Proteomics Course Syllabus

Course Title: Current Proteomics (PHRM 555)

Credit: 3

Instructors: Masaru Miyagi (Course Director), Elizabeth Yohannes, Janna Kiselar, Rob Ewing, Jean-Eudes Dazard, Mark Chance, Sayan Gupta

Class time and location: TBA

Course Description: This course is designed for graduate students across the university who wish to acquire a better understanding of fundamental concepts of proteomics and hands-on experience with techniques used in current proteomics. Lectures will cover protein/peptide separation techniques, protein mass spectrometry, bioinformatics tools, biostatistics and biological applications which include quantitative proteomics, protein modification proteomics, interaction proteomics, protein biomarker development, structural mass spectrometry. Laboratory portion will involve practice on the separation of proteins by two-dimensional gel electrophoresis, molecular weight measurement of proteins by mass spectrometry, peptide structural characterization by tandem mass spectrometry and protein identification using computational tools. The instructors’ research topics will also be discussed.

Exam: The exam will be to write a ~5 pages research proposal applying proteomic approaches.

Grading Policy: - Journal presentation will count for 20% of the final grade - Lab reports: 20% - Proposal presentation: 20% - Final proposal: 40%

Reading Material and Lecture Notes: PowerPoint presentations from each class will be posted after each lecture. All of the reading material for the course will also be posted on the course webpage. No textbook is required. We will let everyone know what reading material is recommended for each upcoming class.