S E C T I F1. Type of Antibody Production: O N ☐ Monoclonal (ascites) F A Have you attempted to use in vitro methods? N T ☐ No I If no, this must be justified and approved by the IACUC: B O Click here to enter text. D Y
/ ☐ Yes Was this successful: A Click here to enter text. S C I Justify if you have tried the in vitro method but are not using it: T Click here to enter text. E S
P ☐ Polyclonal R O F2. Immunization Method(s) D U Adjuvant Volume Total NumberC Antigens Initial Booster Route/Site of Per Total T Immunization Immunization Site ImmunizationsI Click here to enter Click here to Click here to Click here to Click here Click here to Click here toO text. enter text. enter text. enter text. to enter enter text. enter text. N Click here to enter Click here to Click here to Click here to Click here Click here to Click here to text. enter text. enter text. enter text. to enter enter text. enter text. Click here to enter Click here to Click here to Click here to Click here Click here to Click here to text. enter text. enter text. enter text. to enter enter text. enter text. Click here to enter Click here to Click here to Click here to Click here Click here to Click here to text. enter text. enter text. enter text. to enter enter text. enter text. Click here to enter Click here to Click here to Click here to Click here Click here to Click here to text. enter text. enter text. enter text. to enter enter text. enter text. Click here to enter Click here to Click here to Click here to Click here Click here to Click here to text. enter text. enter text. enter text. to enter enter text. enter text.
1 Syracuse University November 2016 F3. Description of Immunization Method(s):
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F3a. How will antigen/adjuvant mixtures be prepared:
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F3b. How animals will be prepped for immunization:
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F3c. How animals will be restrained:
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F3d. Immunization technique/method
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F3e. Time interval between initial and booster immunizations:
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F4. Methods for harvesting antibodies:
Tissue / Fluid Harvested Method Amount / Volume Frequency
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2 Syracuse University November 2016 F5. Description of Antibody Harvesting Methods(s):
F5a. How will animals be prepped for harvesting antibodies?
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F5b. How will animals be restrained?
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F5c. Time interval between last immunization and the first collection:
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F5d. Number of times tissue/fluid will be collected (consult attending veterinarian for appropriate number):
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F5e. Time interval between collection.
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3 Syracuse University November 2016