The 72 Conifer Species Screened in This Study

Table S1 The 72 conifer species screened in this study. Species OneKP Transcriptome Identifiers Abies lasiocarpa VSRH Acmopyle pancheri HILW Agathis robusta MIXZ Amentotaxus argotaenia IAJW Araucaria rulei XTZO Arucaria sp. ACWS Athrotaxis cupressoides XIRK Austrocedrus chilensis YYPE Austrotaxus spicata BTTS Callitris gracilis IFLI Callitris macleayana RMMV Calocedrus decurrens FRPM Cathaya agryrophylla NPRL Cedrus libani GGEA Cephalotaxus harringtonia NVGZ Chamaecyparis lawsoniana AIGO Cryptomeria japonica GMHZ Cunninghamia lanceolata ZQVF Cupressus dupreziana QNGJ Dacrycarpus compactus FMWZ Dacrydium balansae IZGN Diselma archeri GKCZ Falcatifolium taxoides PLYX Fokienia hodginsii UEVI Glyptostrobus pensilis OXGJ Halocarpus bidwillii OWFC Juniperus scopulorum XMGP Keteleeria evelyniana JUWL Lagarostrobos franklinii ZQWM Larix speciosa WVWN Manoao colensoi CDFR Metasequoia glyptostroboides NRXL Microbiota decussata XQSG Microcachrys tetragona MHGD Microstrobos fitzgeraldii BBDD Nageia nagi UUJS Neocallitropsis pancheri JDQB Nothotsuga longibracteata AREG Papuacedrus papuana OVIJ Parasitaxus usta JZVE Phyllocladus hypohyllus JRNA Picea engelmanii AWQB Pilgerodendron uviferum ETCJ Pinus jeffreyi MFTM Pinus parviflora IIOL Pinus ponderosa JBND Pinus radiata DZQM Platycladus orientalis BUWV Podocarpus coriaceus SCEB Podocarpus rubens XLGK Prumnopitys andina EGLZ Pseudolarix amabilis AQFM Pseudotaxus chienii YLPM Pseudotsuga menziesii IOVS Retrophyllum minus VGSX Saxegothaea conspicua QCGM Sciadopitys verticillata YFZK Sequoia sempervirens HBGV Sequoiadendron giganteum QFAE Sundacarpus amarus KLGF Taiwania cryptomerioides QSNJ Taxodium distichum FHST Taxus baccata WWSS Taxus cuspidata ZYAX Tetraclinis sp. CGDN Thuja plicata VFYZ Thujopsis dolabrata NKIN Torreya nucifera HQOM Torreya taxifolia EFMS Tsuga heterophylla GAMH Widdringtonia cedarbergensis AUDE Wollemia nobilis RSCE Table S2 CYP79A genes and gene fragments identified in this study. The numbers in brackets given in the ‘comments’ column indicate the length of the encoded proteins, and the four-letter codes in the ‘Species’ column are OneKP transcriptome identifiers. Species Family Designation Comments Phyllocladus hypophyllus (JRNA) Podocarpaceae fragment (380 AA) Phyllocladus hypophyllus (JRNA) Podocarpaceae fragment (446 AA) Dacrydium balansae (IZGN) Podocarpaceae CYP79A120 full-length (537 AA) Falcatifolium taxoides (PLYX) Podocarpaceae CYP79A121 full-length (540 AA) Dacrycarpus compactus (FMWZ) Podocarpaceae CYP79A119 full-length (533 AA) Podocarpus coriacens (SCEB) Podocarpaceae fragment (389 AA) Podocarpus rubens (XLGK) Podocarpaceae fragment (338 AA) Cephalotaxus harringtonia (NVGZ) Taxaceae fragment (487 AA) Amentotaxus argotaenia (IAJW) Taxaceae CYP79A122 full-length (537 AA) Torreya taxifolia (EFMS) Taxaceae CYP79A123 full-length (519 AA) Taxus baccata (WWSS) Taxaceae CYP79A118 full-length (582 AA) Metasequoia glyptostroboides (NRXL) Cupressaceae CYP79A117 full-length (557 AA) Table S3 Oligonucleotides used for isolation and qRT-PCR analysis of conifer CYP79 genes. Name Sequence Usage WWSS9882-fwd ATGGTGTGGGGATTTGTTTTC ORF cloning WWSS9882-rev CTATGGATAGAGATGGTTGGGA ORF cloning

WWSS-NotI_fwd_WT CTTTGCGGCCGCAATGGTGTGGGGATTTGTTTTC expression M1 WWSS-NotI_fwd_M38 GTTAGCGGCCGCAATGCGCCATCATCTTCATATTG expression M38 WWSS-SacI_rev GGGTGAGCTCCTATGGATAGAGATGGTTGGG expression

TbCyp79_fwd3 TGTCAATGGAGGACACCACAG qRT-PCR TbCyp79_rev3 GAGGATCCTCCCACACACATG qRT-PCR TbGAPDH_fwd1 CGGAGACAGTCGATCAAGC qRT-PCR TbGAPDH_rev1 CCCATCCTCAACCCAATAA qRT-PCR TbActin_fwd1 AAGAGAAGCTTGCTTATGTAGC qRT-PCR TbActin_rev1 TCTGATATCCACATCACACTTC qRT-PCR Tb18S-RNA_fwd1 GTGCACAAAATCCCGACTCT qRT-PCR Tb18S-RNA_rev1 GCGATCCGTCGAGTTATCAT qRT-PCR