Table S1: Origin and Characteristics of Soybean Rhizobia

Diversity and Phylogeny of Soybean Rhizobia in Central India; Parveen G ANSARI, D. L. N. RAO and K.K.PAL, Indian Institute of Soil Science, Bhopal, India; [email protected]

Table S1: Origin and Characteristics of Soybean Rhizobia Strain Origin Coordinates* Colony EPS Colony Growth Acid/alkali Growth in Reduction Litmus Size morphology Rate production Hoffers of BCP milk test (mm) in alkaline in GPA CRYEMA broth 1 R1 Bhopal 23 18.46’ N 77 24.47’ E 10 - Pink M Acid Yes No Acid 2 R2 Rajgarh 23 30.22’ N 77 03.86’ E 2 + White opaque S Acid No No Acid 3 R3 Anuppur 22 48.54’ N 81 43.38’ E 8 +++ Watery F Acid No No Acid translucent 4 R4 Betul 21 51.56’ N 77 56.25’ E 6 +++ Watery F Acid Yes No Acid translucent 5 R5 Betul 21 51.72’ N 77 56.75’ E 4 ++ Watery F Acid Yes No Acid translucent 6 R6 Chhattarpur 24 53.43’ N 79 54.66’ E 2 + Pink M Alkali Yes Yes Acid 7 R7 Chhattarpur 24 51.09’ N 79 39.30’ E 3 - Pink with M Acid Yes No Acid yellow center 8 R8 Chhindwara 22 01.79’ N 78 55.91’ E 5 +++ Watery F Acid Yes Yes Acid translucent 9 R9 Dewas 23 05.94’ N 76 13.04’ E 5 ++ Watery F Acid Yes Yes Acid translucent 10 R10 Dhar 22 34.51’ N 75 20.94’ E 5 ++ Watery F Alkali Yes Yes Acid translucent 11 R11 Dhar 22 34.42’ N 75 20.70’ 6 ++ Watery F Acid Yes No Acid N translucent 12 R12 Harda 22 22.38’ N 77 04.48’ E 5 ++ Watery F Acid Yes No Acid translucent 13 R13 Indore 22 49.26’ N 75 38.03’ E 5 - Pink M Acid Yes No Alkali 14 R14 Narsinghpur 23 02.27’ N 79 01.07’ E 4 ++ Watery F Acid Yes No Acid translucent 15 R15 Rajgarh 23 30.22’ N 77 03.86’ E 1 + White opaque S Acid No No Alkali 16 R16 Rajgarh 23 30.22’ N 77 03.86’ E 1 + White opaque S Alkali No No Alkali 17 R17 Rajgarh 23 30.22’ N 77 03.86’ E 1 ++ White opaque S Alkali No No Alkali 18 R18 Seoni 22 36.78’ N 79 37.24’ E 5 +++ Watery F Acid No No Alkali translucent 19 R19 Tikamgarh 24 43.29’ N 78 49.28’ E 4 + White opaque S Acid Yes Yes Acid 20 R20 Vidisha 23 30.07’ N 77 46.17’ E 5 +++ Watery F Alkali Yes No Acid translucent 21 R21 Dhar 22 41.20’ N 75 37.48’ E 2 + Pink M Alkali Yes No Acid (Indore 1a) 22 R22 Dhar 22 41.14’ N 75 37.47’ E 3 + Water F Acid Yes No Acid (Indore 3c) translucent 23 R23 Dhar 22 37.10’ N 75 22.66’ E 2 + Watery F Acid Yes Yes Acid (Indore 5a) translucent 24 R24 Sehore 22 57.03’ N 77 66.99’ E 3 + Watery F Acid Yes Yes Acid (Indore 7.1) translucent 25 R25 Sehore 22 58.68’ N 77 04.89’ E 5 + Milky F Acid Yes Yes Acid (Indore 7a) 26 R26 Sehore 23 72.53’ N 76 54.47’ E + White opaque S Alkali Yes Yes Acid (Indore 9b) 27 R27 USDA-110-BJ - 5 + Water S Acid Yes Yes Acid translucent 28 R28 USDA-122-BJ - 2 + White opaque S Acid Yes No Acid 29 R29 USDA-31-BE - 5 + Water S Acid Yes Yes Acid translucent 30 R30 Liphatech - 1 + White opaque S Alkali Yes No Alkali isolate 31 R31 Liphatech - 1 + White opaque S Alkali Yes No Alkali isolate 32 R32 Liphatech- - 1 + White opaque S Alkali Yes No Alkali reisolate 33 R33 Vidisha 23 29.79’ N 77 45.83’ E 1 + White opaque S Alkali No No Alkali 34 R34 Vidisha 23 29.79’ N 77 45.83’ E 1 + White opaque S Alkali Yes No Acid 35 R35 Vidisha 23 29.79’ N 77 45.83’ E 1 + White opaque S Alkali Yes No Alkali 36 R36 Vidisha 23 29.79’ N 77 45.83’ E 1 + White opaque S Alkali No No Alkali 37 R50 Parbhani 19 15.27’ N 76 46.40’ E 2 + White opaque S Alkali No No Acid 38 R51 Pantnagar 29 01.32’ N 79 28.96’ E 2 + White opaque S Alkali No No Acid 39 R52 Palampur 32 60.76’ N 76 32.47’ E 2 + White opaque S Alkali Yes No Alkali 40 R53 Ludhiana 30 53.87’ N 75 48.36’ E 2 + White opaque S Alkali Yes No Alkali * The coordinates denote the location of the villages/institute from where soils were sampled. S=slow grower M=medium grower F=Fast grower; CRYEMA= Congo-red yeast extract mannitol agar; EPS=Extra-polysaccharide; BCP= Bromocresol purple; GPA= Glucose peptone agar. All strains are negative for ketolactose test. The soybean isolates (R1-R2, R32-R36) are from IISS culture collection- R-3 to R-20 from the AICRP-BNF survey collection at JNKVV, Jabalpur; R21-26 from Directorate of Soybean Research, Indore (codes are given in parenthesis) ; R30-31 were isolated from a commercial liquid inoculant; R 32 is a re-isolate from commercial inoculant after plant passage, R50-53 are soil isolates.