2016A Wet Mount Challenge Instructions

Dear Site Coordinator: 3/22/2016

The 2016A Wet Mount Proficiency Challenge is available in an electronic test format at the MDHH Regional Laboratory website. The images that comprise this challenge have been placed onto the Regional Laboratory Website in a Powerpoint Presentation designated “2016AWet Mount Proficiency”. Access this challenge by using the following hyperlink: (http://www.michigan.gov/mdch/0,1607,7-132-2945_5103_7168-74548--,00.html). The 2016A score sheet is available as a Word document at this same website. In order to complete this challenge, please follow the steps described below. Instructions for performing the PT challenge may also be downloaded from this website.

Prepare to perform the PT Challenge 1. Go to the MDHH website. 2. Download the 2016A Wet Mount Proficiency PowerPoint presentation and save it to your computer. 3. Download the 2016A Wet Mount Proficiency Instructions and Score Sheet (Word document) and print off a hard copy.

Performance of the PT Challenge 1. Complete the site specific information at the top of the score sheet. 2. Open the PowerPoint presentation. 3. There will be ten different items to evaluate (indicated by an arrow). 4. Evaluate each slide as if it comes from a different patient. Assume that this image is characteristic of findings from multiple fields. Some items may be shown with both low and high power objectives. An individual slide may contain more than one item to identify. 5. Identify the cellular element marked by the arrow on each slide. 6. Write your one, best answer on the score sheet. A list of possible responses is listed on the score sheet. Multiple answers for a single item are unacceptable and will be marked wrong. 7. Each individual performing this PT challenge must perform this challenge independently and without consultation with other staff members. Staff may discuss their results with others only after the results have been submitted to their laboratory director or designee.

Submission and grading of result forms 1. The person performing the proficiency test must sign and date the report form as the “testing person”. 2. The laboratory director or designee must sign and date the report form in the appropriate place. 3. Testing must be completed within 10 working days of receipt of the electronic notification of the availability of this challenge. 4. Unlike previous challenges, your laboratory director or site coordinator will grade your responses. The expected results will be provided to each agency after 4/22/2016

Questions?? Contact Stephen Haskell, coordinator of the Michigan Regional Laboratory System Wet Mount Proficiency Program, at 517-335-8134 ([email protected]) with any questions. Wet Mount Proficiency Test 2016A Agency: Clinic: Participant Date

Use this score sheet to document your evaluation of the wet mount challenge found at the following web site: http://www.michigan.gov/mdch/0,1607,7-132-2945_5103_7168-74548--,00.html Evaluate each micrographs as if they are typical findings found during a wet mount analysis. Identify each numbered item from the list of potential answers and write in the correct response. Indicate the one, best answer. Multiple answers are not acceptable and will be marked wrong. Potential Answers Clue Cell Trichomonas Squamous epithelial cell Yeast cell Artifact Pseudohyphae Red Blood cell Sperm cell(s) Bacteria White blood cell(s)

Item #1

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Attestation Statement: I, the undersigned, have analyzed these micrographs using the same criteria used in the analysis of regular patient specimens. I acknowledge that I have not collaborated with other staff members from my clinic or any other clinic in the analysis of these micrographs. I recognize that the use of micrographs does not accurately reflect the manner in which wet mount analysis is routinely performed.

Testing Person: ______Date: ______

Supervisor Review I, the undersigned, acknowledge that these micrographs were evaluated by this testing person in a manner consistent with the requirements of the Regional Laboratory system. The results reported here are the findings of this individual and were not obtained in collaboration with other staff members at this clinic or any other clinic.

Laboratory Director/Designee: ______Date: ______