<p> LSR II Fluorochromes</p><p>Lasers 488 nm 100 mW Sapphire 635 nm 20 mW HeNe 406 nm 40 mW VioFlame</p><p>Filter Sets</p><p>VioFlame Laser – 406 nm LP Det. Filter 505 585/42 564-606 nm Pacific Orange --- 450/50 425-475 nm Cascade Blue, DAPI, Pacific Blue, Hoechst, Alexa Flour 405, Live/Dead Violet</p><p>HeNe Laser – 635 nm LP Det. Filter 755 780/60 750-810 nm APC-Cy7 685 710/50 685-735 nm APC-Cy5.5 --- 660/20 650-670 nm APC</p><p>Sapphire Laser – 488 nm LP Det. Filter 755 780/60 750-810 nm PE-Cy7 635 670/14 663-677 nm PE-Cy5, TriColor, CyChrome 595 610/20 600-620 nm PE-Texas Red, ECD 550 575/26 562-588 nm PE 505 530/30 515-545 nm FITC, AF 488 --- 488/5 485-490 nm Side Scatter</p><p>Additional Filter Sets LP Det. Filter 690 710/50 685-735 nm Cy5.5 600 610/20 600-620 nm Texas Red 525/50 500-550 nm Alexa Fluor 430 (bad), Cascade Yellow, Live/Dead Aqua</p><p>D:\Docs\2017-12-28\0b6933dc09b0aa0276085e9a2e113a46.doc Fluorochromes</p><p>Peak(nm) Excitation Emission</p><p>488 Sapphire Laser FITC, Alexa Fluor 488 495 520 R-PE (R-Phycoerythrin) 496, 565 575, 580 PE-TR (PE-Texas Red) 495 620 PE-Cy5 (CyChrome, Tricolor) 496 667 PerCP 482 675 PE-Cy5.5, PerCP-CY5.5 490 692 PE-Cy7 495 755, 778 PI (propidium ) 535 (broad) 620 (broad) 7-AAD (7-AminoActinomycin D) 545 (broad) 645 (broad)</p><p>635 HeNe Laser APC (Allophycocyanin) 620, 645 655 APC-Cy5.5 620 692 APC-Cy7 620 755, 778</p><p>Cy5 650 667 Alexa Fluor 647 650 668</p><p>406 VioFlame Laser Alexa Fluor 405 401 421 Alexa Fluor 430 AMCA 350 450 DAPI Cascade Blue 377 420 Pacific Blue 377 420 Pacific Orange 551 Hoechst</p><p>Can Not Be Used Cy3 550 570 TRITC 550 590 Rhodamine Red 570 590 Texas Red 596 620 Alexa Fluor 700 696 719 Acridine Orange 500 525</p><p>D:\Docs\2017-12-28\0b6933dc09b0aa0276085e9a2e113a46.doc</p>