Hoagland Nutrient Solution

Hoagland Nutrient Solution t r a n t

Compound l Conc. of Conc. of Volume of Final conc. Final conc. e h u g c m

1 i stock stock stock of element of element e e e l l o E

w solution solution solution pre ppm

M (M) g / liter liter of final solution (ml)

KHO3 101.10 1.00 101.10 6.0 N 16000 224

Ca(NO3)2. 4 236.16 1.00 236.16 4.0 N 6000 235 H2O

(NH4)2PO4 115.08 1.00 115.08 2.0 Ca 4000 160

MgSO4. 246.49 1.00 246.49 1.0 P 2000 62 7 H2O Compound 2 (mM) KCl 74.55 50 3.728 Cl 50 1.77

H3BO3 61.84 25 1.516 B 25 0.27

MnSO4 . 169.01 2.0 0.338 Mn 2.0 0.11 H2O

ZnSO4. 289.55 2 0.575 Zn 2 0.131 7H2O 1 Liter

CuSO4 . 249.71 0.5 0.125 Cu 0.3 0.032 5H2O

H2MoO4 161.97 0.5 0.081 Mo 0.5 0.05 (85% MoO3) Fe-EDTA3 346.08 20 6.922 1 Liter Fe 20 1.12 1. After Johnson et. al. (1957), modified. 2. A combined stock solution is made up containing all micronutrients except iron. 3. Ferrous dihydrogen ethyleamine tetra acetic acie. For M.Sc Botany 3 rd semester; BS Botany 7 th semester

Growth responses of maize, sunflower and cotton varying nitrogen regimes

Modified Hoagland’s nutrient solution for varying nitrogen regimes 4, 8, 12, 16 and 20mM N

Sr.No Varying N (mol m-3) in Hoagland’s nutrient solution 4 8 12 16 20 -1 -1 -1 -1 KNO3 1.5mL 3mL L 4.5mL L 6 mL L 6.5 mL L -1 -1 -1 -1 -1 Ca(NO3)2.4H2O 1 mL L 2 mL L 3 mL L 4 mL L 5 mL L -1 -1 -1 -1 NH4 H2PO4 0.5mL 1 mL L 1.5mL L 2mL L 2.5 mL L -1 -1 -1 -1 -1 MgSO4 1mL L 1 mL L 1 mL L 1 mL L 1 mL L K2HPO4 2.25 mL L-1 1.5 mL L-1 0.75 mL L-1 - - -1 -1 -1 CaCl2.2H2O 3mL L 1.5 mL L 0.75 mL L - - Fe-EDTA 1mL L-1 1mL L-1 1mL L-1 1mL L-1 1mL L-1 Micronutrients 1mL L-1 1mL L-1 1mL L-1 1mL L-1 1mL L-1

Mean values for …………………….. of maize/sunflower/cotton when 21 day-old plants were subjected for varying concentrations of nitrogen in sand culture. Sr No Desi Bhindi Varying N (mol m-3) in Hoagland’s nutrient solution

Rep.No 4 8 12 16 20 1 2 3 4 5 Mean Std. Dev. S.E

Analysis of variance of data for …………………….. of maize/sunflower/cotton when 21 day-old plants were subjected for varying concentrations of nitrogen in sand culture.

SOV df SOS MS F value P Value

Nitrogen

Error/Residual

Cultivars 4mM N 8 mM N 12 mM N 16 mM N 20 mM N

LSD = Effect of varying levels of N on growth of maize, sunflower, cotton Use following treatments

-1 -1 -1 T1 = N 56 mg L = 1.5mL 1M KNO3 L + 1mL 1M Ca (NO3)2 .2H2O + 0.5mL 1M NH4H2PO4 L + 2.25

mL 1M K2HPO4 + 3mL 1M CaCl2.2H2O + 1mL 1M Mg SO4 + 1mL Micronutrients + 1mL Fe-

-1 -1 -1 T2 = N 112 mg L = 3mL 1M KNO3 L + 2mL 1M Ca (NO3)2 .2H2O + 1mL 1M NH4H2PO4 L + 1.5 mL

1M K2HPO4 + 1.5mL 1M CaCl2.2H2O + 1mL 1M Mg SO4 + 1mL Micronutrients + 1mL Fe-

-1 -1 -1 T3 = N 168 mg L = 4.5mL 1M KNO3 L + 3mL 1M Ca (NO3)2 .2H2O + 1.5mL 1M NH4H2PO4 L

+0.75mL 1M K2HPO4 + 0.75mL 1M CaCl2.2H2O + 1mL 1M Mg SO4 + 1mL Micronutrients + 1

mL Fe-EDTA

-1 -1 -1 T4 = N 224 mg L = 6 mL 1M KNO3 L + 4 mL 1M Ca (NO3)2 .2H2O + 2 mL 1M NH4H2PO4 L + 1mL

1M Mg SO4 + 1mL Micronutrients + 1 mL Fe-EDTA