<p>Name:______</p><p>Virtual Labs</p><p>Gel Electrophoresis: Go to: http://learn.genetics.utah.edu/content/labs/gel/ Click you way through the virtual lab and answer the questions below as you go.</p><p>1. Why do we use gel electrophoresis?</p><p>2. Where do you place the DNA sample before you run your gel?</p><p>3. How do you make DNA move through the gel?</p><p>4. How does the length of the DNA affect how the strands move through the gel?</p><p>5. How do you make DNA visible to the naked eye?</p><p>6. What do you need to make a gel?</p><p>7. What is agarose powder made of?</p><p>8. What is a buffer?</p><p>9. Why must you use a comb when making a gel?</p><p>10. What do you need to load a DNA sample into a gel?</p><p>11. Why should you use a loading buffer?</p><p>12. Why do you need to use the DNA size standard?</p><p>13. How do you know if your current is running?</p><p>14. What is the staining solution and why do we use it?</p><p>15. Draw your gel below. Be sure to indicate the approximate size of each band.</p><p>Click on “Can DNA Demand a Verdict” below when you are finished</p><p>1. What must a forensic scientist due in order to match the DNA Evidence to a suspect? 2. How is using DNA in forensics different now than how it use to be? (Hint: amount)</p><p>3. How do forensic scientists test for possible DNA contamination?</p><p>4. IS DNA evidence all you need to solve a case?</p><p>Go to: http://learn.genetics.utah.edu/content/labs/pcr/ Click “Begin” and work your way through the virtual lab, answering the questions below as you go.</p><p>1. What does PCR stand for?</p><p>2. Why do we use PCR?</p><p>3. What do you need to perform PCR?</p><p>4. What does a primer do?</p><p>5. Why do you need to add nucleotides?</p><p>6. Why do you need to add DNA polymerase?</p><p>7. What piece of machinery do we place the PCR tube in?______</p><p>8. Describe what happens in cycle 1 and 2.</p><p>9. Describe what happens in cycle 3.</p><p>10. Approximately how many cycles does PCR go through?______Go to: http://www.pbs.org/wgbh/nova/education/body/create-dna-fingerprint.html Click “View” and work your way the virtual lab.</p><p>1. What is the scientific name of the procedure you just did?______</p><p>2. Who is the culprit?______</p><p>Go to: http://www.biotechnologyonline.gov.au/popups/int_dnaprofiling.html Click “Continue”. You must investigate BOTH scenarios and answer the questions below.</p><p>Investigate the robbery</p><p>1. Who does the blood sample from the crime scene match?______</p><p>2. Who does the hair sample from the crime scene match?______</p><p>3. Was the suspect at the crime scene?______</p><p>Investigate the will</p><p>4. How many bands do Percy and Percy Jr. have in common?______</p><p>5. How many bands do Harriet and Percy Jr. have in common?______</p><p>6. How many bands does Alistair have in common with Percy?______</p><p>7. How many bands does Ann have in common with Percy?______</p><p>8. How many bands does Alistair have in common with Harriet?______</p><p>9. How many bands does Ann have in common with Harriet?______</p><p>10. Which children is Percy the father of?______</p><p>11. Which children is Harriet the mother of?______</p><p>12. Should Alistair receive some of Percy’s estate? Why?</p>
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