Table 1. Heavy-chain (A) and light-chain (B) variable region amino acid sequences of recombinant human antibody Fabs reacting specifically with recombinant hMPV F protein.
(A)
Fab FR1 CDR1 FR2 CDR2 FR3 CDR3 FR4 AC31 MAQVKLLEQSGAGTRKPWASVNLSCKVSGVSLR SYDLH WMRQPPGKRLEWMG WMNAGIGNTKYSQNLQG RVTISRDSTRTQATRRCPCLTSDDTVVYYCGK ANVLLWVREVESI WGQGTLVTVSS AC59 MAQVKLLEQSGTEVKKPGASVRVSCKASGFTFT DEYIH WVRQAPGQGLEWMG WIDPKTGDTKYSQKLQG WVTMTRDTSISTVYMELSRLRSDDTALYYCAR ESAGHLDAFDI WGQGTLVIVSS AC69 MAQVKLLESGGGLAQPGGSLRLSCAASGFTFF MHGM WVRQAPGRGLEWVS GIRGSGDETYYADSVKG RFSISRDNSKNTVFLQMNRLRADDTAVYYCAK DWHRGDWYRWFDP WGQGTLVTVSS S AC83 MAQVKLLESGPGLVKPSETLSLTCTVSGDSIT NSNYE WDRIPQSPGKGLEWIG SIYHSGFTDYNPSLKS RVTMSVDTSKNQFSLKMSSVTAADTAVIYCAR RSSGHYDAFDV WGQGTKVTVSS ACN04 MAQVKLLESGPGLVKPSETLSLTCTVSGGSIS SYYWT WIRQPPGKGLEWIG YSYPSVSTKYNPSLKS GAVRASGLHDAYDI WGQGTLVTVSS RVSISTDTSKSQFSLKLTSVTAADTAVYYCAR 4 DS1 MAEVQLLESGPGLVKPLETLSLTCAVSGGSIT GYYW WFRQPPGKGLEWIA NIYYSGNIDYSPSLRN RITVSLDTSNNHASLKINRVTVADTATYYCAR GRPLGMGFDP WGQGTLVLVSS S DS6 MAEVQLLESGPGVVKPSETLSLTCTVSGGSIT GYYW WFRQTPGKGLEWIT NIYYSGNIDYSPSLKS RITVSLDTSNNLASLKLTSVTAADTAMYYCAR GRPLGMGFDP WGQGTLVIVSS S DS7 MAEVQLLESGGGLVQPGGSRRLSCAASGFTVS SSYMS WVRQTPGKGLEWIS VFYSGGTTYYADAVKG RFSISMDTSKNTLHLQMNSLRVEDTAIYYCAR VLSRASGMPDAFDI WGPGTMVTVSS Han01 MAEVQLLEQSGAEVKKPGAAVKVSCKASGYTFT TYALH WLRQAPGQRLEWMG WINAGNGNTEYSEKFQG RITLTRDMSANTAYLELTNLRSEDAALYYCAR DERSVVQYFDWYLQH WGQGTLVTVSS Han02 MAEVQLLESGGGLVRPGRSLELSCTASGFSFR DYAFT WVRQAPGKGLEWVG SIRRKAYGETTEYAASVKG RFTISRDDSKSIAYLQMNSLRSEDSAVYYCSR VLSVLDYYFAMDV WGQGTTVTVSY Han05 MALEQVQLVQSGAELEKPGASVKVSCKASGYTFT TYALH WVRQAPGQSLEWVG WIHPGNGNTELSQKFQG RVSFTRDTSASTAYMELRSLRSEDTALYYCAR DERSVVQHFDWFLEH WGQGTLVTVSS Han09 MAEVQLLESGGGLVEPGGSLRLSCAASGFTFS HYYFN WIRQAPGRGLEWVS YISSGGGGTIHYAESVKG RFTISRDNAKNSVYLQMNSLRTEDTAVYYCSR GQYWFASGTYRAIDDAS WGQGTMVTVSS DI Han10 MAEVQLLESGGGLVQPGRSLGLTCTTSGFTFR DYAMT WVRQAPGKGLEWVG SIRRKAYGGTTEYAASVKG RFTISRDDSKSIAYLQMNSLKTEDTGVYFCTR VLSVLDYYYGMDV WGQGTTVAVSS LL01 MAEVQLLEQSGAEVRKPGASVKVSCTASGYTFT TFPIH WLRQAPGQRLEWMG WINAGNGNTESSQKFQG RVTFTRDTSASTAYMELSSLTSEDTAVYYCAR DERSVVQHFDWFLEY WGQGTLVTVSS (B)
Fab FR1 CDR1 FR2 CDR2 FR3 CDR3 FR4 ACN044 () MAELQPPSVSVSPGQTARITC SGENLGKKYVC WYQRKPGQSPVLVMY EDSKRPS GIPERFSGSNSGTTATLTISGTQAMDEADYFC QVWVSSTEVL FGGGTKLSVL AC31 () MAELTAVPDTLSLSPGERATLC RASQSLTSSFLA WYQQKPGQAPRFLIY DASIRAT GVPDRFSGSGSGTDFTLTISRLEPEDFAVYYC QQYGSSPRT FGQGTKVEVK AC59 () MAELTQSPSSLSASVGDRVTITC RASQSISSYLN WYQQKPGKAPKLLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQSYSSIT FGPGTKVDIK AC69 () MAELPQPHSVSESPGKTVVISC TRSSGKIDSNYVQ WYRRRPGSSPTVVIF EDDQRPS GVPDRFSGSIDRSSNSASLTISGLMAEDEADYYC QSYDARYQV FGGGTKLTVL AC83 () MAELTQSPSSLSASVGDRVTITC RASQSISSYLN WYQQKPGKAPKLLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQSYSNLWT FGQGTKVEIK DS7 () ELALIQPASVSVSPGQTASITC SGDKLGDKYAS WYQQKPGQSPVLVIY QDSERPS GIPERFSGSNSGNTATLTISGTQAMDEADYYC QAWDSSTAV FGGGTTLTVL DS1 () ELTQPPSVSGTPGQRVTISC TGSSSNIGTPYDVH WYQQLPGTAPKLLIY GDTNRPS GVPDRFSGSKS GTSASLAITGLQAEDEGDYYC QSYDSSLSGWV FGGGTKLTVL DS6 () ELALTQPPSVSGSPGQSITISC TGTSSDVGGYNFVS WYQHHPGKAPKLMIY DVSLRPS GVSNRFSGSKSGNTASLTISGLQAEDEADYYC SSYTGSSTVL FGGGTKLTVL Han1 () ELVMTQSPSSLSASVGDTITLTC RASQGIGESLA WYQQSPGKAPKLLLS AASRLES GVPSRFSGS GSGSDYTLTINSLQPEDFAMYYC QQYFGTPLT FGGGTKLEIK Han2 () ELALTQPPSVSGSPGQSITIS CTGTSNDVGSSNLVS WYQQHPGKAPKLMIH EASKRPS GVSNRFSGSKSGNTASLTISGLQAEDEADYYC SSYAGSSTVV FGGGTKLTVL Han5 () ELTLTQSPGTLSLSPGERATLSC RASQSVGKSYLA WYQQKPGQAPRLLLY GSSNRAT GIPDRFSGSGSGTDFTLTINRLAPEDFAVYFC QQYGSSPAT FGQGTKVEIK Han9 () ELTQSPLSLPVTLGQPASISC RSSESLLYTDGNTYLN WFQQRPGQSPRRLLY QVSNRDP GVPDRFRGSGSGTDFTLRISRVEAEDVGTYYC MQATHWPPYT FGQGTKLEIN Han10 () ELVLTQPPSVSAAPGQKVTISC SGNSSNIESNYVS WYQQLSGRAPKLLIY DNNKRPS GIPERFSGSKSGTSATLGVTGLQPGDEADYYC ATWDNSLSAGV FGGGTRLTVL LL01 () ELVMTQSPSSLSQFVGGRVTITC RASQHIGNSLA WYQQSPGKAPKLLLS DTSKLQS GVPSRFSGSGSGPDYTLTINNVQPEDFGVYYC QQYFGTPLT FGGGTKLEIK