OIE Reference Laboratory Reports s9

OIE Reference Laboratory Reports Activities in 2011

Name of disease (or topic) for Dourine which you are a designated OIE Reference Laboratory:

Address of laboratory: 109428 Moscow, Ryazanskiy prosp. 24 – 1, VIEV RUSSIA

Tel.: +7 (495) 785 – 84 - 27

Fax: +7 (495) 995 – 88 - 63

e-mail address: [email protected]

website: www.viev.ru

Name (including Title and Prof. Vyacheslav T. Zablotsky Position) of Head of Laboratory (Responsible Official):

Name(including Title and Prof. Vyacheslav T. Zablotsky Position) of OIE Reference Expert:

Name (including Title and Position) of writer of this report (if different from above):

Annual reports of OIE Reference Centres, 2011 1 Dourine

Part I: Summary of general activities related to the disease

1. Test(s) in use/or available for the specified disease/topic at your laboratory

Test For Specificity Total

Serological assay/ELISA Antibody Group 950

Serological assay/CFT Antibody Group 950

Parasitological assay/microscopy Trypanosomes - 100

Parasitological assay/bioprobe in mice Trypanosomes - 100

2. Production and distribution of diagnostic reagents

Amount supplied nationally Amount supplied to other Type of reagent (including for own use) countries

Control positive serum 500 ml 200 ampoules of 1ml

Antigens for ELISA test 500 ampoules of 1ml 200 ampoules of 1ml

Antigens for CFT test 500 ampoules of 1ml 200 ampoules of 1ml

Diagnostic reagents and controls for fulfilment Dourine serological assays (ELISA, CFT) were produced and tested in-house at a quantity sufficient to test all samples in the reporting period.

Our laboratory has supplied reagents for ELISA and CFT to veterinary diagnostic laboratories within Russia and to other OIE Member countries.

Part II: Activities specifically related to the mandate of OIE Reference Laboratories

3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines

a) Establishment and maintenance of a network with other OIE Reference Laboratories designated for the same pathogen or disease and organisation of regular inter-laboratory proficiency testing to ensure comparability of results

b) Organisation of inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results

Comparative trials on diagnostic value of commercial antigens produced in different countries for the diagnosis of dourine.

4. Preparation and supply of international reference standards for diagnostic tests or vaccines

The following strains are maintained at the cryobank of the Laboratory: five strains of T. equiperdum: “Bordeaux”, “Antwerp”, “Alfort”, “Hamburg”, “SVP”; three strains of T. evansi; and also one strain of Babesia rodhaini.

5. Research and development of new procedures for diagnosis and control

Research on PCR development for differential diagnosis of T. equiperdum (dourine) and T. evansi (surra) were continued.

6. Collection, analysis and dissemination of epizootiological data relevant to international disease control

Dourine epizootology situation monitoring for Russia is carried out at the Laboratory.

7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned

8. Provision of consultant expertise to OIE or to OIE Member Countries

Five samples of equine sera sent from Germany have been tested: five samples are negative.

9. Provision of scientific and technical training to personnel from other OIE Member Countries

10. Provision of diagnostic testing facilities to other OIE Member Countries

During 2011, 154 samples of imported horses were submitted for primary diagnosis on Dourine from 6 countries . Details are as follows:

Summary of samples collected and received in 2011

Country No. of samples CFT test

Positive Negative

Estonia 15 - 15 Latvia 10 - 10 Moldova 4 - 4 Poland 80 - 80 Belorussia 15 - 15 Ukraine 30 - 30

Total 154 0 154

11. Organisation of international scientific meetings on behalf of OIE or other international bodies

12. Participation in international scientific collaborative studies

13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)

 Presentations at international conferences and meetings

YADAV S. C., KUMAR R. , INOUE N. , YUROV K. , ZABLOTSKY V. , DESQUESNES M., LUN Z. R. , TOURATIER L. // “Detection and containment of Trypanozoon infections in equines throughout Asia in present times”// Proc. of. 12th International Congress of WEVA, 2 – 5th November, Hyderabad, India, 2011, pp. in press.

 Scientific publications in peer-reviewed journals

Gulyukin M. I., Yurov K. P., Zablotsky V. T. // “Scientific and Practical Activity of OIE Reference Laboratories”. //J. “Veterinary” (in Russian), vol. 7, pp. 18 - 20, 2011.

 Other communications

Zablotsky V. T. // Article in Book : «Parasitic diseases of animals/ Chapter 2 - Current methods of control of infectious and invasious diseases of animals ». // Moscow Press, pp. 154 – 200, 2011.

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Annual reports of OIE Reference Centres, 2011 4