<p> OIE Reference Laboratory Reports Activities in 2011</p><p>Name of disease (or topic) for Dourine which you are a designated OIE Reference Laboratory:</p><p>Address of laboratory: 109428 Moscow, Ryazanskiy prosp. 24 – 1, VIEV RUSSIA</p><p>Tel.: +7 (495) 785 – 84 - 27</p><p>Fax: +7 (495) 995 – 88 - 63</p><p> e-mail address: [email protected]</p><p> website: www.viev.ru</p><p>Name (including Title and Prof. Vyacheslav T. Zablotsky Position) of Head of Laboratory (Responsible Official):</p><p>Name(including Title and Prof. Vyacheslav T. Zablotsky Position) of OIE Reference Expert:</p><p>Name (including Title and Position) of writer of this report (if different from above):</p><p>Annual reports of OIE Reference Centres, 2011 1 Dourine</p><p>Part I: Summary of general activities related to the disease</p><p>1. Test(s) in use/or available for the specified disease/topic at your laboratory</p><p>Test For Specificity Total</p><p>Serological assay/ELISA Antibody Group 950</p><p>Serological assay/CFT Antibody Group 950</p><p>Parasitological assay/microscopy Trypanosomes - 100</p><p>Parasitological assay/bioprobe in mice Trypanosomes - 100</p><p>2. Production and distribution of diagnostic reagents </p><p>Amount supplied nationally Amount supplied to other Type of reagent (including for own use) countries</p><p>Control positive serum 500 ml 200 ampoules of 1ml</p><p>Antigens for ELISA test 500 ampoules of 1ml 200 ampoules of 1ml </p><p>Antigens for CFT test 500 ampoules of 1ml 200 ampoules of 1ml</p><p>Diagnostic reagents and controls for fulfilment Dourine serological assays (ELISA, CFT) were produced and tested in-house at a quantity sufficient to test all samples in the reporting period.</p><p>Our laboratory has supplied reagents for ELISA and CFT to veterinary diagnostic laboratories within Russia and to other OIE Member countries.</p><p>Part II: Activities specifically related to the mandate of OIE Reference Laboratories</p><p>3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines</p><p> a) Establishment and maintenance of a network with other OIE Reference Laboratories designated for the same pathogen or disease and organisation of regular inter-laboratory proficiency testing to ensure comparability of results</p><p>None.</p><p> b) Organisation of inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results</p><p>Comparative trials on diagnostic value of commercial antigens produced in different countries for the diagnosis of dourine.</p><p>Annual reports of OIE Reference Centres, 2011 2 Dourine</p><p>4. Preparation and supply of international reference standards for diagnostic tests or vaccines</p><p>The following strains are maintained at the cryobank of the Laboratory: five strains of T. equiperdum: “Bordeaux”, “Antwerp”, “Alfort”, “Hamburg”, “SVP”; three strains of T. evansi; and also one strain of Babesia rodhaini.</p><p>5. Research and development of new procedures for diagnosis and control </p><p>Research on PCR development for differential diagnosis of T. equiperdum (dourine) and T. evansi (surra) were continued.</p><p>6. Collection, analysis and dissemination of epizootiological data relevant to international disease control</p><p>Dourine epizootology situation monitoring for Russia is carried out at the Laboratory.</p><p>7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned</p><p>None.</p><p>8. Provision of consultant expertise to OIE or to OIE Member Countries</p><p>Five samples of equine sera sent from Germany have been tested: five samples are negative.</p><p>9. Provision of scientific and technical training to personnel from other OIE Member Countries</p><p>None.</p><p>10. Provision of diagnostic testing facilities to other OIE Member Countries</p><p>During 2011, 154 samples of imported horses were submitted for primary diagnosis on Dourine from 6 countries . Details are as follows:</p><p>Summary of samples collected and received in 2011</p><p>Country No. of samples CFT test</p><p>Positive Negative</p><p>Estonia 15 - 15 Latvia 10 - 10 Moldova 4 - 4 Poland 80 - 80 Belorussia 15 - 15 Ukraine 30 - 30</p><p>Total 154 0 154</p><p>Annual reports of OIE Reference Centres, 2011 3 Dourine</p><p>11. Organisation of international scientific meetings on behalf of OIE or other international bodies</p><p>None.</p><p>12. Participation in international scientific collaborative studies</p><p>None.</p><p>13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences) </p><p> Presentations at international conferences and meetings</p><p>YADAV S. C., KUMAR R. , INOUE N. , YUROV K. , ZABLOTSKY V. , DESQUESNES M., LUN Z. R. , TOURATIER L. // “Detection and containment of Trypanozoon infections in equines throughout Asia in present times”// Proc. of. 12th International Congress of WEVA, 2 – 5th November, Hyderabad, India, 2011, pp. in press.</p><p> Scientific publications in peer-reviewed journals</p><p>Gulyukin M. I., Yurov K. P., Zablotsky V. T. // “Scientific and Practical Activity of OIE Reference Laboratories”. //J. “Veterinary” (in Russian), vol. 7, pp. 18 - 20, 2011.</p><p> Other communications</p><p>Zablotsky V. T. // Article in Book : «Parasitic diseases of animals/ Chapter 2 - Current methods of control of infectious and invasious diseases of animals ». // Moscow Press, pp. 154 – 200, 2011. </p><p>______</p><p>Annual reports of OIE Reference Centres, 2011 4</p>