Fluid Optimization Guided by Bioreactance-Based Passive Leg Raise Test

Electronic supplement material 2

Table 2b. Hemodynamic values during minutes 1to 5 of the PLR.

baseline 1 2 3 4 5

CO (L.min-1) 4.17±1.04 4.34±1.17 4.39±1.14 4.39±1.13 4.37±1.16 4.37±1.14 SV (mL) 49±12 50±15 51±16 51±16 51±16 51±17 HR (beat.min-1) 88±18 88±20 87±19 87±19 87±19 87±19

HR = heart rate, 3

Table 2c. Magnitude of FR % change as compared to PLR% change

All PLR≥0% PLR>4% PLR>9% PLR>14%

N of patients 75 53 33 23 12 %13-15’FR/PLR 1.60 1,36 1.17 1.00 0.86 %End FR 3.02 2,14 1.64 1.37 1.05

When PLR-induced % change in CO is low, FR is superior to PLR-induced CO change but the proportional changes tended to be equivalent when PLR -induced % change in CO increases. 4

Table 3b. Predictability at different moments of PLR for FR.

PLR >4% for FR >9% PLR >9% for FR >9%

Se Sp CC Se SP CC

PLR 2min 0.83 0.84 0.68 0.55 0.93 0.52 PLR 3min 0.86 0.76 0.63 0.65 0.84 0.50 PLR 4min 0.85 0.79 0.63 0.61 0.86 0.48 PLR 5min 0.86 0.82 0.68 0.57 0.87 0.46 PLR 2-4 min 0.89 0.79 0.68 0.65 0.92 0.59 PLR 3-5 min 0.89 0.84 0.73 0.68 0.95 0.65 5

Table 3c. Predictability of PLR for FR

PLR ≥0% PLR >4% PLR >9% PLR >14%

Fluid ≥0% - (-/0,1) - (-/0.3) - (-/0.5) - (-/0,8) Fluid >4% 44 (4,4/0,1) 73 (18/0.25) - (-/0 5) - (-/0.8) Fluid >9% - ((2.7/-) 44 (5.7/0.1) 38 (13/0.3) - (-/0.7) Fluid >14% - (1 9/-) 22 (2,8/0.1) 5 (2.6/0.5) 2 (2.1/0.9) Fluid >19% - (1.6/-) 13 (2.1/0.2) 3 (1.8/0/6) 1.4 (1.3/0.9)

Odd ratio (Positive likelihood ratio/negative likelihood ratio) values for the same pair of thresholds than shown in Table 3. “–“ indicates that the ratio is not derivable. 6

Responders Heart Rate (bpm) Nonresponders

70 Stroke Volume (ml) * 80

30 Cardiac Output (L/min)

PLR Fluid Bolus Baseline 1 Baseline 2

Figure 4. This figure summarizes changes in heart rate, stroke volume and cardiac output at baseline, at the end of the PLR test, during the second baseline period and then at the end of the fluid bolus, comparing responders and nonresponders.