1. Why Do We Use Gel Electrophoresis?
Total Page：16
File Type：pdf, Size：1020Kb
Name:______
Virtual Labs
Gel Electrophoresis: Go to: http://learn.genetics.utah.edu/content/labs/gel/ Click you way through the virtual lab and answer the questions below as you go.
1. Why do we use gel electrophoresis?
2. Where do you place the DNA sample before you run your gel?
3. How do you make DNA move through the gel?
4. How does the length of the DNA affect how the strands move through the gel?
5. How do you make DNA visible to the naked eye?
6. What do you need to make a gel?
7. What is agarose powder made of?
8. What is a buffer?
9. Why must you use a comb when making a gel?
10. What do you need to load a DNA sample into a gel?
11. Why should you use a loading buffer?
12. Why do you need to use the DNA size standard?
13. How do you know if your current is running?
14. What is the staining solution and why do we use it?
15. Draw your gel below. Be sure to indicate the approximate size of each band.
Click on “Can DNA Demand a Verdict” below when you are finished
1. What must a forensic scientist due in order to match the DNA Evidence to a suspect? 2. How is using DNA in forensics different now than how it use to be? (Hint: amount)
3. How do forensic scientists test for possible DNA contamination?
4. IS DNA evidence all you need to solve a case?
Go to: http://learn.genetics.utah.edu/content/labs/pcr/ Click “Begin” and work your way through the virtual lab, answering the questions below as you go.
1. What does PCR stand for?
2. Why do we use PCR?
3. What do you need to perform PCR?
4. What does a primer do?
5. Why do you need to add nucleotides?
6. Why do you need to add DNA polymerase?
7. What piece of machinery do we place the PCR tube in?______
8. Describe what happens in cycle 1 and 2.
9. Describe what happens in cycle 3.
10. Approximately how many cycles does PCR go through?______Go to: http://www.pbs.org/wgbh/nova/education/body/create-dna-fingerprint.html Click “View” and work your way the virtual lab.
1. What is the scientific name of the procedure you just did?______
2. Who is the culprit?______
Go to: http://www.biotechnologyonline.gov.au/popups/int_dnaprofiling.html Click “Continue”. You must investigate BOTH scenarios and answer the questions below.
Investigate the robbery
1. Who does the blood sample from the crime scene match?______
2. Who does the hair sample from the crime scene match?______
3. Was the suspect at the crime scene?______
Investigate the will
4. How many bands do Percy and Percy Jr. have in common?______
5. How many bands do Harriet and Percy Jr. have in common?______
6. How many bands does Alistair have in common with Percy?______
7. How many bands does Ann have in common with Percy?______
8. How many bands does Alistair have in common with Harriet?______
9. How many bands does Ann have in common with Harriet?______
10. Which children is Percy the father of?______
11. Which children is Harriet the mother of?______
12. Should Alistair receive some of Percy’s estate? Why?