Curriculum Vitae s332

CURRICULUM VITAE

De-Yi Liu, Ph D, Scientific Director of Andrology Melbourne IVF 320 Victoria Parade, East Melbourne, VIC 3002, Australia http://www.mivf.com.au

Senior Research Fellow (honorary) University of Melbourne Department of Obstetrics & Gynecology, Royal Women's Hospital, Parkville VIC 3053, Australia.

Editorial Board Member and Reviewer: Associate Editor for the Human Reproduction from 2003 to 2006. Editorial board member for the Asia Journal of Andrology from 2003 to 2010 and the Journal of Reproduction and Contraception (Chinese) from 2004-2010.

Reviewer for the following scientific journals: Human Reproduction, Fertility and Sterility, Human Reproduction Update, International Journal of Andrology, Asia Journal of Andrology, Reproductive Biomedicine Online and Human Fertility.

Main research area: human sperm function and male infertility in the following fields: Semen analysis and new sperm function test. Sperm-oocyte interaction (zona-binding and penetration and zona-induced AR). Sperm capacitation and acrosome reaction. Sperm preparation for clinical ART. Sperm cryopreservation.

Publications (journal articles) 6. Liu DY, Clarke GN and Baker HWG. The effect of serum on motility of human spermatozoa in culture. International Journal of Andrology 9:109-117, 1986 7. Liu DY, Cooper EJ and Baker HWG. Seminal transferrin: an index of Sertoli cell function: Is it of clinical value? Clinical Reproduction and Fertility 4:191-197, 1986 8. Liu DY, Jennings MG and Baker HWG. Correlation between defective motility (asthenospermia) and ATP reactivation of demembranated human spermatozoa. Journal of Andrology 8:349-353, 1987 - 1 - 9. Liu DY, Elton RA, Johnston WIH and Baker HWG. Spermatozoal nuclear chromatin decondensation in vitro. A test for sperm immaturity. Comparison with results of human in vitro fertilisation. Clinical Reproduction and Fertility 5:191-201, 1987 10. Liu DY, Du Plessis YP, Nayudu PL, Johnston WIH and Baker WHG. The use of in vitro fertilization to evaluate putative tests of human sperm function. Fertility and Sterility 49:272-277, 1988 11. Liu DY and Baker HWG. The proportion of sperm with poor morphology but normal intact acrosomes detected with Pisum Sativum agglutinin correlates with fertilization in vitro. Fertility and Sterility 50:288- 293, 1988 12. Liu DY, Lopata A, Johnston WIH and Baker HWG. A human sperm-zona pellucida binding test using oocytes that failed to fertilize in vitro. Fertility and Sterility 50:782-788, 1988 13. Liu DY, Lopata A, Johnston WIH and Baker HWG. Human sperm-zona pellucida binding, sperm characteristics and in vitro fertilization. Human Reproduction 4:696-701, 1989. 14. Liu DY and Baker HWG. Hypoosmotic sperm swelling test as a sperm function test. Fertility and Sterility 51:365-366, 1989. 15. Liu DY, Clarke GN, Lopata A, Johnston WIH and Baker HWG. A sperm-zona pellucida binding test and in vitro fertilization. Fertility and Sterility 52: 281-287,1989. 16. Baker HWG and Liu DY. New tests of human sperm function and in vitro fertilization. Reproduction, Fertility and Development 1:179-182, 1989. 17. Liu DY and Baker HWG. Inducing the human acrosome reaction with a calcium ionophore A23187 decreases sperm-zona pellucida binding with oocytes that failed to fertilize in vitro. Journal of Reproduction and Fertility 89:127-134, 1990. 18. Liu DY and Baker HWG. Relationships between human sperm acrosin, acrosomes, morphology and in vitro fertilization. Human Reproduction. 5:298-303, 1990. 19. O'Bryan MK, Baker HWG, Saunders JR, Kirszbaum L, Walker ID, Hudson, P, Liu DY, d'Apice AJF and Murphy BF. Human seminal clusterin (SP40,40). Isolation and characterization. Journal of Clinical Investigation. 85:1477-1486, 1990. 20. Liu DY, Lopata A and Baker HWG. Use of oocytes that failed to be fertilized in vitro to study sperm-oocyte interaction: comparison of sperm-oolemma and sperm-zona pellucida binding, and relationship with results of IVF. Reproduction, Fertility and Development. 2:641-650, 1990. 21. Liu DY, Clarke GN and Baker HWG. Inhibition of human sperm-zona pellucida and sperm-oolemma binding by antisperm antibodies. Fertility and Sterility. 55:440-442, 1991. 22. Liu DY, Clarke GN and Baker HWG. Relationship between sperm motility assessed with the Hamilton- Thorn Motility Analyzer and fertilization rates in vitro. Journal of Andrology, 12:231-239, 1991. 23. Liu DY, Lopata A, Pantke P and Baker HWG. Horse and marmoset sperm bind to the zona pellucida of salt stored human oocytes. Fertility and Sterility, 56:764-767, 1991. 24. Liu DY and Baker HWG. Morphology of spermatozoa bound to the zona pellucida of human oocytes that failed to fertilize in vitro. Journal of Reproduction and Fertility, 94:71-84, 1992. 25. Ng FLH, Liu DY and Baker HWG. Comparison of Percoll, mini-percoll and swim-up methods for sperm - 2 - preparation from abnormal semen samples. Human Reproduction, 7:261-266, 1992. 26. Liu DY and Baker HWG. Tests of human sperm function and fertilization in vitro. Fertility and Sterility, 58:465-483, 1992. 27. Liu DY and Baker HWG. Sperm nuclear chromatin normality: relationship with sperm morphology, sperm- zona pellucida binding and fertilization rates in vitro. Fertility and Sterility 58:1178-1184, 1992. 28. Liu DY and Baker HWG. Inhibition of acrosin activity with a Trypsin inhibitor blocks human sperm penetration of the zona pellucida. Biology of Reproduction 48:340-348, 1993. 29. Baker HWG and Liu DY, Bourne H and Lopata A. Diagnosis of sperm defects in selecting patients for assisted fertilization. Human Reproduction, 8:1779-1780, 1993. 30. Liu DY and Baker HWG. Tests of sperm function. Fertility and Sterility, 59:698-699, 1993. 31. Liu DY and Baker HWG. A new test for the assessment of sperm-zona pellucida penetration: relationship with results of other sperm tests and fertilization in vitro. Human Reproduction, 9:489-496, 1994. 32. Liu DY and Baker HWG. Acrosome status and morphology of human sperm bound to the zona pellucida and oolemma determined using oocytes that failed to fertilize in vitro. Human Reproduction, 9:673-679,1994. 33. Liu DY and Baker HWG. Disorderd acrosome reaction of sperm bound to the zona pellucida (ZP). a newly discovered sperm defect causing infertility with reduced sperm-ZP penetration and reduced fertilization in vitro. Human Reproduction, 9:1694-1994, 1994. 34. O'Bryan MK, Murphy BF, Liu DY, Clarke GN and Baker HWG. The use of anticlusterin monoclonal antibodies for the combined assessment of human sperm morphology and acrosome integrity. Human Reproduction, 9:1490-1496, 1994. 35. Clarke,GN, Liu DY and Baker HWG. Immunoinfertility: a case study with implications for immunocontraception. Archives of Andrology, 35:21-27, 1995. 36. Liu DY, Robyn Johnston R and Baker HWG. Ability of sperm to bind to the zona pellucida during oligozoospermia induced with testosterone during a male contraceptive trial. International Journal of Andrology 18 (Suppl 1):39-44, 1995 1995. 37. Johnston RC, Clarke GN, Liu DY, and Baker HWG. Assessment of the Sperm Quality Analyser (SQA). Fertility and Sterility, 63:1071-1076, 1995. 38. Liu DY, Bourne H and Baker HWG. Fertilization and pregnancy with acrosome intact sperm by intracytoplasmic sperm injection in patients with disordered zona pellucida induced acrosome reaction. Fertility and Sterility, 64:116-121, 1995. 39. Bourne H, Liu DY, Clarke GN and Baker HWG. Normal fertilization and embryo development with an round headed acrosomeless sperm. Fertility and Sterility, 63:1329-1332, 1995. 40. Pantke P, Hyland J, Galloway DB, Liu DY and Baker HWG. Development of a zona pellucida sperm binding assay for the assessment of stallion fertility. Biology of Reproduction, Monograph 1 (Equine Reproduction VI):681-687, 1995. 41. Bourne H, Richings N, Liu DY, Clarke GN, Harari O and Baker HGW. Sperm preparation for intracytoplasmic injection: methods and relationship to fertilization results. Fertility, Reproduction and Development, 7:177-183, 1995. - 3 - 42. Liu DY and Baker HWG. A simple method for assessment of the human acrosome reaction of spermatozoa bound to the zona pellucida: lack of relatioship with ionophore A23187-induced acrosome reaction. Human Reproduction, 11:551-557, 1996. 43. Liu DY and Baker HWG. Relationship between the zona pellucida (ZP) and ionophore A23187 induced acrosome reaction and the ability of sperm to penetrate the ZP in men with normal sperm-ZP binding. Fertility and Sterility, 66:312-315, 1996. 44. Garrett C, Liu DY and Baker HWG. Selectivity of the human sperm-zona pellucida bining process to sperm head morphometry. Fertility and Sterility, 67, 362-371, 1997. 45. Liu DY, Bourne H and Baker HWG. High fertilization and pregnancy rates after intracytoplasmic sperm injection in patients with disordered zona pellucida-induced acrosome reaction. Fertility and Sterility. 67, 955-958, 1997. 46. Liu DY, Baker HWG, Pears MJ and d Apice AJF. Normal sperm-zona pellucida interaction and fertilization in vitro in a-1,3-galactosyltransferase gene knockout mice. Molecular Human Reproduction. 3, 1015-1016, 1997. 47. Liu DY and Baker HWG. Protein Kinase C plays an important role in human zona pellucida-induced acrosome reaction. Molecular Human Reproduction, 3, 1037-1043, 1997. 48. Liu DY and Baker HWG. Calcium ionophore-induced acrosome reaction correlates with fertilization rates in vitro in patients with teratozoospermic semen. Human Reproduction, 13:905-910, 1998. 49. Liu DY, Martic M, Clarke GN, Dunlop ME and Baker HWG. An important role of actin polymerization in the human zona pellucida-induced acrosome reaction. Molecular Human Reproduction, 5: 941-949, 1999. 50. Liu DY and Baker HWG. Defective sperm-zona pellucida interaction: a major cause of failure of fertilization in clinical in-vitro fertilization. Human Reproduction, 15: 702-708, 2000. 51. Baker HWG, Liu DY, C Garrett and M Martic The human acrosome reaction. Asian Journal of Andrology, 2: 172-178, 2000. 52. Liu DY, Clarke GN, Martic M, Garrett C and Baker HWG. Frequency of disordered zona pellucida (ZP)- induced acrosome reaction in infertile men with normal semen analysis and normal sperm-ZP binding. Human Reproduction, 6: 1185-1190, 2001. 53. Liu DY, Martic M, Clarke, GN, Grkovic I, Garrett C, Dunlop ME and Baker HWG. An anti-actin monoclonal antibody inhibits the zona pellucida induced acrosome reaction and hyperactivated motility of human sperm. Molecular Human Reproduction, 8, 37-47, 2002. 54. Liu DY, Martic M, Garrett C, Dunlop ME and Baker HWG. Phorbol myristate acetate induces ruffling of the acrosome of human spermatozoa. Fertility and Sterility, 78: 128-136, 2002. 55. Liu DY and Baker HWG. Disordered zona pellucida induced acrosome reaction and failure of in vitro fertilization in patients with unexplained infertility. Fertility and Sterility, 79: 74-80, 2003. 56. Liu, DY and Baker HWG. Frequency of defective sperm-ZP interaction in severely teratospermic infertile men. Human Reproduction, 18: 802-807, 2003. 57. Liu DY, Stewart T and Baker HWG. Normal range and variation of the zona pellucida-induced acrosome reaction in fertile men. Fertility and Sterility, 80: 384-389, 2003. - 4 - 58. Garrett C, Liu DY, Clarke GN, Rushford, DD and Baker, HWG. Automated semen analysis variables : zona pellucida preferred sperm morphometry and straight line velocity, are related to pregnancy rate in subfertile couples. Human Reproduction, 18: 1643-1649, 2003. 59. Liu DY, Garrett C and Baker HWG. Low proportions of spermatozoa can bind to the zona pellucida of human oocytes. Human Reproduction, 18: 2382-2389, 2003. 60. Clarke GN, Liu DY and Baker HWG. Improved sperm cryopreservation after very rapid cooling above the freezing point. Reproduction, Fertility and Development, 15: 377-381, 2003. 61. Liu, DY and Baker HWG. High frequency of defective sperm-ZP interaction in oligozoospermic infertile men. Human Reproduction, 19:228-233, 2004. 62. Martic M, Moses E, Adams T, Liu DY, Gook D, Garrett C, Dunlop ME, Baker HWG Recombinant human zona pellucida proteins ZP1, ZP2 and ZP3 co-expressed in a human cell line. Asian Journal of Andrology. 6: 3-13, 2004. 63. Apedaile AE, Garrett C, Liu DY Clarke GN, Johnston SA and Baker HWG. Flow cytometry and microscopic acridine orange test: relationship with standard semen analysis. Reproductive BioMedicine Online. 8 (4):398-407, 2004. 64. Liu DY, Zhu WJ and Baker HWG. Importance role of assessment of sperm function in clinical management of infertility in IVF/ICSI. Reproduction & Contraception (Chinese) 24 (4): 193-199, 2004. 65. Liu DY, Garrett C and Baker HWG. Clinical application of sperm-oocyte interaction tests in in vitro fertilization-embryo transfer and intracytoplasmic sperm injection programs (Modern Trends). Fertility and Sterility 82 (5):1251-1263, 2004. 66. Liu DY, Clarke GN and Baker HWG. Exposure of actin on the surface of the human sperm head during in vitro culture relates to sperm morphology, capacitation and zona binding. Human Reproduction 20 (4): 999-1005, 2005. 67. Garrett C, Liu DY, McLachlan RI and Baker HWG. Time course of changes in sperm morphometry and semen variables during testosterone induced suppression of human spermatogenesis. Human Reproduction 20 (11), 3091-3100, 2005. 68. Liu DY, Clarke GN and Baker HWG. Hyper-osmotic condition enhances protein tyrosine phosphorylation and zona pellucida binding capacity of human spermatozoa. Human Reproduction 21(3), 745-752, 2006. 69. Liu DY, Clarke GN and Baker HWG. Tyrosine phosphorylation of capacitated human sperm tail detected by immunofluorescence correlates with sperm-zona pellucida (ZP) binding and not the ZP-induced acrosome reaction. Human Reproduction 21 (3), 1002-1008, 2006. 70. Liu DY, Garrett C and Baker HWG. Acrosome reacted sperm in medium do not bind to the zona pellucida of human oocytes. International Journal of Andrology, 29,475-481, 2006. 71. Ye H, Huang GN, Gao Y and Liu DY. Relationship between sperm-hyaluronan binding assay and fertilization rate in conventional in vitro fertilization. Human Reproduction, 21(6), 1545-1550, 2006. 72. Clarke GN, Liu DY and Baker HWG. Recovery of human sperm motility and ability to interact with the human zona pellucida after more than 28 years of storage in liquid nitrogen. Fertility and Sterility, 86 (3), 721-722, 2006. - 5 - 73. Liu DY and Baker HWG. Assessment of human sperm function and clinical management of male infertility. National Journal of Andrology (Natl J Androl) 13 (2), 99-109, 2007. 74. Liu DY and Baker HWG. Human sperm bound to the zona pellucida have normal nuclear chromatin assessed by Acridine Orange fluorescence. Human Reproduction 22 (6): 1597-1602, 2007. 75. Liu DY, Liu ML, Garrett C and Baker HWG. Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen. Human Reproduction 22 (7): 1878-1884, 2007. 76. Liu DY, Liu ML, Clarke GN and Baker HWG. Hyperactivation of capacitated human sperm correlates with the human zona pellucida-induced acrosome reaction of zona pellucida-bound sperm. Human Reproduction 22 (10): 2632-2638, 2007. 77. Garrett C, Liu DY and Baker HWG. Comparison of human sperm morphometry assessment models based on zona pellucida selectivity. Society of Reproduction and Fertility Supplement 65, 357-361, 2007. 78. Liu DY, Liu ML and Baker HWG. Enhancement of sperm-zona pellucida (ZP) binding capacity by activation of protein kinase A and C pathways in certain infertile men with defective sperm-ZP binding. Human Reproduction 24 (1): 20-27, 2009. 79. Stewart TM, Liu DY, Garrett C, Jørgensen N, Brown EH and Baker HWG. Associations between andrological measures, hormones and semen quality in fertile Australian men: inverse relationship between obesity and sperm output. Human Reproduction 24 (7): 1561-1568, 2009. 80. Liu DY, Sie BS, Liu ML, Agresta F and Baker HWG. Relationship between seminal plasma zinc concentration and sperm-zona pellucida (ZP)-binding and the ZP-induced acrosome reaction in subfertile men. Asian Journal of Andrology 11: 499-507, 2009. 81. Stewart TM, Liu DY, Garrett C, Brown EH and Baker HWG. Recruitment bias in retrospective time to pregnancy studies of factors affecting human fertility Human Reproduction, 24 (10):2401-2408, 2009. 82. Liu DY and Baker HWG. Prediction of failure of fertilization in IVF. Journal of Reproductive Medicine (Chinese) 18 (3): 202-205, 2009 83. Black M, Liu DY, Bourne H and Baker HWG. Comparison of outcomes of conventional intracytoplasmic sperm injection (ICSI) and ICSI using sperm bound to the zona pellucida of immature oocytes. Fertility and Sterility 93: 672-674, 2010. 84. Veaute C, Liu DY, Furlong LI, Carlos BJ, Baker HWG and Vazquez-Levin MH. Anti-human proacrosin antibody inhibits the zona pellucida (ZP)-induced acrosome reaction of ZP-bound spermatozoa. Fertility and Sterility 93: 2456-2459, 2010. 85. Liu F, Qiu Y, Zou Y, Deng ZH, Yang H, Liu DY. Use of zona pellucida-bound sperm for intracytoplasmic sperm injection produces higher embryo quality and implantation than conventional intracytoplasmic sperm injection. Fertility and Sterility 95: 815-818, 2011. 86. Liu DY. Could using the zona pellucida bound sperm for intracytoplasmic sperm injection (ICSI) enhance the outcome of ICSI? Asian Journal of Andrology 13: 197-198, 2011. 87. Wang W, Liang GT, Peng YY, Liu DY, Zhou XM. [Effects of a microfluidic sperm sorter on sperm routine parameters and DNA integrity]. Zhonghua Nan Ke Xue. 2011 Apr;17(4):301-4. Chinese. - 6 - 88. Liu DY, Wang MW, Wang J, Xia WM, Zhang CY, Zhao JP, Zhou WL. [Retropubic radical prostatectomy: 10 years' experience with 100 cases]. Zhonghua Nan Ke Xue. 2011 Jun;17(6):523-6. Chinese.

Publications (Book Chapters) 1. Baker HWG, Ng FLH and Liu DY. Preparation and analysis of semen for IVF/GIFT. In Handbook of In Vitro Fertilization. (1st Edition) Trounson A. O. and Gardner D.K., (eds), CRC press, Boca Raton, pp34-56, 1993. 2. Liu DY and Baker HWG. Tests for human sperm-zona pellucida binding and penetration. In Male Factor in Human Infertility, Tesarik, J. (ed), Ares-Serono Symposia Publications, Frontiers in Endocrinology, Volume 8. Rome, Italy, pp169-185, 1994. 3. Bourne H, Richings N, Liu DY, Clarke GN, Harari O and Baker HGW. Sperm preparation for intracytoplasmic injection: methods and relationship to fertilization results. In Intracytoplasmic Sperm Injection-The Revolution in Male Infertility,. Flaherty S. P. and Matthews C.D. (eds), CSIRO Australia, pp43-49. 1995. 4. Baker HWG and Liu DY. Assessment of nuclear maturity. In Human Spermatozoa in Assisted Reproduction (2nd Edition), Acosta, A.A. and Kruger, T.F. (eds), The Parthenon Publishing Group, New York, pp93-203, 1996. 5. Baker HWG, Liu DY and Bourne H. Assessment of the male and preparation of sperm for ARTs. In Handbook of In Vitro Fertilization. (2nd Edition), Trounson A.O. and Gardner D.K., (eds), CRC press, Boca Raton, pp99-126, 1999. 6. Baker HWG, Clarke GN, Liu DY, Stewart TM and Garrett C. Semen analysis and sperm function testing. In Male Reproductive Dysfunction: Pathophysiology and Treatment, Kandeel F.R. and Swerdloff R.S. (eds), Informa Healthcare USA, Inc New York 271-283, 2007. 7. Liu DY, Bourne H, Garrett Claire, Barak S, Baker HWG Sperm Assessment: Novel Approaches and Their Indicative Value, In:, Nagy ZP, Varghese A and Agarwal A (eds.) Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices, Humana Press (In Press 2010)

Invited Lectures

1. Liu DY, Human sperm functions and clinical IVF, Department of Obstetrics and Gyanecology, University of Adelaide, South Australia, 16 April 1991.

2. Liu DY, Human sperm function. Satellite Symposium on Andrology, Beijing, China, 8-10 May 1993.

3. Liu DY, Semen analysis and new sperm function tests. WHO sponsored Andrology-Vas Occlusion Techniques Workshop, Hanoi, Viet Nam, 23 November to 2 December 1993.

4. Liu DY, Tests of human sperm-zona pellucida binding and penetration. International Symposium on Male Factor in Human Infertility, Paris, France, 21-22 April 1994.

- 7 - 5. Liu DY, Defects of human sperm-oocyte interaction. Postgraduate Course of the VIth International Congress of Andrology, Salzburg, Austria, 29-29 May 1997.

6. Liu DY, The human acrosome and sperm-oocyte interaction reaction. International Symposium for Young Chinese Reproductive Biologists, Beijing, China,14-18 October 1997.

7. Liu DY. Sperm-oocyte interaction tests. In Clinical Application of Advances in Andrology. Fertility Society of Australia (FSA) Satellite Symposium (Honour of Professor Colin Matthews). Hobart, Australia, 28th October 1998.

8. Liu DY. Clinical application of sperm function tests in IVF/ICSI. The 1st Chongqing ICSI Workshop, Chongqing, China, 19-22 November 1999.

9. Liu DY. Defective sperm function and male infertility. Symposium on Establishment of Research and Business Cooperation between Chinese Experts and Overseas Chinese Scholars. Guiling, Guangxi, China, 17 to 21 December 2001.

10. Liu DY. Implication of sperm function assessment in clinical IVF/ICSI. The 4th Chongqing ART workshop, Chongqing, China, 2-4 September 2002.

11. Liu DY. Evaluation and assessment of semen for IVF/ICSI. The First Asia-Pacific Forum on Andrology, Shanghai, China, 17-21 October 2002.

12. Liu DY. Sperm-zona pellucida interaction: an important sperm function test for diagnosis and management of unexplained male infertility in clinical IVF/ICSI. The 5th ART workshop, Chongqing, China, 16-18 December 2003.

13. Liu DY. Assessment of sperm function for diagnosis and management of male infertility in clinical IVF/ICSI. The 1st International forum on Reproductive Medicine and the 1st Asia Community Genetics Workshop, Shenzhen, China, 10-18, April 2004.

14. Liu DY. Importance of assessment of human sperm function in clinical IVF/ICSI. The 2nd International Congress of Reproductive Medicine, the 1st Affiliated Hospital of Lanzhou Medical University, Lanzhou, China, 8-10 September 2004.

15. Liu DY. Laboratory aspects and clinical application of new sperm function tests. Bangkok Andrology Workshop 2004, Bangkok, Thailand, 19 October 2004.

16. Liu DY. Basic facilities of Andrology Lab and useful sperm function tests in clinical ART. The 6 th Chongqing ART workshop, Chongqing, China, 22-23, December 2004.

17. Liu DY. Workshop on semen analysis and sperm function tests. Chongqing, China, 27-28, May 2005.

18. Liu DY. Application of sperm function tests in clinical ART. International Conference on Male Reproduction and Infertility, Indian Institute of Science, Bangalore, India, 16-18 September 2005.

19. Liu DY. Assessment of human sperm chromatin structure damage and outcomes of ART. 8th Chongqing ART Workshop, Chongqing, China, 20-22, April, 2006. - 8 - 20. Liu DY. Importance of assessment of human sperm function in clinical ART. The 2nd Asia Pacific Forum on Andrology, Shanghai, China, 26-30, October 2006.

21. Liu DY. Application of sperm function tests in clinical ART. The 9th Chongqing ART Workshop, Chongqing, China, 19-21, May, 2007.

22. Liu DY. Morphology and nuclear chromatin of sperm bound to the zona pellucida. The 10th Chongqing ART Workshop, Chongqing, China, 28-31, August, 2008.

23. Liu DY. Importance of assessment of sperm function in clinical IVF/ICSI . Symposium on sperm function and fertility. The 2nd Nanning People’s Hospital, Nanning, Guangxi, China, 6-7 December, 2008.

24. Liu DY. Prediction of failure of fertilization in clinical IVF. Merck-Serono Symposium on Fertilization and its Failure. Sanya, Hainan, China 21-22 March 2009.

25. Liu DY. Evaluating sperm function. The 3nd Asia Pacific Forum on Andrology, Nanjing, China, 10-13, October 2009.

26. Liu DY. Sperm DNA damage and ART outcomes. Symposium on sperm function and fertility. The 11th Chongqing ART Workshop, Chongqing, China, 13-15, October, 2009.

Abstract or conference presentations Dr Liu had 102 abstract/papers (as senior or co-author) presented in national and international scientific meetings.