De Novo Auxin Biosynthesis Is Necessary for Flower and Vascular Tissue Patterning in Arabidopsis

De Novo Auxin Biosynthesis Is Necessary for Flower and Vascular Tissue Patterning in Arabidopsis

<p>Supplementary Figure Legend</p><p>Supplementary Figure 1. Microarray analysis of the YUC gene expression. Microarray data from multiple Affymetrix chip experiments were extracted from the public databases </p><p>(https://www.genevestigator.ethz.ch) and re-plotted. </p><p>Supplementary Figure 2. Analysis of promoter::GUS lines of YUC1, YUC2, and YUC4. A) </p><p>GUS staining of a five-day old YUC1::GUS seedling. B) GUS staining of a five-day old </p><p>YUC2::GUS seedling. C) GUS staining of a five-day old YUC4::GUS seedling. D) GUS staining of a fifteen-day old YUC1::GUS seedling. E) GUS staining of a fifteen-day old </p><p>YUC2::GUS seedling. F) GUS staining of a fifteen-day old YUC4::GUS seedling.</p><p>Supplementary Figure 3. RT-PCR analysis of the T-DNA yuc mutants. Total RNA samples from the indicated yuc mutants were used for the RT-PCR analysis. AtCAND1 was used as a control. Lane 1, molecular weight markers; lane 2and 3, PCR with YUC1 primers; lane 4 and 5, </p><p>YUC2 primers; lane 6, 7, and 8, YUC4 primers; lane 9 and 10, YUC6 primers</p><p>1 Supplementary Table 1. Quantitative analysis of yuc1yuc4 flowers yuc1yuc Sepal Petal- Stamen Gyneocium without Gyneocium with Unknown 4 flowers -like like -like meristem-like tissue meristem-like tissue organs 1 1 0 2 1 0 0 2 0 3 1 0 1 0 3 1 0 0 0 1 0 4 1 0 0 0 1 0 4 0 1 0 1 0 2 6 1 0 1 1 0 0 7 0 0 0 1 0 1 8 0 3 0 0 1 0 9 2 1 0 1 0 2 10 0 0 0 1 0 1 11 0 0 2 0 1 0 12 2 0 2 0 1 0 13 0 0 4 3 0 0 14 1 2 0 1 0 0 15 0 3 0 1 0 0 16 0 1 0 0 1 0 17 0 0 2 1 0 0 18 1 0 2 1 0 1 19 1 0 1 0 1 0 20 0 0 2 1 0 0 Average 0.55 0.7 0.95 0.7 0.4 0.35</p><p>Supplemental Table 2. Analysis of flowers of yuc1yuc4 transformed with the YUC1::iaaM construct. iaaM in sepal petal stamen Gyneocium Gyneocuim with Unknown organs yuc1yuc without meristem- meristem-like 4 like tissue tissue 1 4 3 6 1 0 0 2 4 2 5 1 0 0 3 4 2 5 1 0 0 4 4 4 4 1 0 0 5 4 2 6 1 0 0 6 4 1 6 1 0 0 7 4 2 4 1 0 0 8 4 3 5 1 0 0 9 4 2 5 1 0 0 10 4 3 4 1 0 0 Average 4 2.4 5 1 0 0</p><p>2 3</p>

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