Standard Operating Procedure s22

Standard Operating Procedure

Acrolein

Print a copy and insert into your Laboratory Safety Manual and Chemical Hygiene Plan. Refer to instructions for assistance.

Department: Chemistry

Date SOP was written: 11/29/2012

Date SOP was approved by PI/lab supervisor: 11/29/2012

Principal Investigator: Richmond Sarpong

Internal Lab Safety Coordinator/Lab Manager: Rebecca Murphy

Lab Phone: 510-643-2485

Office Phone: 510-643-6312

Emergency Contact: Richmond Sarpong (626)-644-2407 (Name and Phone Number)

Location(s) covered by this SOP: Latimer 834,836,837,838,839,842,844,847,907 (Building/Room Number)

Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class

Purpose

Acrolein (also known as 2-propenal) is a hazardous liquid that is very toxic and corrosive. It is a highly flammable liquid or vapor. It may be fatal if ingested, inhaled, or absorbed through the skin. It may cause respiratory tract, skin, and eye irritation. It can cause severe burns to skin and eyes with permanent damage. Acrolein is mainly used as an herbicide and a biocide in the oil and gas industry. It is also used as a fixative in electron microscopy imaging of biological specimens.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 107-02-8

Class: Highly flammable, very toxic, corrosive, carcinogen

Molecular Formula: C3H4O

Form (physical state): Liquid

Acrolein 1/08/2013 Color: Colorless, yellow

Boiling Point: 51-53 °C

Flash Point: -29 °C -closed cup

Lower Explosion Limit 2.8%(V)

Upper Explosion Limit 31%(V)

Potential Hazards/Toxicity

Acrolein is a highly flammable liquid or vapor. Carbon oxides may form under fire conditions. May be fatal if inhaled, ingested, or absorbed through the skin. May cause respiratory tract, skin, and eye irritation. Causes burns to skin and eyes with severe or permanent eye damage. May cause allergic skin reaction. It is extremely destructive to the tissue of the mucous membranes and upper respiratory tract. May cause coughing, shortness of breath, pulmonary edema, chemical pneumonitis, nausea, and headache. Ingestion may cause perforation to the esophagus and stomach. May cause cancer. Acrolein has a Cal-OSHA Permissible Exposure Limit (PEL) of 0.25 mg/m3.

Engineering Controls

Acrolein should be used in a glove box or in a closed system in a certified chemical fume hood.

Personal Protective Equipment (PPE)

NOTE: Lab-specific information on PPE selection may be included in Lab Specific Instructions.

Respiratory protection

NOTE: Lab personnel intending to use/wear a respirator mask must be trained and fit-tested by EH&S. This is a regulatory requirement.

Refer to 8 CCR 5144 for selection of respirators. A respiratory protection program that meets 8 CCR 5144 must be followed whenever workplace conditions warrant use of a respirator.

Respirators should be used only under any of the following circumstances:

 As a last line of defense (i.e., after engineering and administrative controls have been exhausted).  When Permissible Exposure Limit (PEL) has exceeded or when there is a possibility that PEL will be exceeded.  Regulations require the use of a respirator.  An employer requires the use of a respirator.  There is potential for harmful exposure due to an atmospheric contaminant (in the absence of PEL)  As PPE in the event of a chemical spill clean-up process

Hand Protection

Acrolein 1/08/2013 Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands.

For glove selection, go to: http://ehs.berkeley.edu/hs/63-laboratory-safety/94-glove-selection- and-usage.html

Eye Protection

Safety glasses with side shields or tightly fitting safety goggles. Use face shield (8-inch minimum) when appropriate. Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU) or ANSI Z87.1.

Skin and Body Protection

Long pants, closed-toed and closed-heeled shoes, cotton-based clothing/attire, and flame resistant (where appropriate) lab coat must be worn for protecting against chemical hazards.

Additional PPE may be required if procedures or processes present additional risk. It is the responsibility of the PI to ensure that any additional PPE requirements are identified and communicated to research staff.

Storage

Use unbreakable secondary containment for the storage of acutely toxic chemicals. If the materials are volatile (or could react with moisture or air to form volatile toxic compounds), containers should be in a ventilated storage area. All containers of acutely toxic chemicals should be clearly labeled with chemical composition, known hazards, and warnings for handling.

Chemicals that can combine to make toxic materials (e.g., acids and inorganic cyanides, which can generate hydrogen cyanide) should not be stored in the same secondary containment. Chemicals that have a limited shelf life need to be tracked and monitored. Chemicals that require refrigeration should be stored appropriately.

Spill Response and Exposure Response

Before beginning work with chemicals, review the relevant SOPs, Safety Data Sheets, and other chemical safety resources. Develop specific procedures for emergency response and chemical exposure or injury to staff, including any special first aid measures required for the relevant chemicals.

Spill and Accident Procedure

Note: These responses may not apply to all acutely toxic chemicals. Please review specific chemical SOPs and SDSs for specific response to spills and accidents. Always dial 911 and 510-642-9090 for assistance.

Chemical Spill Dial 911

Acrolein 1/08/2013 Spill – Assess the extent of danger. Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.). Dial 911 and 510-642-9090 for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S immediately. Follow up with a call to 510-642-9090 to report the incident.

Chemical Splash Into Eyes – Immediately rinse eyeballs and inner surfaces of eyelids with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S immediately. Follow up with a call to 510- 642-9090 to report the incident.

Medical Emergency Dial 911

Life Threatening Emergency, After Hours, Weekends and Holidays – Dial 911 or go to the nearest emergency room. Note: All serious injuries must be reported to EH&S within 8 hours. Follow up with a call to 510-642-9090 to report the incident.

Non-Life Threatening Emergency – Go to the Occupational Health Facility (Tang Health Center). After hours go to the nearest emergency room. Note: All serious injuries must be reported to EH&S within 8 hours. Follow up with a call to 510-642-9090 to report the incident.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Go to the Occupational Health Facility (Tang Health Center). After hours go to the nearest emergency room. Note: All needle stick/puncture exposures must be reported to EH&S within 8 hours. Follow up with a call to 510-642-9090 to report the incident.

Hazardous Waste Disposal

General hazardous waste disposal guidelines:

Label Waste

 Label all containers with the label provided at http://ehs.berkeley.edu/hm/279-new- hazardous-waste-program-hwp.html. See the EH&S Fact Sheet, “ H azardous W a s te Management ” for general instructions on procedures for disposing of hazardous waste.

Dispose of Waste

 Dispose of regularly generated chemical waste within six (6) months.  Call EH&S for questions

Safety Data Sheet (SDS) Location

Acrolein 1/08/2013 SDS can be accessed online at http://ucmsds.com

Acrolein 1/08/2013 Protocol/Procedure CAS#: 107-02-8

Chemical Storage If not in use Acrolein containers have to be safely stored in the ventilated flammable storage cabinets.

Store and transport Acrolein containers in secondary containment (for example polyethylene bottle carrier).

Keep storage container closed (air tight) when not in use.

Maintain the smallest amount necessary for ongoing work. Use in the smallest practical quantities for the experiment being performed.

An opened container can be stored for a maximum of 6 months. Dispose of unused amounts after that period of time has passed (or if peroxides are found to be present by testing).

Acrolein is a highly flammable liquid and vapor that is used as a reagent in organic reactions Reactions may be very rapid and violent. Any resulting waste from use of acrolein should be collected and disposed of as hazardous waste.

Preparation Acrolein should only be handled in a fully functioning fume hood. Care should be taken to keep acrolein vapors inside the fume hood to prevent the buildup of highly flammable vapors outside the hood

This compound is an irritant and extremely toxic. The vapor can cause flash fires and peroxides can form upon standing so this reagent should be kept away from heat, flame, ignition sources, and sunlight. It should be kept away from acids, metal salts, oxidants, amines, or weak acid conditions such as nitrogen or carbon oxide gases.

Know the location of the nearest fire extinguisher, eyewash, and safety shower before beginning work.

Never work alone. Make sure there is another worker present who is also trained in the Acrolein SOP.

Acrolein 1/08/2013 Procedure/Use Scale Engineering Controls/Equipment PPE (eye, Procedure Steps and Precautions face, gloves, clothing) Acrolein is used in This All work using Acrolein must be performed Eye Acrolein is flammable. Acrolein is the lab as a compound in a ventilated fume hood. protection: a known carcinogen, toxic by reagent in can be Wear tight- inhalation and very hazardous by chemical handled in Eliminate ignition sources such as open fitting safety skin contact. reactions. quantities flames, hot surfaces, steam baths, static goggles or from <1mL to 500mL. electricity, and operation of mechanical and safety Use in a fume hood and cap electrical equipment that is not intrinsically glasses with container when not in use. safe. side shields. Draw out necessary amount with as Ensure proper grounding and avoid Face little exposure to ambient moisture creating static electricity. Be sure to ground protection: as possible. metal containers when transferring Wear a face flammable liquids. shield when Dispose of Acrolein-contaminated handling gloves and glassware in sealed containers containers. of Acrolein that are not behind a lab hood sash or blast shield.

Gloves: Use 0.3 mm Butyl Rubber, Latex or Nitrile Gloves.For extended or heavy use double glove

Acrolein 1/08/2013 using same.

Clothing: Wear fire/flame resistant lab coat (100% cotton based); cotton based clothing/attir e; full length pants or equivalent; and close- toed, close- heeled shoes. Notes Any deviation from this SOP requires approval from PI.

Acrolein 1/08/2013 Documentation of Training (signature and initials of all users is required)

 Prior to conducting any work with Acutely Toxic Chemicals, designated personnel must provide training to his/her laboratory personnel specific to the hazards involved in working with the specific chemical(s) used, work area decontamination, and emergency procedures.

 The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer.

I have read and understand the content of this SOP:

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Acrolein 9 Date: 9/13/2012

UCLA- EH&S Written By CC/Reviewed By: date.

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Acrolein 10 Date: 9/13/2012

UCLA- EH&S Written By CC/Reviewed By: Acrolein 11 Date: 9/13/2012

UCLA- EH&S Written By CC/Reviewed By: