EST Cdna Array

EST cDNA Array

The table below shows the arrangement of spots on the macroarrays. Each spot (except the controls - actin, GAPDH, EIF and neg) are give a number (for example, Gmm-1694 is 1, Gmm-0596 is 3). These numbers are used to identify each spot on the Excel spreadsheets on which the data are presented (lehanemacroarraydata.xls). Background and negative control values have already been subtracted from each experimental value. Any negative values resulting were set to zero. For normalization of total intensity on each array the mean of the internal control spots (GAPDH, actin, EIF) was averaged for each membrane separately. Values for the other spots on the membrane were then expressed relative to this average for the internal control spots. These normalised figures are presented on the Excel spreadsheet, lehanemacroarray.xls. The values are also presented in the order in which they appear in Tables 1-3 of the paper. Analysis of these data was then performed as described in Materials and methods in the paper.

1 2 3 4 5 6 7 8 9 10 11 12

A C1-26 1 2 3 4 5 6 7 8 9 10 C2-27 Gmm-2387* Gmm- Gmm- Gmm- Gmm-2522 Tse15b02 Gmm-1963 Gmm- Gmm- Tse29b09 Gmm-3236 Gmm- Actin 1694 2445 0596 MAP Toll Attacin 3328 0072 Scavenger Adhesion/ 2682* Iron met Chitin Toll kinase pathway Scavenger Scavenger receptor lectin GAPDH binding pathway receptor receptor B 11 12 13 14 15 16 17 18 19 20 21 22 Gmm-0048 Gmm- Tse33h03 Tse36b05 Gmm-2904 Tse37e05 Gmm-2058 Gmm- Gmm- Tse41c10 Gmm-3352 Tse44a11 Chitin 2709 Adhesion/ Opsonizati Chitin Toll Extracellular 1270 2594 JNK Serpin Toll binding Chitin lectin on binding pathway peroxidase Imd Serpin cascade pathway binding pathway C 23 24 25 26 27 28 29 30 31 32 33 34 Tse48e12 Gmm- Tse51g11 Tse59b02 Gmm-0398 Gmm-2608 Tse66g09 Tse70a12 Gmm- Gmm-3334 Tse72e04 Tse72e08 2 macro- 2945 Toll path- scavenger Chitin Scavenger JAK_STAT Binding 2766 Serpin Serpin Serpin globulin Chitin way receptor binding receptor Cascade bacteria Serpin binding D 35 36 37 38 39 40 41 42 43 44 45 46 Gmm-1329 Tse74h11 Gmm- Tse81d11 Gmm-3029 Gmm-2356 Tse84d03 Gmm- Tse91h03 Tse94c04 Gmm-0034 Gmm-2386 Chitin Chitin 2412 Binding Translation Serpin Scavenger 1598 Serine car Serpin Iron Chitin binding binding Adhesion/ bacteria regulation receptor Serine car- boxypep metabolis binding lectin boxypep m

E 47 48 49 50 51 52 53 54 55 56 57 58 Tse98g05 Gmm- Tse100h09 Tse105c10 Gmm-3262 Tse108b10 Tse112d02 Gmm- Gmm- Gmm-3348 Gmm-1799 Tse121d04 Chitin 2629 Iron metab 2 macro- Chitin Scavenger Serine endo- 2087 0033 Iron metab Oxidative Chitin binding Oxidative olism globulin binding receptor peptidase Peroxidase Serpin olism stress binding stress F neg 59 60 61 62 63 64 65 66 67 68 C3-40 Gmm- Gmm- Gmm- Gmm-3093 Tse126a11 Gmm-3156 Tse129g02 Gmm- Gmm-2843 Tse131b02 C1088* 3099 2619 0601 Chitin Extracellul Binding PPO 3307 Chitin MAP EIF Peroxidase Peroxidase Extracellul binding ar peroxid bacteria activation Iron met binding kinase ar peroxid abolism