OIE Reference Laboratory Reports s5

OIE Reference Laboratory Reports Activities in 2011

Name of disease (or topic) for Newcastle disease which you are a designated OIE Reference Laboratory:

Address of laboratory: Newcastle disease laboratory Animal Plant and Fisheries Quarantine & Inspection Agency (QIA), Ministry of Food, Agriculture, Forestry and Fisheries (MIFAFF) 175 Anyang-ro, Manan-gu, Anyang, Gyeonggi, 430-757, REPUBLIC OF KOREA

Tel.: (82) 31-467-1821

Fax: (82) 31-467-1814

e-mail address: [email protected]

website: http://www.qia.go.kr/english/html/indexqiaEngNoticeWebAction.do

Name (including Title and Dr Yong-Ho Park Position) of Head of DVM, MSc, PhD Laboratory (Responsible QIA Commissioner Official):

Name(including Title and Dr Kang-Seuk Choi Position) of OIE Reference DVM, MSc, PhD Expert: Chief of Newcastle disease laboratory

Name (including Title and Position) of writer of this report (if different from above):

Annual reports of OIE Reference Centres, 2011 1 Newcastle disease

Part I: Summary of general activities related to the disease

1. Test(s) in use/or available for the specified disease/topic at your laboratory

Test For Specificity Total

Egg inoculation Virus isolation Group 1881

RT-PCR Antigen detection Group, pathotype 181

Nucleotide sequencing Molecular test Pathotype, genotype 47

HI serology Antibody detection Serotype 2004(total) APMV-1(NDV) 1857 APMV-2 21 APMV-3 21 APMV-4 21 APMV-6 21 APMV-7 21 APMV-8 21 APMV-9 21

2. Production and distribution of diagnostic reagents

 A total of 8,300 tests of a RT-PCR detection kit for NDV were purchased from a commercial company and distributed to 20 local diagnostic laboratories for diagnostic purpose this year.  No requests for reagents were received this year from other OIE member Counties.

Part II: Activities specifically related to the mandate of OIE Reference Laboratories

3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines

a) Establishment and maintenance of a network with other OIE Reference Laboratories designated for the same pathogen or disease and organisation of regular inter-laboratory proficiency testing to ensure comparability of results

Dr KS Choi took a part in validation and certification of diagnostic assays for Newcastle disease as a reviewer, upon request from the biological Standards commission of OIE.

b) Organisation of inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results

4. Preparation and supply of international reference standards for diagnostic tests or vaccines

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5. Research and development of new procedures for diagnosis and control

A research project on the development of a chimeric type of Newcastle disease vaccine that can differentiate vaccination from infection has been performed in combination with the development of an ELISA system featuring the DIVA (differentiation of infected from vaccinated animals) strategy. The vaccine can be applied for the control of Newcastle disease in the form of live or inactivated vaccine in poultry.

A research project on the development of a live Newcastle disease virus vaccine strain for the control of Newcastle disease in poultry is in progress. Efficacy tests including immune response, experimental challenge and field trial of the vaccine candidate are under investigation.

A research project on the development of new HI diagnostic antigen is also in progress. The antigen was produced in the form of recombinant HN protein of NDV in order to replace the whole virus based HI antigen. The specificity and sensitivity of the new antigen are under study in accordance with the OIE Validation Template.

5. Collection, analysis and dissemination of epizootiological data relevant to international disease control

A nation-wide surveillance of wild birds in Korea especially belonging to the family Anatidae for the presence of avian paramyxoviruses has initiated this year. The surveillance data might extend the knowledge of the epidemiology of NDV in wild birds in connection with that in poultry.

OIE reference laboratory for Newcastle disease in Korea has initiated two research projects on the epidemiology of Newcastle disease virus in wild birds and poultry in Asia from year 2011 to 2013, by performing the international collaborative studies with Vietam (National center for Veterinary Diagnosis) and the kingdom of Cambodia (National Veterinary Research Institute).

7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned

OIE reference laboratory for Newcastle disease in Korea maintains a laboratory with a bio-safety level II plus facility suitable for handling virulent NDVs under the regulation of Animal Plant Fisheries Quarantine and Inspection Agency (QIA).

8. Provision of consultant expertise to OIE or to OIE Member Countries

Dr KS Choi reviewed the draft of revised version of Newcastle disease section for the OIE Manual of Diagnostic tests and vaccines for terrestrial animals.

Dr KS Choi participated in reviewing documents for OIE procedures for validation and certification of diagnostic assays on Newcastle disease, as requested by the biological Standards commission of OIE.

Dr KS Choi offered technical advice on the preventive measures against epizootic of Newcastle disease in poultry to Department of Veterinary Services of Malaysia (18 March 2011) and Malaysian Association of Food Animal Veterinarians (17 March 2011).

9. Provision of scientific and technical training to personnel from other OIE Member Countries

Training for the molecular and classical techniques of NDV diagnosis was provided to the scientist (Dr Le Van Phan) from Vietnam (September 2010- August 2011).

10. Provision of diagnostic testing facilities to other OIE Member Countries

Annual reports of OIE Reference Centres, 2011 3 Newcastle disease

11. Organisation of international scientific meetings on behalf of OIE or other international bodies

12. Participation in international scientific collaborative studies

OIE reference laboratory for Newcastle disease in Korea has initiated two international research this year in order to extend the understanding of epidemiological situation of NDV in Asia; comparative studies for avian influenza virus (AIV) and Newcastle disease virus (NDV) between Korea and Vietnam (National center for Veterinary Diagnosis) for two years from June 2011 to May 2013 and Study on the molecular epidemiology of Newcastle disease and avian influenza viruses in Cambodia(National Veterinary Research Institute) from October 2011 to September 2013.

13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)

13.1. Presentations at international conferences and meetings

 Lee EK, Choi KS, Jeon WJ, Park MJ, Yoo YN, Kwon JH. Molecular characterization of Newcastle disease viruses isolated chicken farms in Malaysia. International Union of Micrological Societies 2011 congress. 11- 16 September 2011, Sapporo Convention Center, Japan.  Choi KS. Newcastle disease in Asia. An international conference, organized by the Malaysian Association of Food Animal Veterinarians, and supponsored by world poultry society association (WPSA) Malaysia and Nutri Pharmax SDN. 17 March 2011. Holliday Villa Subang Hotel, Kuala pur, Malaysia.

13.2. Scientific publications in peer-reviewed journals

 Choi KS, Lee EK, Jeon WJ, batchuulon D, Sodnomdarjaa R, Park MJ, Yoo YN, Kwon JH. Molecular biological characterization of the first Newcastle disease virus isolated in Mongolia. Korean Journal of Poultry Science. 38(2):89-96, 2011.  Choi KS, Lee EK, Jeon WJ, Kwon JH, Lee J-H, Sung HW. Surveillance of Newcastle disease virus at slaughterhouses and slaughtered chickens in Korea. Korean Journal of Poultry Science. 38(2):97-104, 2011.  Choi KS, Jeon WJ, Lee EK, Kwon JH, Sung HW. Monitoring of major avian viral pathogens’ contamination in new and reused broiler litters. Korean Journal of Poultry Science. 38(3):181-189, 2011.

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