Program The 12th Transgenic Technology Meeting took place at The Assembly Rooms, 54 George Street, Edinburgh, EH2 2LR. Sunday October 5, 2014 10:00 - 13:00 ISTT Council Meeting 16:00 - 18:00 Registration open Pre-Meeting Social Event - Scottish Dinner and Ceilidh taster 18:00 - 22:00 Ghillie Dhu, 2 Rutland Place, Edinburgh, EH1 2AD

Monday October 6, 2014 07:30 - 20:00 Registration open Welcome Address 09:00 - 09:15 ISTT President & Organising Committee Session 1: Opening Keynote Lecture Chair: David Hume iPS technology, gene editing and disease research 09:15 - 10:00 Rudolf Jaenisch Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, USA 10:00 - 10:30 Tea/Coffee break, posters and exhibition Session 2: Embryonic Stem Cells Chair: Peter Hohenstein Rapid in vivo validation of cancer genes and drug targets using ESC-GEMMs of human cancer 10:30 - 11:00 Jos Jonkers The Netherlands Cancer Institute, Amsterdam, The Netherlands The International Knockout Mouse Consortium (IKMC) resource: Finishing the genome 11:00 - 11:30 William Skarnes Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK Embryonic stem cells: capture of the ground state 11:30 - 12:00 Austin Smith Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, UK Scottish Science Showcase Presentation Transcripting factor function in pluripotent cell states 12:00 - 12:15 Ian Chambers MRC Centre for Regenerative Medicine, Edinburgh, UK Session 3: Short Talks and Teasers Selected from Abstracts Chair: Benoit Kanzler Novel tools to discern the pluripotency gene network establishment 12:15 - 12:30 Eleni Chantzoura MRC Centre for Regenerative Medicine, Edinburgh UK Poster Teasers Selected from Abstracts 12:30 - 12:45 Efficient shipment of refrigerated mouse embryos across Europe Julia Fernández National Center of Biotechnology (CNB), CSIC, Madrid, Spain

Derivation of haploid ES cells from genetically modified mice of interest in cancer research Carmen Gómez Spanish National Cancer Research Centre (CNIO), Madrid, Spain LincRNA pint knockout mice display early aging-associated phenotypes Guochun Gong Regeneron Pharmaceuticals, Tarrytown, NY, USA

RepLiCre: Reporter-linked-cre transgenic mice for efficient use of conditional knockout mouse resources Channabasavaiah Gurumurthy University of Nebraska Medical Center, Omaha, USA A new method to pick targeted ES cell clones more efficiently Geert Michel Charité - Universitätsmedizin Berlin, Berlin, Germany 12:45 - 14:45 Lunch/Posters/Exhibition 13:00 - 13:40 Taconic Lunchtime Symposia 13:45 - 14:45 Poster Viewing Session 1

Session 4: Round Table Discussion: Sponsored by What does the Future of Our GA/transgenic facilities look like? Chair: James Bussell Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK Transgenic and Archiving Module of the Czech Centre for Phenogenomics - a young and one of the largest transgenic core facility in central Europe Inken Beck, Institute of Molecular Genetics of the ASCR, Prague, Czech Republic 14:45 - 16:15 Mouse production of GA animals in a large-scale production centre: What does the future hold? Brendan Doe, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK

Transgenic core facilities: Past, present and where do we go from here? Lynn Doglio, Feinberg School of Medicine, Northwestern University, Chicago, USA The future of the large scale animal facility with complex mouse and rat models Xin Rairdan, Genentech Inc., South San Francisco, California, USA Tea/Coffee break, posters and exhibition

16:15 - 16:45

Sponsored by Session 5: Large-Scale Phenotyping Chair: Ian Jackson High-throughput mutant mouse PHENOTYPING is a powerful tool to generate novel hypotheses 16:45 - 17:15 Jacqueline White Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK Large-scale resource development at the Jackson Laboratory: KOMP2 and beyond 17:15 - 17.45 Stephen Murray The Jackson Laboratory, Bar Harbor, Maine, USA Session 6: ISTT Prize Introduction to the 10th ISTT Prize for Outstanding Contributions to Transgenic Technologies 17:45 - 17:55 Lluis Montoliu National Center of Biotechnology, CSIC, Madrid, Spain; ISTT President Award Ceremony, with Alexandre Fraichard, CEO genOway 10th ISTT Prize for outstanding contributions to Transgenic Technologies (Sponsored by genOway) Manipulating the mouse embryo: From cells to genes to stem cells and back again 17:55 - 19:00 Janet Rossant The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada Reception & Lord Provost Welcome 19:00 - 20:00 Assembly Rooms, 54 George Street, Edinburgh, EH2 2LR

Tuesday October 7, 2014 08:00 - 18:45 Registration open

Session 7: Animal Biotechnology: Sponsored by Chair: Lluis Montoliu Transgenic Animals for Agriculture – Where in the world are we now? 08:45 - 09:15 James Murray University of California, Davis, California, USA Modelling human cancers in pig 09:15 - 09:45 Angelika Schnieke WZW Center of Life Science, Freising-Weihenstephan, Germany Scottish Science Showcase Presentation Transgenic chickens: New birds in the coop 09:45 - 10:00 Michael McGrew The Roslin Institute, Edinburgh, UK Generating transgenic chickens 10:00 - 10:15 Adrian Sherman The Roslin Institute, Edinburgh, UK 10:15 - 10:45 Tea/Coffee break, posters and exhibition Session 8: Rodent Models Chair: Douglas Strathdee Cancer Gene Discovery in the Mouse 10:45 - 11:15 David Adams Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK Transgenic rats: Catching up with the mouse 11:15 - 11:45 Kai Schönig Central Institute of Mental Health, Heidelberg University, Mannheim, Germany Scottish Science Showcase Presentation Temporally tractable systems for interrogating tumour progression in vivo 11:45 - 12:00 Daniel J Murphy The Roslin Institute & MRC IGMM, Edinburgh, UK

Session 9: Short Talks and Teasers Selected from Abstracts Chair: Boris Jerchow Evaluation of Cas9-RNA guided nuclease for high-throughput mutant mouse production in C57BL/6N 12:00 - 12:15 Lauryl M J Nutter The Hospital for Sick Children, Toronto, Canada Editing myostatin in livestock animals 12:15 - 12:30 Rachel Huddart The Roslin Institute, Edinburgh, UK Poster Teasers Selected from Abstracts 12:30 - 12:45 Ultrasound confirmation of Pregnancy in Genetically Modified Mice Reduces Resources While Enhancing Reliability Vida Asghari Genentech, Inc. South San Francisco, USA

Interspecific Recombination Between Orthologous Human and Mouse BAC Clones in E. coli: Exploring Scalable Humanization of Cancer-Relevant Genes in the Mouse Genome David Bergstrom The Jackson Laboratory, Bar Harbor, USA

Screening and genotyping highly complex rodent models created by CRISPR-Cas9 endonuclease J Colin Cox Genentech, Inc. South San Francisco, USA In vivo comparative analysis of 50 sgRNA for mouse knockin models: efficacy and off target activities Sandra Fontaniere genOway, Lyon, France

One-step Generation of Mice Carrying Gene-edited Alleles by the CRISPR/Cas-mediated Genome Engineering with High Efficiency Wenning Qin The Jackson Laboratory, Bar Harbor, USA 12:45 - 14:30 Lunch/Posters/Exhibition 13:00 - 13:40 The Jackson Laboratory Lunchtime Symposia 13:45 - 14:45 Poster Viewing Session 2

Session 10: New Technologies: Sponsored by Chair: Bruce Whitelaw Editing the rat genome using gene-specific nucleases 14:45 - 15:15 Ignacio Anegon Center for Research in Transplantation and Immunology, Nantes, France The amazing opportunities from integrating optoacoustic and transgenic technology 15:15 - 15:45 Vasilis Ntziachristos Technische Universität München, Munich, Germany Genome engineering combining CRISPR with the best of the rest 15:45 - 16:15 Francis Stewart Biotechnology Center of the TU Dresden, Germany 16:15 - 16:45 Tea/Coffee break, posters and exhibition 16:45 - 18:45 ISTT General Assembly Meeting Dinner at Our Dynamic Earth 19:30 - 23:55 Our Dynamic Earth, Holyrood Road, Edinburgh, EH8 8AS

Wednesday October 8, 2014 08:00 - 18:00 Registration open Session 11: Zebrafish models and transgenesis Chair: Robert Kelsh The Zebrafish Molecular Genetics Programme Training Toolbox 08:45 - 09:15 Stephen Ekker Mayo Clinic, Rochester, Minnesota, USA The Tol2 transposon technology in vertebrates 09:15 - 09:45 Koichi Kawakami National Institute of Genetics, Shizuoka, Japan Targeted Genome and Epigenome Editing Using CRISPR-Cas and TALE Technologies 09:45 - 10:15 Keith Joung Massachusetts General Hospital, Charlestown, MA, USA Scottish Science Showcase Presentation A novel WT1 response to notochord injury and vertebra development in zebrafish 10:15 - 10:30 Elizabeth Patton MRC Human Genetics Unit & MRC IGMM, University of Edinburgh, UK 10:30 - 11:00 Tea/Coffee break, posters and exhibition

Session 12: Animal Ethics and Welfare: Sponsored by Chair: Aimee Stablewski 11:00 - 11:30 Breeding the unknown- the benefits and issues of breeding new genetic combinations Sara Wells MRC Harwell, Oxfordshire, UK Surplus animals in transgenic breeding: Bred but not used! 11:30 - 12:00 Jan-Bas Prins Leiden University Medical Centre, The Netherlands Scottish Science Showcase Presentation Transgenic tools to reduce animal numbers 12:00 - 12:15 Peter Hohenstein The Roslin Institute & MRC IGMM, Edinburgh, UK Session 13: Short Talks and Teasers Selected from Abstracts Chair: Elizabeth Williams What does good practice look like in the transgenic technology field? 12:15 - 12:30 Nicola Osborne Royal Society for the Prevention of Cruelty to Animals, West Sussex, UK The impact of gut microbiota on phenotype following rederivation 12:30 - 12:45 Marina McCoy University of Missouri, USA Poster Teasers Selected from Abstracts A protocol for nonsurgical embryo transfer with improved pregnancy and birth rates over surgical implantation Rahmen Bin Ali The Netherlands Cancer Institute, Amsterdam, USA

Anti-‘flu RNA interference in the chicken: using precision genome engineering to hitch-hike on miRNA expression Arjun Challagulla CSIRO, Geelong, Australia

12:45 - 13:00 Effective and Efficient Zebrafish Sperm Archiving Victoria King National Institute for Medical Research, London, UK

Germ cell differentiation of bovine pluripotent Stem Cells (biPSC) Luis Malaver CSIRO, Geelong, Australia

Blastocyst genotyping – Refining cryopreservation quality control Stuart Newman Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK 13:00 - 15:00 Lunch/Posters/Exhibition 13:15 - 13:55 Charles River Lunchtime Symposia 14:00 - 15:00 Poster Viewing Session 3 Session 14: Imaging Chair: Jan Parker-Thornburg Imaging cellular dynamics and fate in the early mouse embryo 15:00 - 15:30 Anna-Katerina Hadjatonakis Sloan-Kettering Institute, New York, USA Tracking elusive cells using high-resolution lineage tracing 15:30- 16:00 Pawel Pelczar Institute of Laboratory Animal Science, Zürich, Switzerland 16:00 - 16:30 Tea/Coffee break, posters and exhibition Session 15: ISTT Young Investigator Award and Best Poster Awards Chairs: Lluis Montoliu and Douglas Strathdee Presentation of the 2nd ISTT Young Investigator Award 16:30 - 16:40 Changhong Pang CEO, inGenious Targeting Laboratory Short Lecture by the recipient of the 3rd ISTT Young Investigator Award

16:40 - 17:00 Development and Application of CRISPR-Cas9 for Genome Editing Feng Zhang Harvard & MIT, Cambridge, MA, USA Session 16: Closing Keynote Lecture Chairs: Lluis Montoliu and Douglas Strathdee Interdependence of cellular networks ensures scaling of cell types during cerebellum development 17:00 - 17:45 Alexandra Joyner Sloan-Kettering Institute, New York, USA Concluding Remarks Presentation of Diplomas for the ISTT Registration Awards The ISTT Best Poster Awards, sponsored by Charles River Laboratories International, Inc. with David Grass, Ph.D. (Senior Director of Gen Test Embryology services, Charles River) 17:45 - 18:00 Presentation of the TT2016 meeting Radislav Sedlacek Institute of Molecular Genetics, Prague, Czech Republic 18:00 End of TT2014 Meeting