DOCSLIB.ORG

The Intestinal Stem Cell

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Intestinal Stem Cell Downloaded from genesdev.cshlp.org on September 29, 2021 - Published by Cold Spring Harbor Laboratory Press REVIEW The intestinal stem cell Nick Barker, Marc van de Wetering, and Hans Clevers1 Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, the Netherlands The epithelium of the adult mammalian intestine is in a Claudinot et al. 2005). Of note, multipotency can only be constant dialog with its underlying mesenchyme to di- definitively demonstrated when transplantation can be rect progenitor proliferation, lineage commitment, ter- performed with a single cell, which is rarely possible. As minal differentiation, and, ultimately, cell death. The an alternative strategy, candidate stem cells are geneti- epithelium is shaped into spatially distinct compart- cally marked in situ, after which the introduced marker ments that are dedicated to each of these events. While allows the visualization of the modified stem cell and its the intestinal epithelium represents the most vigorously clonal offspring over time. As an example of the latter renewing adult tissue in mammals, the stem cells that approach, a progesterone-responsive version of the Cre fuel this self-renewal process have been identified only recombinase enzyme was specifically expressed in cells recently. The unique epithelial anatomy makes the in- residing in the bulge region of hair follicles using a trans- testinal crypt one of the most accessible models for the genic Keratin-15 promoter (Morris et al. 2004). Activa- study of adult stem cell biology. This review attempts to tion of the Cre enzyme by progesterone irreversibly ac- provide a comprehensive overview of four decades of re- tivated the genetic marker R26R-LacZ in the bulge cells. search on crypt stem cells. A simple blue stain for LacZ activity revealed that over time the entire hair follicle and its associated hair de- rived from the marked bulge stem cells, thus establish- Adult tissue stem cells can be defined by two essential ing that the bulge cells are endowed with longevity and features. First, a population of tissue stem cells should be pluripotency. maintained over long periods of time, often the entire Genetic marking is often technically challenging, as lifetime of the organism (“longevity”). And second, these one needs to be able to genetically modify individual long-lived stem cell populations should be able to gen- stem cells. Sometimes a combination of the two ap- erate the differentiated cell types of the tissue. Most proaches is applied, particularly when single cell trans- adult stem cells can generate multiple cell types (“mul- plantation is not feasible and in situ marking of indi- tipotency”), yet examples also exist in which they only vidual cells cannot be achieved. In such cases, one can generate offspring of a single lineage. Two experimental genetically mark individual cells in enriched stem cell strategies can unveil these combined features in candi- populations; for instance, by retroviral integration date stem cell populations. In this review, these are ar- (Jordan and Lemischka 1990) or by titration of individual bitrarily termed the transplantation strategy and the ge- putative stem cells into a stem cell suspension of a netic marking strategy. The transplantation strategy uti- different genetic background prior to transplantation lizes molecular markers to enrich putative stem cell (Smith et al. 1991). populations, followed by in vitro culture and/or trans- plantation into recipient animals. This approach has been highly successful in the identification of the hema- Quiescence and asymmetric cell division topoietic stem cell from bone marrow (Spangrude et al. In addition to longevity and multipotency, stem cells are 1988) and—more recently—of cancer stem cells in leu- often tacitly assumed to be endowed with two other kemias (Bonnet and Dick 1997) and in solid tumors characteristics. Stem cells are commonly believed to di- (Singh et al. 2004; Dalerba et al. 2007; O’Brien et al. 2007; vide very infrequently (“quiescence”), and when this oc- Prince et al. 2007). In an elegant example, Visvader and curs, to generate one rapidly cycling daughter cell, while colleagues regrew an entire mammary gland (Shackleton the other daughter replaces the parent stem cell (“asym- et al. 2006) from a single isolated stem cell. Similarly, metric cell division”). The rapidly cycling daughter cells, clonal analyses have shown that cells derived from a also called transit-amplifying (TA) cells, are responsible single follicle stem cell can give rise to epidermis, seba- for building tissue mass. TA cells typically undergo a ceous gland, and hair follicles (Blanpain et al. 2004; limited number of cell divisions, after which they termi- nally differentiate. While good examples exist of quiescent stem cells [Keywords: Crypt base columnar cell; intestine; stem cell] (e.g., in the bulge of the hair follicle) (Tumbar et al. 2004), 1Corresponding author. E-MAIL [email protected]; FAX 31-30-2121801. there is actually no a priori reason why stem cells should Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1674008. be quiescent. The best studied animal stem cells, the 1856 GENES & DEVELOPMENT 22:1856–1864 © 2008 by Cold Spring Harbor Laboratory Press ISSN 0890-9369/08; www.genesdev.org Downloaded from genesdev.cshlp.org on September 29, 2021 - Published by Cold Spring Harbor Laboratory Press Identifying the intestinal stem cell germ cells of Drosophila, are actively cycling (Spradling structure. The outer layer consists of several sheets of et al. 2001). Furthermore, while the size of stem cell smooth muscle that, together with the intramural en- populations should be stable over time, there is no logi- teric nervous system, execute the rhythmic peristaltic cal reason why this should be accomplished at the single movements of the intestine. The space between the stem cell level, i.e., by obligatory asymmetric cell divi- outer muscle and the inner epithelial layer is filled by sion (Kiel et al. 2007, and refs therein). Physically defined connective tissue (“stroma”) that contains numerous stem cell niches could maintain stable stem cell popu- blood and lymph vessels, nerve fibers, and various cells lations, while allowing individual stem cells to take any of the immune system. On the inside, the luminal sur- of three courses in mitosis: the generation of two stem face consists of a simple epithelium; a single-cell layer cells, of two daughter cells, or of one of each. Of note, the termed the mucosa. The mucosa is responsible for the champion of all stem cells, the Embryonic Stem (ES) cell, processing and absorption of nutrients as well as for the is a very rapidly cycling cell that never undergoes asym- compaction of the stool (Sancho et al. 2003). metric cell divisions, at least when kept under appropri- The intestinal tract can be anatomically divided into ate culture conditions. two well-defined segments: the small intestine and the large intestine or colon. The small intestine is subdi- Surrogate markers for stemness: DNA label retention vided into three proximal-distal segments: the duode- num, jejunum, and ileum. The absorptive surface area of In many tissues, the technology is not available to iso- the small intestine is dramatically increased by numer- late/transplant or to genetically mark adult stem cells. In ous finger-like protrusions that point toward the lumen, such cases, markers known to identify stem cells in un- the so-called villi, and invaginations into the submucosa related tissues, such as CD34, cKit, or the Hoechst dye- known as the crypts of Lieberkühn. The mucosa of the defined Side Population may be used as surrogate stem large intestine lacks villi; crypts invaginate deep into the cell markers (Natarajan and FitzGerald 2007). It is im- submucosa. mediately apparent that this approach should be applied Four differentiated cell types mediate the functions of with great caution. Long-term retention of DNA repre- the intestinal epithelium (Sancho et al. 2003): absorp- sents another commonly used surrogate marker of tive, enteroendocrine, mucosecreting, and Paneth cells. “stemness” (Kiel et al. 2007). An elegant variation of this The relative abundance of each of these three cell types strategy involves the use of Histone-GFP marking (Tum- varies markedly within different segments of the intes- bar et al. 2004). Stem cells are commonly believed to be tine. Absorptive cells (also called enterocytes) are the unique in their ability to retain DNA labels such as BrdU more abundant cell type in the small intestine and are incorporated into their genome during periods of mitotic responsible for the absorption of nutrients from the food activity stimulated, for example, by severe tissue injury. and for the secretion of a cocktail of hydrolytic enzymes This is based on the assumption that stem cells tend to into the lumen. Numbers of mucosecreting cells (also be quiescent, while their direct descendants, the vigor- called goblet cells) increase from proximal (small intes- ously proliferating TA cells, rapidly dilute out any incor- tine) to distal (colon and rectum) as the stool becomes porated DNA label. increasingly compacted. Enteroendocrine cells represent As an alternative mechanism behind DNA label reten- a small proportion (<1%) of the cells in the epithelium. tion, John Cairns formulated the “immortal strand hy- They control gut physiology by secreting a variety of pothesis” 35 years ago (Cairns 1975). Stem cells may hormones including serotonine, substance P, and secre- selectively retain their old DNA strands, while donating tin. Multiple subtypes can be defined by the specific in- the newly synthesized DNA strands to their TA daugh- testinal hormones that are produced (Schonhoff et al. ters. To this day, the immortal strand hypothesis has 2004). Paneth cells reside at the bottom-most positions been the subject of controversy (Lansdorp 2007; Rando of the crypts of the small intestine. They secrete antimi- 2007). Few convincing examples have been reported, crobial agents such as cryptidins (termed defensins in while its molecular machinery remains unidentified.
Load more

Recommended publications
  • A Comparative Study of the Ultrastructure of Microvilli in the Epithelium of Small and Large Intestine of Mice
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central A COMPARATIVE STUDY OF THE ULTRASTRUCTURE OF MICROVILLI IN THE EPITHELIUM OF SMALL AND LARGE INTESTINE OF MICE T. M. MUKHERJEE and A. WYNN WILLIAMS From the Electron Microscope Laboratory, the Departlnent of Pathology, the University of Otago Medical School, Dunedin, New Zealand ABSTRACT A comparative analysis of the fine structure of the microvilli on jejunal and colonic epi- thelial cells of the mouse intestine has been made. The microvilli in these two locations demonstrate a remarkably similar fine structure with respect to the thickness of the plasma membrane, the extent of the filament-free zone, and the characteristics of the microfila- ments situated within the microvillous core. Some of the core microfilaments appear to continue across the plasma membrane limiting the tip of the microvillus. The main differ- ence between the microvilli of small intestine and colon is in the extent and organization of the surface coat. In the small intestine, in addition to the commonly observed thin surface "fuzz," occasional areas of the jejunal villus show a more conspicuous surface coat covering the tips of the microvilli. Evidence has been put forward which indicates that the surface coat is an integral part of the epithelial cells. In contrast to the jejunal epithelium, the colonic epithelium is endowed with a thicker surface coat. Variations in the organization of the surface coat at different levels of the colonic crypts have also been noted. The func- tional significance of these variations in the surface coat is discussed.
    [Show full text]
  • Gastrointestinal Stem Cells in Health and Disease: from Flies to Humans Hongjie Li1,2 and Heinrich Jasper1,*
    © 2016. Published by The Company of Biologists Ltd | Disease Models & Mechanisms (2016) 9, 487-499 doi:10.1242/dmm.024232 REVIEW SUBJECT COLLECTION: TRANSLATIONAL IMPACT OF DROSOPHILA Gastrointestinal stem cells in health and disease: from flies to humans Hongjie Li1,2 and Heinrich Jasper1,* ABSTRACT is Barrett’s metaplasia (see Box 1), in which the esophageal The gastrointestinal tract of complex metazoans is highly squamous epithelium acquires properties that are reminiscent of the compartmentalized. It is lined by a series of specialized epithelia gastric or intestinal columnar epithelium. This transformation has that are regenerated by specific populations of stem cells. To maintain been associated with acid reflux disease and is believed to be a cause tissue homeostasis, the proliferative activity of stem and/or progenitor of esophageal adenocarcinomas (Falk, 2002; Hvid-Jensen et al., cells has to be carefully controlled and coordinated with regionally 2011). Dysplasia (see Box 1), another type of epithelial lesion that distinct programs of differentiation. Metaplasias and dysplasias, commonly affects the human GI tract, is characterized by aberrant precancerous lesions that commonly occur in the human cell proliferation and differentiation. Dysplastic changes are often gastrointestinal tract, are often associated with the aberrant found at later stages during epithelial carcinogenesis than are proliferation and differentiation of stem and/or progenitor cells. The metaplasias, and eventually lead to invasive carcinoma (see Box 1) increasingly sophisticated characterization of stem cells in the (Correa and Houghton, 2007; Ullman et al., 2009). Much remains to gastrointestinal tract of mammals and of the fruit fly Drosophila has be learnt about intestinal metaplasias and dysplasias, not least provided important new insights into these processes and into the because of their clinical significance, such as the exact cellular mechanisms that drive epithelial dysfunction.
    [Show full text]
  • Study of Mucin Turnover in the Small Intestine by in Vivo Labeling Hannah Schneider, Thaher Pelaseyed, Frida Svensson & Malin E
    www.nature.com/scientificreports OPEN Study of mucin turnover in the small intestine by in vivo labeling Hannah Schneider, Thaher Pelaseyed, Frida Svensson & Malin E. V. Johansson Mucins are highly glycosylated proteins which protect the epithelium. In the small intestine, the goblet Received: 23 January 2018 cell-secreted Muc2 mucin constitutes the main component of the loose mucus layer that traps luminal Accepted: 26 March 2018 material. The transmembrane mucin Muc17 forms part of the carbohydrate-rich glycocalyx covering Published: xx xx xxxx intestinal epithelial cells. Our study aimed at investigating the turnover of these mucins in the small intestine by using in vivo labeling of O-glycans with N-azidoacetylgalactosamine. Mice were injected intraperitoneally and sacrifced every hour up to 12 hours and at 24 hours. Samples were fxed with preservation of the mucus layer and stained for Muc2 and Muc17. Turnover of Muc2 was slower in goblet cells of the crypts compared to goblet cells along the villi. Muc17 showed stable expression over time at the plasma membrane on villi tips, in crypts and at crypt openings. In conclusion, we have identifed diferent subtypes of goblet cells based on their rate of mucin biosynthesis and secretion. In order to protect the intestinal epithelium from chemical and bacterial hazards, fast and frequent renewal of the secreted mucus layer in the villi area is combined with massive secretion of stored Muc2 from goblet cells in the upper crypt. Te small intestinal epithelium is constantly aiming to balance efective nutritional uptake with minimal damage due to exposure to ingested, secreted and resident agents.
    [Show full text]
  • Nanoarchitecture and Dynamics of the Mouse Enteric Glycocalyx Examined by Freeze-Etching Electron Tomography and Intravital Microscopy
    ARTICLE https://doi.org/10.1038/s42003-019-0735-5 OPEN Nanoarchitecture and dynamics of the mouse enteric glycocalyx examined by freeze-etching electron tomography and intravital microscopy Willy W. Sun1,2,5, Evan S. Krystofiak1,5, Alejandra Leo-Macias1, Runjia Cui1, Antonio Sesso3, Roberto Weigert 4, 1234567890():,; Seham Ebrahim4 & Bechara Kachar 1* The glycocalyx is a highly hydrated, glycoprotein-rich coat shrouding many eukaryotic and prokaryotic cells. The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption. Its disruption has been implicated in numerous gastrointestinal diseases. Yet, due to chal- lenges in preserving and visualizing its native organization, glycocalyx structure-function relationships remain unclear. Here, we characterize the nanoarchitecture of the murine enteric glycocalyx using freeze-etching and electron tomography. Micrometer-long mucin filaments emerge from microvillar-tips and, through zigzagged lateral interactions form a three-dimensional columnar network with a 30 nm mesh. Filament-termini converge into globular structures ~30 nm apart that are liquid-crystalline packed within a single plane. Finally, we assess glycocalyx deformability and porosity using intravital microscopy. We argue that the columnar network architecture and the liquid-crystalline packing of the fila- ment termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents. 1 Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA. 2 Neuroscience and Cognitive Science Program, University of Maryland, College Park, MD 20740, USA. 3 Sector of Structural Biology, Institute of Tropical Medicine, University of São Paulo, Sao Paulo, SP 05403, Brazil.
    [Show full text]
  • Intestinal Organoids Generated from Human Pluripotent Stem Cells
    DOI: 10.31662/jmaj.2019-0027 https://www.jmaj.jp/ Review Article Intestinal Organoids Generated from Human Pluripotent Stem Cells Satoru Tsuruta1),2), Hajime Uchida3), and Hidenori Akutsu2) Abstract: The gastrointestinal system is one of the most complex organ systems in the human body, and consists of numerous cell types originating from three germ layers. To understand intestinal development and homeostasis and elucidate the patho- genesis of intestinal disorders, including unidentified diseases, several in vitro models have been developed. Human pluripo- tent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have remarkable developmental plasticity and possess the potential for a wide variety of applications. Three-dimensional organs, termed organoids and produced in vitro by PSCs, contain not only epithelium but also mesenchymal tissue and partially recapitu- late intestinal functions. Such intestinal organoids have begun to be applied in disease models and drug development and have contributed to a detailed analysis of molecular interactions and findings in the synergistic development of biomedicine for human digestive organs. In this review, we describe gastrointestinal organoid technology derived from PSCs and consid- er its potential applications. Key Words: intestinal organoids, embryonic stem cells, induced pluripotent stem cells, gastrointestinal disease, drug discovery Introduction the anterior to posterior axis. Accompanied by the develop- ment of the fetus, repeated gut
    [Show full text]
  • Regulation of Colonic Epithelial Cell Homeostasis by Mtorc1
    www.nature.com/scientificreports OPEN Regulation of colonic epithelial cell homeostasis by mTORC1 Takenori Kotani1, Jajar Setiawan1,2, Tasuku Konno1, Noriko Ihara1, Saki Okamoto1, Yasuyuki Saito1, Yoji Murata1, Tetsuo Noda3 & Takashi Matozaki1* Cell signaling important for homeostatic regulation of colonic epithelial cells (CECs) remains poorly understood. Mammalian target of rapamycin complex 1 (mTORC1), a protein complex that contains the serine-threonine kinase mTOR, mediates signaling that underlies the control of cellular functions such as proliferation and autophagy by various external stimuli. We here show that ablation of tuberous sclerosis complex 2 (Tsc2), a negative regulator of mTORC1, specifcally in intestinal epithelial cells of mice resulted in increased activity of mTORC1 of, as well as increased proliferative activity of, CECs. Such Tsc2 ablation also reduced the population of Lgr5-positive colonic stem cells and the expression of Wnt target genes in CECs. The stimulatory phosphorylation of the kinase Akt and inhibitory phosphorylation of glycogen synthase kinase 3β were both markedly decreased in the colon of the Tsc2 conditional knockout (CKO) mice. Development of colonic organoids with cryptlike structures was enhanced for Tsc2 CKO mice compared with control mice. Finally, Tsc2 CKO mice manifested increased susceptibility to dextran sulfate sodium–induced colitis. Our results thus suggest that mTORC1 activity promotes the proliferation of, as well as the expression of Wnt target genes in, CECs and thereby contributes to colonic organogenesis and homeostasis. Both the small intestine and colon of mammals are important for food digestion and the absorption of nutrients, water, and electrolytes. Te intestinal epithelium, in particular, plays a key role in these functions, although the structure and cell components of the epithelium difer between the small intestine and colon.
    [Show full text]
  • Correlation of Ultrasonographic Small Intestinal Wall Layering with Histology in Normal Dogs
    Louisiana State University LSU Digital Commons LSU Master's Theses Graduate School 2015 Correlation of Ultrasonographic Small Intestinal Wall Layering with Histology in Normal Dogs Alexandre Benjamin Le Roux Louisiana State University and Agricultural and Mechanical College, [email protected] Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_theses Part of the Veterinary Medicine Commons Recommended Citation Le Roux, Alexandre Benjamin, "Correlation of Ultrasonographic Small Intestinal Wall Layering with Histology in Normal Dogs" (2015). LSU Master's Theses. 1148. https://digitalcommons.lsu.edu/gradschool_theses/1148 This Thesis is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Master's Theses by an authorized graduate school editor of LSU Digital Commons. For more information, please contact [email protected]. CORRELATION OF ULTRASONOGRAPHIC SMALL INTESTINAL WALL LAYERING WITH HISTOLOGY IN NORMAL DOGS A Thesis Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Masters of Science in The School of Veterinary Medicine through The Department of Veterinary Clinical Sciences by Alexandre Benjamin Le Roux DrMedVet, Ecole Nationale Vétérinaire de Nantes, 2006 May 2015 To my parents, my family and all my friends, for their continuous support… ii ACKNOWLEDGMENTS Foremost, I would like to express my deepest gratitude to the members of my committee, Drs. Lorrie Gaschen, Frederic Gaschen, Abbigail Granger and Nathalie Rademacher for the continuous support and guidance that they gave me through my residency and Master program research, as well as during the preparation of this manuscript.
    [Show full text]
  • Extracellular Matrix Mechanical Properties and Regulation of the Intestinal Stem Cells: When Mechanics Control Fate
    cells Review Extracellular Matrix Mechanical Properties and Regulation of the Intestinal Stem Cells: When Mechanics Control Fate 1, , 1,2, 2 2 Lauriane Onfroy-Roy * y, Dimitri Hamel y, Julie Foncy , Laurent Malaquin and Audrey Ferrand 1,* 1 IRSD, Université de Toulouse, INSERM, INRA, ENVT, UPS, 31024 Toulouse, France; [email protected] 2 LAAS-CNRS, Université de Toulouse, CNRS, 31400 Toulouse, France; [email protected] (J.F.); [email protected] (L.M.) * Correspondence: [email protected] (L.O.-R.); [email protected] (A.F.); Tel.: +33-5-62-744-522 (A.F.) These authors contributed equally to the work. y Received: 31 October 2020; Accepted: 4 December 2020; Published: 7 December 2020 Abstract: Intestinal stem cells (ISC) are crucial players in colon epithelium physiology. The accurate control of their auto-renewal, proliferation and differentiation capacities provides a constant flow of regeneration, maintaining the epithelial intestinal barrier integrity. Under stress conditions, colon epithelium homeostasis in disrupted, evolving towards pathologies such as inflammatory bowel diseases or colorectal cancer. A specific environment, namely the ISC niche constituted by the surrounding mesenchymal stem cells, the factors they secrete and the extracellular matrix (ECM), tightly controls ISC homeostasis. Colon ECM exerts physical constraint on the enclosed stem cells through peculiar topography, stiffness and deformability. However, little is known on the molecular and cellular events involved in ECM regulation of the ISC phenotype and fate. To address this question, combining accurately reproduced colon ECM mechanical parameters to primary ISC cultures such as organoids is an appropriated approach. Here, we review colon ECM physical properties at physiological and pathological states and their bioengineered in vitro reproduction applications to ISC studies.
    [Show full text]
  • Maintenance of Intestinal Homeostasis by Mucosal Barriers Ryu Okumura1,2,3 and Kiyoshi Takeda1,2,3*
    Okumura and Takeda Inflammation and Regeneration (2018) 38:5 Inflammation and Regeneration https://doi.org/10.1186/s41232-018-0063-z REVIEW Open Access Maintenance of intestinal homeostasis by mucosal barriers Ryu Okumura1,2,3 and Kiyoshi Takeda1,2,3* Abstract Background: The intestine is inhabited by a tremendous number of microorganisms, which provide many benefits to nutrition, metabolism and immunity. Mucosal barriers by intestinal epithelial cells make it possible to maintain the symbiotic relationship between the gut microbiota and the host by separating them. Recent evidence indicates that mucosal barrier dysfunction contributes to the development of inflammatory bowel disease (IBD). In this review, we focus on the mechanisms by which mucosal barriers maintain gut homeostasis. Main text: Gut mucosal barriers are classified into chemical and physical barriers. Chemical barriers, including antimicrobial peptides (AMPs), are chemical agents that attack invading microorganisms, and physical barriers, including the mucus layer and the cell junction, are walls that physically repel invading microorganisms. These barriers, which are ingeniously modulated by gut microbiota and host immune cells, spatially segregate gut microbiota and the host immunity to avoid unnecessary immune responses to gut commensal microbes. Therefore, mucosal barrier dysfunction allows gut bacteria to invade gut mucosa, inducing excessive immune responses of the host immune cells, which result in intestinal inflammation. Conclusion: Gut mucosal barriers constructed by intestinal epithelial cells maintain gut homeostasis by segregating gut microbiota and host immune cells. Impaired mucosal barrier function contributes to the development of IBD. However, the mechanism by which the mucosal barrier is regulated by gut microbiota remains unclear. Thus, it should be further elucidated in the future to develop a novel therapeutic approach to IBD by targeting the mucosal barrier.
    [Show full text]
  • Human Intestinal M Cells Exhibit Enterocyte-Like Intermediate Filaments
    54 Gut 1998;42:54–62 Human intestinal M cells exhibit enterocyte-like intermediate filaments Gut: first published as 10.1136/gut.42.1.54 on 1 January 1998. Downloaded from T Kucharzik, N Lügering, K W Schmid, M A Schmidt, R Stoll, W Domschke Abstract antigens.5612 As M cells have a high capacity Background—The derivation and ul- for transcytosis of a wide range of microorgan- trastructural composition of M cells cov- isms and macromolecules, they are believed to ering the lymphoid follicles of Peyer’s act as an antigen sampling system.34 M cells patches is still unknown. Results from dif- can be characterised electron microscopically ferent animal models have shown that by their characteristic morphology, notably there are species specific diVerences in their atypical microvilli and the presence of an the composition of intermediate filaments invagination of the basolateral membrane har- between M cells and neighbouring entero- bouring leucocytes.47Since the first report on cytes. Little is known, however, about M cells in the human ileum1 and appendix,2 intermediate filaments of human M cells. numerous studies have been done to elucidate Aims—To compare components of the their morphology and to investigate functional cytoskeleton of human M cells with those aspects of M cells in diVerent animal species of adjacent absorptive enterocytes. (for review see Trier7). Little is known, Methods—The expression and localisation however, about the function and morphology of diVerent cytokeratins, vimentin, and of M cells in humans. Concerning their desmin in M cells was determined on folli- morphology, human M cells reveal an anasto- cle associated epithelia of human appen- mosing, short, ridgelike network of folds and dix using immunohistochemistry and occasionally short microvilli in strong contrast immunogold electron microscopy.
    [Show full text]
  • 46 Small Intestine
    Small Intestine The small intestine extends between the stomach and colon and is divided into the duodenum, jejunum, and ileum. Although there are minor microscopic differences among these subdivisions, all have the same basic organization as the rest of the digestive tube - mucosa, submucosa, muscularis externa, and serosa or adventitia. The transition from one segment to another is gradual. The proximal 12 inches of its length is generally considered duodenum, the remaining proximal two-fifths jejunum and the distal three-fifths ileum. The small intestine moves chyme from the stomach to the colon and completes the digestive processes by adding enzymes secreted by the intestinal mucosa and accessory glands (liver and pancreas). Its primary function, however, is absorption. Approximately 8 to 9 liters of fluid enters the small intestine on a daily basis. Food and liquid intake represents 1-2 liters of this volume the remainder coming from endogenous sources such as salivary, gastric, intestinal, pancreatic, and biliary secretions. Of this volume 6-7 liters is absorbed in the small intestine with only 1-2 liters entering the colon the majority of which is absorbed at this location. Only as very small amount of fluid is evacuated in the stool. The majority of water is absorbed passively in the gut and is largely dependent on an osmotic gradient. Specializations for Absorption Three specializations - plicae circulares, intestinal villi, and microvilli - markedly increase the surface area of the intestinal mucosa to enhance the absorptive process. It is estimated that these morphological features provide an absorptive surface area of 200 M2. Plicae circulares are large, permanent folds that consist of the intestinal mucosa and a core of submucosa.
    [Show full text]
  • Butyrate and the Intestinal Epithelium: Modulation of Proliferation and Inflammation in Homeostasis and Disease
    cells Review Butyrate and the Intestinal Epithelium: Modulation of Proliferation and Inflammation in Homeostasis and Disease Pooja S. Salvi and Robert A. Cowles * Department of Surgery, Division of Pediatric Surgery, Yale School of Medicine, Yale University, New Haven, CT 065110, USA; [email protected] * Correspondence: [email protected]; Tel.: +1-(203)-785-2701 Abstract: The microbial metabolite butyrate serves as a link between the intestinal microbiome and epithelium. The monocarboxylate transporters MCT1 and SMCT1 are the predominant means of butyrate transport from the intestinal lumen to epithelial cytoplasm, where the molecule undergoes rapid β-oxidation to generate cellular fuel. However, not all epithelial cells metabolize butyrate equally. Undifferentiated colonocytes, including neoplastic cells and intestinal stem cells at the epithe- lial crypt base preferentially utilize glucose over butyrate for cellular fuel. This divergent metabolic conditioning is central to the phenomenon known as “butyrate paradox”, in which butyrate induces contradictory effects on epithelial proliferation in undifferentiated and differentiated colonocytes. There is evidence that accumulation of butyrate in epithelial cells results in histone modification and altered transcriptional activation that halts cell cycle progression. This manifests in the apparent protective effect of butyrate against colonic neoplasia. A corollary to this process is butyrate-induced inhibition of intestinal stem cells. Yet, emerging research has illustrated that the evolution of the crypt, along with butyrate-producing bacteria in the intestine, serve to protect crypt base stem cells from butyrate’s anti-proliferative effects. Butyrate also regulates epithelial inflammation and tolerance to Citation: Salvi, P.S.; Cowles, R.A. antigens, through production of anti-inflammatory cytokines and induction of tolerogenic dendritic Butyrate and the Intestinal cells.
    [Show full text]