Supplemental Figure

Pyramiding of AtHDG11 and Cry2Aa2 into pepper (Capsicum annuum L.) enhances drought tolerance and insect resistance without yield decrease

Supplemental Figure

Supplemental Fig. 1 a PCR analysis of T1 putative transgenic pepper plants: Lane 1, 2 Kb marker; lane 2, pCAMBIA1301-AtEDT1/HDG11-Cry2Aa2-PIM plasmid; lane 3, non-transgenic plant (WT); lanes 4–

15, randomly choosed 12 plants of T1 generation putative transformants used for result presentation, two bands of approximately 2100 bp and 1860 bp were obtained in the 10 progenies, while other 2 progenies without, indicate transgene had a co-segregation in T1 generation pepper plants. b RT-PCR analysis of

AtEDT1/HDG11 and Cry2Aa2 gene expression pattern in 4-week-old T1 transgenic lines, Actin serve as an internal standard. Lane 1, no cDNA template; lane 2, non-transgenic (WT) plant; line 3 T1 generation null transgene plant; lanes 4-6, T1 transgenic plants (T). Result indicated AtEDT1/HDG11 and Cry2Aa2 expressed in T1 transgenic lines, but not the in non-transgenic (WT) plant and T1 generation null transgene plant.

Supplemental Fig. 2 The fruit weight and components of transgenic (T) and non-transgenic (WT) pepper fruit under drought stress conditions. a Cross section of transgenic (T) and non-transgenic (WT) pepper fruit. Pepper fruit were picked from same relative position. Bar = 2 cm. b to d. Fruit length (b), fruit diameter (c), and fruit pericarp thickness (d) of transgenic (T) and non-transgenic (WT) pepper fruit.

1 Values are mean ± SD (n= 15, *p < 0.05, ** p < 0.01)

Supplemental Fig. 3 The cross section of transgenic (T) and non-transgenic (WT) pepper mature fruit. The cells in transgenic line (T) is larger than that of the non-transgenic (WT) line. Bar = 50 μm

- Supplemental Fig. 4 H2O2 content and O2 production rate of transgenic (T) and non-transgenic (WT) pepper plants under both normal (‘control’) and 6 d drought stress treatments. Values are mean ± SD of three independent experiments (** p < 0.01).