham G, Pandey N, Mishra V, Chaudhary AA, Ahmad A, Singh R, Singh PK (2013). Development of SCAR based molecular marker for identification of different species of Azolla. India Journal of Biotechnology 12:489- 492 14. Ashraf K, Ahmad A, Chaudhary AA, Mujeeb M, Ahmad S, Amir M, Mallick N(2013).Genetic diversity analysis of Zingiber Officinale Roscoe by

4 RAPD collected from subcontinent of India. Saudi Journal of Biological Sciences doi.org/10.1016/j.sjbs.2013.09.005 15. Chaudhary AA, Yadav D, Hemant, Jamil SS, Asif M. (2012). Real Time Sequence Characterized Amplified Region (RT-SCAR) marker -Development and its application for authentication and quantification of Catharanthus roseus L. Don. Journal of Medicinal Plants Research. 6(32) 4748-4754. 16. Yadav D, Chaudhary AA, Garg V, Anwar MF, Rahman MM, Jamil SS, Khan HA, Asif M. (2012) In vitro toxicity and antidiabetic activity of a newly developed polyherbal formulation (MAC-ST/001) in streptozotocin-induced diabetic Wistar rats. Protoplasma DOI 10.1007/s00709-012-0458-7 17. Chaudhary AA, Dhar U, Ahmad A, Bhatt ID, Jugran A, Kaur G. (2012). Analysis of genetic diversity in Hedychium spicatum Buch. Ham. Ex. D. Don in Himalaya, India. Journal of Medicinal Plants Research. 6(23): 3984-3991. 18. Choudhary AA, Hemant, Khan M, Ahmad A (2011). Application of LAMP (Loop Mediated Isothermal Amplification)-based technology for authentication of Catharanthus roseus L. Don. Protoplasma 249: 417–422. 19. Yadav A, Ahmad J, Chaudhary AA, Ahmad A. (2012). Development of Sequence Characterized Amplification Region (SCAR) Marker for Authentication of Bacopa monnieri (L.) Wettst. European Journal of Medicinal Plants. 2(3): 186-198. 20. Wadhwa A, Iqbal M, Chaudhary AA, Ahmad A (2012). Role of Sulphur Metabolites in the regulation of uptake of Sulphate in Arabidopsis thaliana. International Journal of Agriculture Science. 3(2): 197-204. 21. Yadav D, Suri S, Choudhary AA, Sikender M, Hemant, Nadeem M, Garg V, Ahmad A, Asif M (2011). A Novel Approach: Herbal Remedies and Natural Products in Pharmaceutical Science as Nano Drug Delivery Systems. International Journal of Pharmacy and Technology 3: 3092-3116. 22. Yadav D, Suri S, Chaudhary AA, Hemant, Beg NM, Garg V, Asif M, Ahmad A (2012). Stimuli Responsive Polymeric Nanoparticles in Regulated Drug Delivery for Cancer. Polish Journal of Chemical technology. 14(1): 57-64. 23. Medhi S, Potukuchi SK, Polipalli SK, Swargiary SS, Deka P, Choudhary A, Begum N, Hussain Z, Ahlawat RS, Kar P (2008). Diagnostic utility of hepatitis C virus core antigen in hemodialysis patients. J Clin Biochem 41(7-8): 447-52. BOOK CHAPTER

5 1. Chaudhary AA, Ahmad A, Iqbal M (2012). Molecular Biology techniques for authentication of medicinal plants. Recent Trends in Medicinal Botany, (16): 295- 329. INTERNATIONAL CONFERENCE/WORKSHOP ATTENDED 1. Presented poster in International conference on "Recent Advances in Cardiovascular Sciences and Translational Medicine” RACS-5 &TM 2012, February 16, 2012, Amity University, Noida India. 2. International Nitrogen Conference held in Ashoka Hotel, New Delhi, India from 2-7 Dec 2010. 3. POLAR SCIENCE AND TECHNOLOGY held in Jamia Hamdard, New Delhi, India from 4-6 Oct 2009. 4. Workshop on VITRIFICATION held in Army Hospital, New Delhi, India from 20- 22 Feb 2009. 5. Workshop on ABI Sequencer system for High Resolution HLA typing with DNA sequencing by inno-train Diagnostic, Germany in held AIIMS, New Delhi, India from 1 July 2008 to 5 July 2008.

REFEREES

Place: New Delhi, India (Anis Ahmad Chaudhary)