Table 1 Samples of Boletus Edulis investigated in this study Sample 1 Harvest 2006 year Pine forest from Rodopi First grade Sample 2 Harvest 2007 year Pine forest from Rodopi First grade Sample 3 Harvest 2007 year Pine forest from Rodopi Second grade Sample 4 Harvest 2008 year Pine forest from Rodopi Third grade Sample 5 Harvest 2008 year Deciduous trees forest in Sredna Gora Second grade

Table 2 Content of 11 chemical elements in dried Boletus Edulis in mg/kg Element Sample 1 Sample 2 Sample 3 Sample 4 Sample 5

X ± Δ X ± Δ X ± Δ X ± Δ X ± Δ Se 14.0 ± 0.7 19.3 ± 0.9 26.3 ± 1.1 29.6 ± 1.2 32.0 ± 1.1 Hg 1,0 ± 0,3 3,0 ± 0,8 4,5 ± 0,8 2.6 ± 0.5 3,8 ± 0,6 Al 13,2 ± 0,4 13,0 ± 0,6 50,0 ± 2,8 44.4 ± 1.9 31,0 ± 1,8 Cu 12,3 ± 0,6 22,0 ± 0,8 25,8 ± 1,4 27.1 ± 1.7 33,5 ± 2,5 Zn 62,8 ± 0,9 79,3 ± 2,2 95,8 ± 2,5 93.0 ± 2.6 26,0 ± 0,5 Cd 0,5 ± 0,2 0,8 ± 0,3 1,0 ± 0,3 1.25 ± 0.3 2,3 ± 0,5 Pb 0,5 ± 0,3 0,6 ± 0,3 0,5 ± 0,2 1.8 ± 0.9 0,9 ± 0,3 Mg 430 ± 8 650 ± 10 750 ± 12 870 ± 16 650 ± 10 Ca 160 ± 9 145 ± 8 210 ± 12 320 ± 15 130 ± 8 Fe 35 ± 3 33 ± 2 106 ± 6 54 ± 8 48 ± 5 As < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 * X mean value *Each mean value is from five replicate determinations within a confidence interval at  = 0.05

Table 3 Comparison of IR and MW mineralization. Sample 2 is analyzed (n =5;  = 0.05) Kind of mineralization Se mg/kg Hg мg/кg IR 18.7 ± 0.4 2.80 ± 0.6 MW 19.0 ± 0.4 2.85 ± 0.5

Table 4 Antioxidant activity in relative units. The results are averages from three replicate samples with relative standard deviation < 10% Product Boletus Edulis Lactarius Amanita Agaricus Garlic Leek Deliciosus Caesarea Bisporus Trolox 82000 56000 59000 75000 52000 53000 equivalent

Table 5 Boletus Edulis inhibits copper-induced lipid peroxidation (LPO) of phosphatidylcholine multilamellar vesicles. Lipids were incubated for 24 h at 37 ºC with CuSO4 in the absence or presence of the indicated concentrations of dried mushrooms. Lipids 0.191 Lipids/2 µM CuSO4 0.855 Lipids/5 µM CuSO4 1.114 Lipids/10 µM CuSO4 0.985 Blood serum/50 µM CuSO4 2.194 Lipids/5 µM CuSO4/ 0,25 mg/ml Boletus Edulis 0.455 Lipids/5 µM CuSO4/ 0,5 mg/ml Boletus Edulis 0.158

Lipids/5 µM CuSO4/ 1,0 mg/ml Boletus Edulis 0.353 Lipids/5 µM CuSO4/ 2,5 mg/ml Boletus Edulis 0.839 2,5 mg/ml Boletus Edulis 0.202