Supplementary Table 6. Homologues of BrANSs and their predicted functional partners predicted by using STRING 9.1a Query Bit STRING protein Identity sequence score LDOX (leucoanthocyanidin dioxygenase); encodes leucoanthocyanidin dioxygenase, which BrANS1 LDOX is involved in proanthocyanin biosynthesis. 92 % 669 and 3 Oxidation of leucoanthocyanidins into anthocyanidins 2OG-Fe(II) oxygenase family protein; BrANS2 AT2G38240 oxidoreductase, involved in: response to salt 84 % 622 and 4 stress MYB2 (myb domain protein 2); DNA binding / calmodulin binding / transcription activator/ transcription factor; Encodes a MYB transcription BrMYB2-2 MYB2 factor that possesses an R2R3 MYB DNA 86 % 495 binding domain and is known to regulate the expression of salt- and dehydration-responsive genes. TT8 (transparent testa 8); DNA binding / transcription factor; TT8 is a regulation factor that acts in a concerted action with TT1, PAP1 and TTG1 on the regulation of flavonoid pathways, namely proanthocyanidin and anthocyanin biosynthesis. Affects BrTT8 TT8 dihydroflavonol 4-reductase gene expression. It is 73 % 740 thought that a ternary complex composed of TT2, TT8 and TTG1 is necessary for correct expression of BAN in seed endothelium. Also important for important for marginal trichome development. ; Transcription activator, when associated with MYB75/PAP1 or MYB90/PAP2. a STRING 9.1 (http://string-db.org/newstring_cgi/show_input_page.pl? UserId=0FXEANviwLB3&sessionId=Inku_BVeQnW5&input_page_type=multiple_identifiers)

Predicted Functional Partners: Score TT2 TT2 (transparent testa 2); DNA binding / transcription factor; (258 aa) 0.999 MYB75 PAP1 (production of anthocyanin pigment 1); DNA binding / 0.999 transcription factor; (248 aa) TTG1 TTG1 (transparent testa glabra 1); DNA binding / nucleotide binding / 0.997 protein binding; (341 aa) DFR DFR (dihydroflavonol 4-reductase); dihydrokaempferol 4-reductase; 0.997 dihydroflavonol reductase. (382 aa) BAN BAN (banyuls); oxidoreductase; Negative regulator of flavonoid 0.991 biosynthesis, (340 aa) TT7 TT7 (transparent testa 7); flavonoid 3'-monooxygenase/ oxygen binding; 0.989 (513 aa) UF3GT UF3GT (udp-glucose-flavonoid 3-O-glucosyltransferase); transferase, 0.978 transferring glycosyl group (468 aa) ATGSTF12 ATGSTF12 (arabidopsis thaliana glutathione S-transferase PHI 12); 0.977 glutathione transferase; (214 aa) AT4G1409 UDP-glucoronosyl/UDP-glucosyl transferase family protein; The 0.971 0 At4g14090 encodes a anthocyanidin (456 aa) MYC2 MYC2; DNA binding / transcription activator/ transcription factor; 0.970 Transcriptional activator. (623 aa)