LSR II Fluorochromes

Lasers 488 nm 100 mW Sapphire 635 nm 20 mW HeNe 406 nm 40 mW VioFlame

Filter Sets

VioFlame Laser – 406 nm LP Det. Filter 505 585/42 564-606 nm Pacific Orange --- 450/50 425-475 nm Cascade Blue, DAPI, Pacific Blue, Hoechst, Alexa Flour 405, Live/Dead Violet

HeNe Laser – 635 nm LP Det. Filter 755 780/60 750-810 nm APC-Cy7 685 710/50 685-735 nm APC-Cy5.5 --- 660/20 650-670 nm APC

Sapphire Laser – 488 nm LP Det. Filter 755 780/60 750-810 nm PE-Cy7 635 670/14 663-677 nm PE-Cy5, TriColor, CyChrome 595 610/20 600-620 nm PE-Texas Red, ECD 550 575/26 562-588 nm PE 505 530/30 515-545 nm FITC, AF 488 --- 488/5 485-490 nm Side Scatter

Additional Filter Sets LP Det. Filter 690 710/50 685-735 nm Cy5.5 600 610/20 600-620 nm Texas Red 525/50 500-550 nm Alexa Fluor 430 (bad), Cascade Yellow, Live/Dead Aqua

D:\Docs\2017-12-28\0b6933dc09b0aa0276085e9a2e113a46.doc Fluorochromes

Peak(nm) Excitation Emission

488 Sapphire Laser FITC, Alexa Fluor 488 495 520 R-PE (R-Phycoerythrin) 496, 565 575, 580 PE-TR (PE-Texas Red) 495 620 PE-Cy5 (CyChrome, Tricolor) 496 667 PerCP 482 675 PE-Cy5.5, PerCP-CY5.5 490 692 PE-Cy7 495 755, 778 PI (propidium ) 535 (broad) 620 (broad) 7-AAD (7-AminoActinomycin D) 545 (broad) 645 (broad)

635 HeNe Laser APC (Allophycocyanin) 620, 645 655 APC-Cy5.5 620 692 APC-Cy7 620 755, 778

Cy5 650 667 Alexa Fluor 647 650 668

406 VioFlame Laser Alexa Fluor 405 401 421 Alexa Fluor 430 AMCA 350 450 DAPI Cascade Blue 377 420 Pacific Blue 377 420 Pacific Orange 551 Hoechst

Can Not Be Used Cy3 550 570 TRITC 550 590 Rhodamine Red 570 590 Texas Red 596 620 Alexa Fluor 700 696 719 Acridine Orange 500 525

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