Electronic Supplementary Material s37

Electronic Supplementary Material

A Fluorescent Sandwich Assay for Thrombin Using Aptamer Modified Magnetic Beads and Quantum Dots

Xiaofang Wang,1,2 Qiang Zhao1*

1. Research Center for Environmental Science and Engineering, Shanxi University, Taiyuan, China, 030006 2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, China, 030006

* Corresponding author Email: [email protected] Tel: +86-351-7018525; Fax: +86-351-7011011

1 Table S1. Performance comparison of various aptamer-based sandwich assays on solid surface for thrombin with respect to applied aptamers, solid support, reporter, limit of detection (LOD), linear dynamic range (LDR), and complex sample tested Methods Aptamers Solid supports Reporters LOD LDR Complex Ref.

sample

Electrochemistry Apt15 electrode HRP 80 nM NA NA 1

Absorbance Apt15 and Apt29 magnetic beads HRP 10 nM 10 nM-80 nM NA 2

2+ ECL Apt15 and Apt29 GNPs on electrode Ru(bpy)3 10 nM 56 nM-900 nM NA 3

Electrochemistry Apt15 and Apt29 electrode GDH 10 nM 40 nM-100 nM NA 4

Fluorescence Apt15 and Apt29 glass slide europium complex 7.7 nM 10 nM-70 nM serum 5

ECL Apt15 and Apt29 electrode QD 2.7 nM 27 nM-545 nM NA 6

Absorbance Apt15 and Apt29 glass slide GNPs 2 nM 2 nM-167 nM NA 7

Fluorescence Apt15 and Apt29; silica nanoparticle fluorescein 1.1 nM 1.1 nM -40 nM serum 8

Electrochemistry Apt15 and Apt29 magnetic beads ALP 0.45 nM 1 nM-100 nM serum; 9

plasma

Magnetorotation Apt15 and Apt29 microsphere magnetic beads 0.3 nM 1 nM- 20 nM NA 10

Fluorescence Apt15 and Apt29 magnetic beads QD 0.27 nM 2.7 nM-27 nM NA 11

Electrochemistry Apt15 and Apt29 electrode QD 0.14 nM 0.28 nM-7 nM NA 12

Fluorescence Apt15 and Apt29 microtiter plates liposome encapsulated 64 pM 72 pM-500 nM plasma 13

Electrochemistry Apt15 and Apt29 magnetic bead Ferrocenyl hexanethiol 0.06 nM 0.1 nM-5 nM NA 14

loaded silica

nanocapsules

Fluorescence Apt15 and Apt29 magnetic beads QD 0.05 nM 0.05 nM-0.5 nM serum this work

SPR Apt15 gold substrate magnetic nanoparticles 0.02 nM 0.27 nM-27 nM NA 15

ICP MS Apt15 and Apt29 magnetic beads GNPs 10 pM 0.05 nM-10 nM serum 16

Electrochemistry Apt15 electrode QD 1 pM 1 pM-1 nM serum 17

Electrochemistry Apt15 and Apt29 magnetic GNPs 1.4 pM 5.6 pM-1.12nM plasma 18

nanoarticles

Electrochemistry Apt15 electrode Pt Co Nanochain 0.39 pM 1 pM-30 nM NA 19

EIS Apt15 electrode GNPs 100 fM 0.05 nM-35 nM serum 20

Electrochemistry Apt15 and Apt29 magnetic HRP-labeled GNPs 30 fM 0.1 pM-60 pM NA 21

nanoparticles

Electrochmesity Apt15 and Apt29 magnetic beads primer DNA for PCR 5.4 fM 0.3 pM-3 nM NA 22

ECL Apt15 and Apt29 electrode SWNT carrying ECL 3 fM 10 fM-10 pM serum 23

Electrochemistry Apt15 electrode QD 0.55 fM 1 fM-20 fM serum 24

Electrochemistry Apt15 and Apt29 magnetic GNPs 8 aM 75 aM-1.5 fM plasma 25

nanoparticles

ECL, Electrochemiluminescence; ICP MS, Inductively coupled plasma mass spectrometry; EIS, Electrochemical impedance spectroscopy; HRP, horseradish peroxidase; GDH, gluocose

2 dehydrogenase; ALP, alkaline phsphatase; GNPs, gold nanoparticles; SWNT, single wall carbon nanotube; NA, not available

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