Channel Toxin from Parabuthus Transvaalicus
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Convergent Recruitment of Knottin and Defensin Peptide Scaffolds Into the Venom of Predatory Assassin Flies
Journal Pre-proof Weaponisation ‘on the fly’: convergent recruitment of knottin and defensin peptide scaffolds into the venom of predatory assassin flies Jiayi Jin, Akello J. Agwa, Tibor G. Szanto, Agota Csóti, Gyorgy Panyi, Christina I. Schroeder, Andrew A. Walker, Glenn F. King PII: S0965-1748(19)30425-4 DOI: https://doi.org/10.1016/j.ibmb.2019.103310 Reference: IB 103310 To appear in: Insect Biochemistry and Molecular Biology Received Date: 8 October 2019 Revised Date: 12 December 2019 Accepted Date: 16 December 2019 Please cite this article as: Jin, J., Agwa, A.J., Szanto, T.G., Csóti, A., Panyi, G., Schroeder, C.I, Walker, A.A., King, G.F., Weaponisation ‘on the fly’: convergent recruitment of knottin and defensin peptide scaffolds into the venom of predatory assassin flies Insect Biochemistry and Molecular Biology, https:// doi.org/10.1016/j.ibmb.2019.103310. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2019 Published by Elsevier Ltd. Intended for submission to Insect Biochemistry and Molecular Biology Special Issue on Active Peptides in Insects Weaponisation ‘on the fly’: convergent recruitment of knottin and defensin peptide scaffolds into the venom of predatory assassin flies Jiayi Jin 1, Akello J. -
Production of Scorpion Antivenom From
Received: March 6, 2007 J. Venom. Anim. Toxins incl. Trop. Dis. Accepted: May 9, 2007 V.13, n.4, p.844-856, 2007. Abstract published online: May 9, 2007 Original paper. Full paper published online: November 30, 2007 ISSN 1678-9199. COMPARISON OF PROTEINS, LETHALITY AND IMMUNOGENIC COMPOUNDS OF Androctonus crassicauda (OLIVIER, 1807) (SCORPIONES: BUTHIDAE) VENOM OBTAINED BY DIFFERENT METHODS OZKAN O. (1, 2), KAR S. (2), GÜVEN E. (2) ERGUN G. (3) (1) Refik Saydam Hygiene Center, Ankara, Turkey; (2) Department of Entomology, Faculty of Veterinary Medicine, Ankara, Turkey; (3) Department of Statistics, Faculty of Sciences, Hacettepe University, Ankara, Turkey. ABSTRACT: Scorpions are venomous arthropods of the class Arachnida and are considered relatives of spiders, ticks and mites. There are approximately 1,500 species of scorpions worldwide, which are characterized by an elongated body and a segmented tail that ends in a venomous stinger. No specific treatment is available for scorpion envenomation, except for the use of antivenom. The current study aimed at comparing protein content and lethality of Androctonus crassicauda venom extracted by two different methods (electric stimulation and maceration of telsons). The LD50 calculated by probit analysis was 1.1mg/kg for venom obtained by electric stimulation and 39.19mg/kg for venom obtained by maceration of telsons. In the electrophoretic analysis, protein bands of the venom sample obtained by electric stimulation were between 12 and 53kDa (total: five bands), and those of venom extracted by maceration appeared as multiple protein bands, relative to the other venom sample. Low-molecular-weight proteins, revealed by western blotting, played an important immunogenic role in the production of antivenom. -
Scorpion Toxins Targeted Against the Sarcoplasmic Reticulum Ca2+-Release Channel of Skeletal and Cardiac Muscle
Proc. Nati. Acad. Sci. USA Vol. 89, pp. 12185-12189, December 1992 Physiology Scorpion toxins targeted against the sarcoplasmic reticulum Ca2+-release channel of skeletal and cardiac muscle (ryanodine receptors/Pandinus imperator venom/planar bilayer/ventricular myocytes/Ca2+ indicator) HECTOR H. VALDIVIA*t, MARK S. KIRBYf, W. JONATHAN LEDERER*, AND ROBERTO CORONADO*t *Department of Physiology, University of Wisconsin School of Medicine, Madison, WI 53706; and tDepartment of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201 Communicated by Michael V. L. Bennett, September 21, 1992 ABSTRACT We report the purification of two peptides, peratoxin inhibitor (IpTxi)] or activated [imperatoxin activa- called "imperatoxin inhibitor" and "imperatoxin activator," tor (IpTxa)] ryanodine receptors of skeletal and cardiac from the venom of the scorpion Pandinus imperator targeted muscle. Part of these results have been communicated in an against ryanodine receptor Ca2+-release channels. Impera- abstract form (8). toxin inhibitor has a Mr of 10,500, inhibits [3Hjryanodine binding to skeletal and cardiac sarcoplasmic reticulum with an EDso of 10 nM, and blocks openings of skeletal and cardiac EXPERIMENTAL PROCEDURES Ca2+-release channels incorporated into planar bilayers. In Purification of Scorpion Toxins. Lyophilized P. imperator whole-cell recordings of cardiac myocytes, imperatoxin inhib- venom was obtained from Latoxan (Rosans, France). Venom itor decreased twitch amplitude and intracellular Ca2+ tran- (50 mg per batch) was extracted in 2-3 ml of deionized water sients, suggesting a selective blockade of Ca2+ release from the and chromatographed on a column (1. 5 x 125 cm) of Sepha- sarcoplasmic reticulum. Imperatoxin activator has a Mr of dex G-50 fine. -
Multiple Actions of Φ-LITX-Lw1a on Ryanodine Receptors Reveal a Functional Link Between Scorpion DDH and ICK Toxins
Multiple actions of φ-LITX-Lw1a on ryanodine receptors reveal a functional link between scorpion DDH and ICK toxins Jennifer J. Smitha, Irina Vettera, Richard J. Lewisa, Steve Peigneurb, Jan Tytgatb, Alexander Lamc, Esther M. Gallantc, Nicole A. Beardd, Paul F. Alewooda,1,2, and Angela F. Dulhuntyc,1,2 aChemical and Structural Biology, Institute for Molecular Biosciences, University of Queensland, St. Lucia, QLD 4072, Australia; bLaboratory of Toxicology, University of Leuven, 3000 Leuven, Belgium; cDepartment of Molecular Bioscience, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia; and dDiscipline of Biomedical Sciences, Faculty of Education, Science, Technology and Mathematics, University of Canberra, Canberra, ACT 2601, Australia Edited by Clara Franzini-Armstrong, University of Pennsylvania Medical Center, Philadelphia, PA, and approved April 23, 2013 (received for review August 26, 2012) We recently reported the isolation of a scorpion toxin named U1- mutations, atypical posttranslational modifications of RyRs, liotoxin-Lw1a (U1-LITX-Lw1a) that adopts an unusual 3D fold termed including hyperphosphorylation and S-nitrosylation, have been the disulfide-directed hairpin (DDH) motif, which is the proposed implicated in heart failure and pathologic muscle fatigue (10– evolutionary structural precursor of the three-disulfide-containing 12). Because of their wide distribution, there is mounting evidence inhibitor cystine knot (ICK) motif found widely in animals and plants. to suggest that dysregulation of RyRs also plays a role in a plethora Here we reveal that U1-LITX-Lw1a targets and activates the mam- of other disease states, including chronic pain, neurodegenerative malian ryanodine receptor intracellular calcium release channel (RyR) disorders such as Alzheimer’s disease, and intellectual deficit (13– with high (fM) potency and provides a functional link between DDH 15). -
Arachnologische Mitteilungen
ZOBODAT - www.zobodat.at Zoologisch-Botanische Datenbank/Zoological-Botanical Database Digitale Literatur/Digital Literature Zeitschrift/Journal: Arachnologische Mitteilungen Jahr/Year: 2012 Band/Volume: 43 Autor(en)/Author(s): Chiarle Alberto, Kovarik Frantisek, Levi Lisa, Gavetti Elena Artikel/Article: The scorpion collections (Arachnida, Scorpiones) held in the Museo Regionale di Scienze Naturali of Turin (Italy) 17-23 2 © Biodiversity Heritage Library, http://www.biodiversitylibrary.org/; Arachnologische Mitteilungen 43: 17-23 Nuremberg, July 2012 The scorpion collections (Arachnida, Scorpiones) held in the Museo Regionale di Scienze Naturali of Turin (Italy) Alberto Chiarle, Frantisek Kovarik, Lisa Levi & Elena Gavetti doi;10.5431/aramit4307 Abstract: Data and considerations about the history and contents of the scorpion collection housed in the Museo Regionale di Scienze Naturali of Turin (MRSN) are reported. Information on type material and important historical specimens are provided, as well as biographical notes about the major zoologists of the museum. Key words: Borelli, MZUT, MRSN, scorpiology,Turin collection history In 1978 the public administration of the Regione the museum collections were neglected. Specimens in Piemonte founded the ‘Museo Regionale di Scienze ethanol suffered and exchanges, loans and systematic Naturali’ (MRSN) in Turin. Since 1980, the zoologi- studies on specimens were interrupted. In 1968, under cal treasures kept by the Zoological Museum of the Professor Umberto Parenti’s guidance and thanks to Turin University (MZUT) have been entrusted to the the curators, the MZUT was reorganized. MRSN. The MZUT is one of the oldest and most Since 1980 a large number of transfers have taken important Italian university museums. It was founded place involving all the collections. -
Bioinformatic Characterizations and Prediction of K+ and Na+ Ion Channels Effector Toxins
Bioinformatic characterizations and prediction of K+ and Na+ ion channels effector toxins. Rima Soli, Belhassen Kaabi, Mourad Barhoumi, Mohamed El-Ayeb, Najet Srairi-Abid To cite this version: Rima Soli, Belhassen Kaabi, Mourad Barhoumi, Mohamed El-Ayeb, Najet Srairi-Abid. Bioinformatic characterizations and prediction of K+ and Na+ ion channels effector toxins.. BMC Pharmacology, BioMed Central, 2009, 9, pp.4. 10.1186/1471-2210-9-4. pasteur-00612552 HAL Id: pasteur-00612552 https://hal-riip.archives-ouvertes.fr/pasteur-00612552 Submitted on 2 Aug 2011 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. BMC Pharmacology BioMed Central Research article Open Access Bioinformatic characterizations and prediction of K+ and Na+ ion channels effector toxins Rima Soli†1, Belhassen Kaabi*†1,3, Mourad Barhoumi1, Mohamed El-Ayeb2 and Najet Srairi-Abid2 Address: 1Laboratory of Epidemiology and Ecology of Parasites, Institut Pasteur de Tunis, Tunis, Tunisia, 2Laboratory of Venom and Toxins, Institut Pasteur de Tunis, Tunis, Tunisia and 3Research and Teaching Building, -
Maurocalcine-Derivatives As Biotechnological Tools for The
Maurocalcine-derivatives as biotechnological tools for the penetration of cell-impermeable compounds Narendra Ram, Emilie Jaumain, Michel Ronjat, Fabienne Pirollet, Michel de Waard To cite this version: Narendra Ram, Emilie Jaumain, Michel Ronjat, Fabienne Pirollet, Michel de Waard. Maurocalcine- derivatives as biotechnological tools for the penetration of cell-impermeable compounds: Technological value of a scorpion toxin. de Lima, M.E.; de Castro Pimenta, A.M.; Martin-Eauclaire, M.F.; Bendeta Zengali, R.; Rochat, H. E. Animal Toxins: state of the art: Perspectives in Health and Biotechnology., Editora UFMG Belo Horizonte, Brazil, pp.715-732, 2009. inserm-00515224 HAL Id: inserm-00515224 https://www.hal.inserm.fr/inserm-00515224 Submitted on 5 Sep 2013 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Maurocalcine-derivatives as biotechnological tools for the penetration of cell-impermeable compounds by Narendra Ram1,2, Emilie Jaumain1,2, Michel Ronjat1,2, Fabienne Pirollet1,2 & Michel De Waard1,2* 1 INSERM, U836, Team 3: Calcium channels, functions and pathologies, BP 170, Grenoble Cedex 9, F-38042, France 2 Université Joseph Fourier, Institut des Neurosciences, BP 170, Grenoble Cedex 9, F-38042, France Running title: Technological value of a scorpion toxin *To whom correspondence should be sent: Dr. -
Venom Week 2012 4Th International Scientific Symposium on All Things Venomous
17th World Congress of the International Society on Toxinology Animal, Plant and Microbial Toxins & Venom Week 2012 4th International Scientific Symposium on All Things Venomous Honolulu, Hawaii, USA, July 8 – 13, 2012 1 Table of Contents Section Page Introduction 01 Scientific Organizing Committee 02 Local Organizing Committee / Sponsors / Co-Chairs 02 Welcome Messages 04 Governor’s Proclamation 08 Meeting Program 10 Sunday 13 Monday 15 Tuesday 20 Wednesday 26 Thursday 30 Friday 36 Poster Session I 41 Poster Session II 47 Supplemental program material 54 Additional Abstracts (#298 – #344) 61 International Society on Thrombosis & Haemostasis 99 2 Introduction Welcome to the 17th World Congress of the International Society on Toxinology (IST), held jointly with Venom Week 2012, 4th International Scientific Symposium on All Things Venomous, in Honolulu, Hawaii, USA, July 8 – 13, 2012. This is a supplement to the special issue of Toxicon. It contains the abstracts that were submitted too late for inclusion there, as well as a complete program agenda of the meeting, as well as other materials. At the time of this printing, we had 344 scientific abstracts scheduled for presentation and over 300 attendees from all over the planet. The World Congress of IST is held every three years, most recently in Recife, Brazil in March 2009. The IST World Congress is the primary international meeting bringing together scientists and physicians from around the world to discuss the most recent advances in the structure and function of natural toxins occurring in venomous animals, plants, or microorganisms, in medical, public health, and policy approaches to prevent or treat envenomations, and in the development of new toxin-derived drugs. -
A Global Accounting of Medically Significant Scorpions
Toxicon 151 (2018) 137–155 Contents lists available at ScienceDirect Toxicon journal homepage: www.elsevier.com/locate/toxicon A global accounting of medically significant scorpions: Epidemiology, major toxins, and comparative resources in harmless counterparts T ∗ Micaiah J. Ward , Schyler A. Ellsworth1, Gunnar S. Nystrom1 Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA ARTICLE INFO ABSTRACT Keywords: Scorpions are an ancient and diverse venomous lineage, with over 2200 currently recognized species. Only a Scorpion small fraction of scorpion species are considered harmful to humans, but the often life-threatening symptoms Venom caused by a single sting are significant enough to recognize scorpionism as a global health problem. The con- Scorpionism tinued discovery and classification of new species has led to a steady increase in the number of both harmful and Scorpion envenomation harmless scorpion species. The purpose of this review is to update the global record of medically significant Scorpion distribution scorpion species, assigning each to a recognized sting class based on reported symptoms, and provide the major toxin classes identified in their venoms. We also aim to shed light on the harmless species that, although not a threat to human health, should still be considered medically relevant for their potential in therapeutic devel- opment. Included in our review is discussion of the many contributing factors that may cause error in epide- miological estimations and in the determination of medically significant scorpion species, and we provide suggestions for future scorpion research that will aid in overcoming these errors. 1. Introduction toxins (Possani et al., 1999; de la Vega and Possani, 2004; de la Vega et al., 2010; Quintero-Hernández et al., 2013). -