Data Supplement For

Data supplement for

Excess copper induces anoxygenic photosynthesis in Anabaena doliolum: a homology based proteomic assessment of its survival strategy

Poonam Bhargava, Yogesh Mishra, Ashish Kumar Srivastava, Om Prakash

Narayan, Lal Chand Rai*

From the

Molecular Biology Section, Laboratory of Algal Biology,

Department of Botany, Banaras Hindu University,

Varanasi-221005,

1 Figure 1 supplement. MALDI TOF MS/MS spectrum of transketolase tryptic peptide

Figure 2 supplement. MALDI TOF MS/MS spectrum of Phycoerythrocyanin α- chain tryptic peptide

Figure 3 supplement. MALDI TOF MS/MS spectrum of Iron superoxide dismutase tryptic peptide

Figure 4 supplement. MALDI TOF MS/MS spectrum of Hypothetical protein alr

0803 tryptic peptide

Figure 5 supplement. MALDI TOF MS/MS spectrum of Manganese superoxide dismutase tryptic peptide

Figure 6 supplement. MALDI TOF MS/MS spectrum of Phosphoribulokinase tryptic peptide

Figure 7 supplement. MALDI TOF MS/MS spectrum of Plastocyanin tryptic peptide

2 Figure 1 supplement

7 Mascot Search Results User : Email : Search title : BHU-3-LCMS MS data file : \\Chemstation01\Data\BHU26\BHU 3 26 MAY-F.mgf Database : MSDB 20050227 (1942918 sequences; 629040812 residues) Taxonomy : Bacteria (Eubacteria) (759576 sequences) Timestamp : 26 May 2006 at 11:05:08 GMT Significant hits: AD2321 phosphoribulokinase [imported] - Nostoc sp. (strain PCC 7120)

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 47 indicate identity or extensive homology (p<0.05). Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

1. AD2321 Mass: 38659 Score: 111 Queries matched: 6 phosphoribulokinase [imported] - Nostoc sp. (strain PCC 7120) Check to include this hit in error tolerant search or archive report Query Observed Mr(expt) Mr(calc) Delta Miss Score Expect Rank Peptide 44 625.65 1249.29 1249.63 -0.34 0 38 0.61 1 R.YEDVLAAINSR.K 50 533.65 1597.93 1597.86 0.07 0 (16) 87 3 K.YQGELTQLLLQHR.E 51 533.82 1598.44 1597.86 0.58 0 19 40 3 K.YQGELTQLLLQHR.E 58 899.86 1797.71 1798.02 -0.32 0 (21) 24 1 R.EFADVVLQVLPTNLIK.D 59 600.25 1797.73 1798.02 -0.30 0 36 0.77 1 R.EFADVVLQVLPTNLIK.D 61 1015.23 2028.45 2028.00 0.45 0 22 18 1 R.EYPGSNNGTGFFQVLTGLK.M Figure 6 supplement

8 Mascot Search Results User : Email : Search title : BHU-S1-LCMS MS data file : \\Chemstation01\Data\BHU26\BHU S1 26 MAY-3.mgf Database : MSDB 20050227 (1942918 sequences; 629040812 residues) Taxonomy : Bacteria (Eubacteria) (759576 sequences) Timestamp : 26 May 2006 at 11:07:10 GMT Significant hits: CUAI plastocyanin - Anabaena variabilis

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 48 indicate identity or extensive homology (p<0.05). Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

1. CUAI Mass: 11154 Score: 78 Queries matched: 2 plastocyanin - Anabaena variabilis Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) Delta Miss Score Expect Rank Peptide 25 487.29 972.57 972.56 0.00 0 36 1.3 1 K.GLLVFEPAK.L 50 563.64 1687.90 1687.90 -0.01 0 44 0.12 1 K.VPPHNVVFDAALNPAK.S

Proteins matching the same set of peptides: AB1839 Mass: 14667 Score: 78 Queries matched: 2 plastocyanin precursor [imported] - Nostoc sp. (strain PCC 7120) Figure 7 supplement