Introduction to Laboratory Research

Montgomery County Campus A&R 210 Monday thru Friday 6/29 – 7/10 10:45 – 1:45 pm Larissa Diaz Syllabus subject to change

This is an exciting time to work in biotechnology research. Basic research findings are being applied to medicine, agriculture, and the environment; and a variety of new biotechnology products are moving into the product development pipeline. Behind each of these accomplishments lies extensive laboratory research. In this class, you will explore a variety of experimental techniques and evaluate their roles in modern biotechnology research. These include DNA purification and manipulation, polymerase chain reaction (PCR), and an introduction to applications of bioinformatics software in the laboratory setting. You will also have the opportunity to visit a biotechnology or biomedical laboratory focused on current and cutting edge research.

Learning Objectives:

Upon completion of this course, students will be able to: 1.) Explain the theory behind several biochemical and molecular biological laboratory techniques. 2.) Perform and evaluate a variety of laboratory techniques and their role in biological and biomedical research. 3.) Analyze and explain research currently being performed in biomedical laboratories.

Student Evaluation:

This course will consist of five evaluated areas. Grades are as follows (A 90-100%, B 80-89%, C 70-79%, D 60-69%, F < 60%. Plusses and minuses will also be used.

Laboratory Notebook -25% of final grade

A laboratory notebook will be kept and students will record each experiment. A guide to the laboratory notebook will be provided the first day of class.

Quizzes 25% of final grade

There will be two quizzes, one on Monday, July 6th and one on Friday July 10nd.

Lab tour summaries - 25% of final grade

Laboratory visits will be followed by written summaries and class discussions of the laboratory focus and research. Daily homework assignments 25% of grade Students will have questions to answer each day for homework that are due the following day.

Attendance policy

Students are expected to attend every class meeting and activity. Excused absences are allowed for illness and family emergencies. Please notify me as far in advance as possible. You are still responsible for making up missed work. Any unexcused absence will lower your class participation grade by a full letter grade.

Johns Hopkins Policies and Support Services

This course is governed by the policies set forth in the Johns Hopkins University Undergraduate Student Handbook. Some JHU student support services you may find useful include:

Support Service Location Phone number/Website Library E- http://www.library.jhu.edu/services/forms/reserves.htm [email protected] services l

Summer & 3505 N. Charles Street, Suite 101 410-516-4548 Intersession www.jhu.edu/ Programs summer

Classroom Accommodations for Students with Disabilities

If you are a student with a documented disability who requires academic accommodations, please contact The Office of Student Disability Services at [email protected], call 410-5164720, or visit 385 Garland Hall.

Statement of Diversity and Inclusion

Johns Hopkins University is a community committed to sharing values of diversity and inclusion in order to achieve and sustain excellence. We believe excellence is best promoted by being a diverse group of students, faculty, and staff who are committed to creating a climate of mutual respect that is supportive of one another's success. Through its curricula and clinical experiences, we support the University's goal of diversity, working toward an ultimate outcome of best serving the needs of the students. Faculty and staff are expected to demonstrate an understanding of diversity as it relates to planning, instruction, management, and assessment.

Ethics The strength of the university depends on academic and personal integrity. In this course, you must be honest and truthful. Ethical violations include cheating on exams, plagiarism, reuse of assignments, improper use of the Internet and electronic devises, unauthorized collaboration, alteration of graded assignments, forgery and falsification, lying, facilitating academic dishonesty, and unfair competition. Report any violations you witness to the instructor. Course Schedule (subject to change)

Day Morning Session 1 Morning Session Lecture Other quizzes Lecture 2 r methods/lab Lab tours 6/29 Lecture: Class Laboratory Larissa Introduction Safety and Lab Diaz Molecular biology Math Practice pipetting, solution preparation, (%, molar, etc) 6/30 Lecture: What is Extraction of plant Larissa DNA? DNA , Diaz (chromosomal Quantification and DNA, plasmid, purity of DNA RNA)

7/1 Lecture: DNA PCR amplification Larissa replication and PCR of Gene Diaz 7/2 Lecture: Restriction PCR amplification Larissa Restriction Enzyme Analysis of Gene, Plasmid Diaz Enzyme purification Analysis of plasmid DNA 7/6 Lecture: Ligation into Larissa Quiz 1 Extrachromosomal plasmid, Diaz DNA (Plasmid, Transformation plastids, bacterial) 7/7 Lecture: Grow up plasmid Larissa Lab Tour Transformation, DNA Diaz transfection & electroporation 7/8 Lecture: Mini-preps, Gel Larissa Electrophoresis electrophoresis Diaz 7/9 Lecture: computer Bfx analysis of Larissa biology (Bfx) plant DNA Diaz 7/10 Class Review Quiz Quiz 2