Used of Some Parameters in Inquiry About Chronic Hepatitis C Disease

Journal of Babylon University/Pure and Applied Sciences/ No.(2)/ Vol.(21): 2013

Used Of Some Physiological Parameters In Prediction Of Chronic Hepatitis C Disease

Mohammad Hussein obaid University of Babylon- College of Science-Biology Department

Abstract An physiological study has been done for patients with hepatitis c .Levels of Ferritin,alkaline phosphate,Glutamyl oxaloacetic transminase(GOT) and Glutamyl pyruvic transminase(GPT) in sera for 30 patients and 10 healthy subjects were determined . The results showed increased in levels of Ferritin,alkaline phosphate,GPT,and GOT compared with control group.These results suggested that increases values of Ferritin and other parameters may be a maeker of chronic hepatitis c infection. Keys: Hepatitis c, Ferritin ,HC Virus . الخلصة تم عمل دراسه فسلجيه للمرضى المصابين بالتهاب الكبد الفايروسي نمط C المزمن, وذلك بتحديد مستويات Ferritin,alkaline phosphate,Glutamyl oxaloacetic transminase(GOT) and Glutamyl pyruvic transminase(GPT) في مصل ثلثين مريض مع عشرة اشخاص اصحاء .بينت الدراسة ان هنالك زيادة في مستويات كل من Ferritin,alkaline phosphate,Glutamyl oxaloacetic transminase(GOT) and Glutamyl pyruvic transminase(GPT) مقارنة مع مجموعة السيطرة . وقد اقترحت هذه النتائج ان قيم الزيادات في مستويات الفريتين والمعايير الخرى ربما تكون مؤشر للصابة بالتهاب الكبد الفايروسي نمط C المزمن . Introduction Hepatitis C is a disease affecting the liver of patients (Ryan and Ray 2004;Reddy,2010).The infection by hepatitis C virus cause chronic infection can lead to asymptomatic often which is cirrhosis and advanced scarring (fibrosis scarring of the liver) generally apparent after many years . In some cases, those with cirrhosis will go on to develop liver failure or other complications of cirrhosis(Ryan and Ray 2004). Hepatitis infection usually progresses in a predictable and slow fashion about six weeks after exposure to the virus, peoples undergo the acute phase of infection ,and this stage of illness is usually dismissed by the patients because it passes with very few, if any, signs or symptoms, therefore, the body has usually not even begun to produce antibodies specific to the virus, so a blood test may not reveal clues to infection (Iwasaki. et al.,2002) . Reduction of iron in sera has been shown to normalize liver enzyme levels, which are elevated during periods of active liver damage (Fong et al 1998). Iron depletion therapy has also been shown to improve the response to conventional medicines used to treat hepatitis. The effective way to decrease iron of sera is to have an iron loss, as occurs when donating blood. Hepatitis C patients cannot donate blood for common use, but their blood can still be removed, although it must be discarded (Fargion,et al 1997). Ferritin is a protein found inside cells that stores iron so our body can use it later. A ferritin test indirectly measures the amount of iron in blood (Hoffman .et al., 2005; McPherson and Pincus.,2007) . The aims of this study was to evaluate a possible relationship between Ferritin as well as certain parameters and infection with hepatitis C .

138 Journal of Babylon University/Pure and Applied Sciences/ No.(2)/ Vol.(21): 2013

Material and Methods 1-Blood samples: Thirty a dult patients (18 male and 12 female),their ages ranged between 19-50 years ,were prediagnostic by physician in AL-Hilla teaching hospital .Ten adult healthy subject(4 male and 6 female)served as control group.Blood samples were taken at the hospital laboratory and each sample was subjected to centrifugation , The sera were immediately stored at deep freez unit. 2-Tests: A-Ferritin test: This test was done according to Tietz (1999)by using ELISA kit(monobind company). B-GOT and GPT test: The Got and Gpt levels were measured according to Reitman and Frankel (1957) C-Phosphate alkaline test: This test was done according to Belfeild and Gold berg (1971) by using phosphate alkaline kit (Biomerieux company). 3-Statistical Analysis: The results of this work were analyzed using completely randomized design(Donald,1989).

Results All sera samples in test group showed elevated in levels of Ferritin and alkaline phosphatase compared with control group ( Table 1) .

(Table 1) Mean of Ferritin & alkaline phosphatase concentration

Parameters pa Ferritin(ng /ml) Alkaline phosphatase (U Samples Means ± S.d / l) Means ± S.d Patients (30 ) 118 ±14.90 144 ±3.87

Control (10 ) 51 ± 1 86 ± 0

Standard deveation = S.d * Table( 2 ) showed increased in levels of Got and Gpt in patients , which reached 35.48 and 43.8 IU/ ml respectively , while it was 14.7 and 23.5 IU/ ml in control group consecutively.

(Table 2 ) Mean of Liver enzymes (GOT&GPT) Concentration

Parameters GOT(iu / ml) GPT(iu / ml) Means ± S.d Means ± S.d Samples

Patients (30 ) 35.48±2.86 43.8± 0

Control (10 ) 14.7± 0 23.5± 0

139 Discussion Ferritin is a protein in the body that binds to iron; most of the iron stored in the body is bound to ferritin. Ferritin is found in the liver, spleen , skeletal muscles, and bone marrow. Only a small amount of it is found in the blood which shows how much iron is stored in ours body (Fargion. S. et al., 1997). The results (tables 1&2) showed increased levels this parameter of patients group more than the healthy group which showed normal levels and this agreed with (Di Bisceglie. et al., 1992; Haque. et al ., 1996; Fargion.et al., 1997; Ganne-Carrie. et al ., 2000; Kakizaki S.et al., 2000) who founded that Hepatitis C virus infection is significantly associated with increased serum levels of ferritin and iron and these values were directly correlated with serum concentrations of GOT, GPT, and γ-glutamyl- transpeptidase. In medicine, the presence of elevated transaminases, commonly the transaminases alanine transaminase (GPT) and aspartate transaminase (GOT), may be an indicator of liver damage (Giboney. 2005). Therefore, we measured the levels of GOT & GPT and the results revealed increases in GOT &GPT serum levels in patients group compared with control group,and this result agreed with (Halfon. et al ., 2008 ) who found that GOT and GPT increased in patients with chronic hepatitis C disease. Elevated serum (GOT), a liver enzyme that rises in response to increased oxidative stress in the liver (Iwasaki M et al 2002).

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