De Novo Auxin Biosynthesis Is Necessary for Flower and Vascular Tissue Patterning in Arabidopsis

Supplementary Figure Legend

Supplementary Figure 1. Microarray analysis of the YUC gene expression. Microarray data from multiple Affymetrix chip experiments were extracted from the public databases

(https://www.genevestigator.ethz.ch) and re-plotted.

Supplementary Figure 2. Analysis of promoter::GUS lines of YUC1, YUC2, and YUC4. A)

GUS staining of a five-day old YUC1::GUS seedling. B) GUS staining of a five-day old

YUC2::GUS seedling. C) GUS staining of a five-day old YUC4::GUS seedling. D) GUS staining of a fifteen-day old YUC1::GUS seedling. E) GUS staining of a fifteen-day old

YUC2::GUS seedling. F) GUS staining of a fifteen-day old YUC4::GUS seedling.

Supplementary Figure 3. RT-PCR analysis of the T-DNA yuc mutants. Total RNA samples from the indicated yuc mutants were used for the RT-PCR analysis. AtCAND1 was used as a control. Lane 1, molecular weight markers; lane 2and 3, PCR with YUC1 primers; lane 4 and 5,

YUC2 primers; lane 6, 7, and 8, YUC4 primers; lane 9 and 10, YUC6 primers

1 Supplementary Table 1. Quantitative analysis of yuc1yuc4 flowers yuc1yuc Sepal Petal- Stamen Gyneocium without Gyneocium with Unknown 4 flowers -like like -like meristem-like tissue meristem-like tissue organs 1 1 0 2 1 0 0 2 0 3 1 0 1 0 3 1 0 0 0 1 0 4 1 0 0 0 1 0 4 0 1 0 1 0 2 6 1 0 1 1 0 0 7 0 0 0 1 0 1 8 0 3 0 0 1 0 9 2 1 0 1 0 2 10 0 0 0 1 0 1 11 0 0 2 0 1 0 12 2 0 2 0 1 0 13 0 0 4 3 0 0 14 1 2 0 1 0 0 15 0 3 0 1 0 0 16 0 1 0 0 1 0 17 0 0 2 1 0 0 18 1 0 2 1 0 1 19 1 0 1 0 1 0 20 0 0 2 1 0 0 Average 0.55 0.7 0.95 0.7 0.4 0.35

Supplemental Table 2. Analysis of flowers of yuc1yuc4 transformed with the YUC1::iaaM construct. iaaM in sepal petal stamen Gyneocium Gyneocuim with Unknown organs yuc1yuc without meristem- meristem-like 4 like tissue tissue 1 4 3 6 1 0 0 2 4 2 5 1 0 0 3 4 2 5 1 0 0 4 4 4 4 1 0 0 5 4 2 6 1 0 0 6 4 1 6 1 0 0 7 4 2 4 1 0 0 8 4 3 5 1 0 0 9 4 2 5 1 0 0 10 4 3 4 1 0 0 Average 4 2.4 5 1 0 0