IOTest CD278 (ICOS)-APC

PN B36127 – 0.5 mL – Liquid – Clone ISA-3

Analyte Specific Reagent. Analytical and performance characteristics are not established.

2. Hutloff, A., Dittrich, AM., Beier, KC., SPECIFICITY REAGENT CONTENTS Eljaschewitsch, B., Kraft, R., The ICOS molecule (inducible T-cell This antibody is provided in phosphate- Anagnostopoulos, I., Kroczek, RA., costimulator, Inducible Costimulator), also buffered saline, containing 0.1% sodium “ICOS is an inducible T-cell co- known as CD278 antigen (1), belongs to the azide and 2 mg/mL bovine serum albumin. stimulator structurally and functionally CD28 and CTLA-4 cell-surface receptor Concentration: See lot specific Certificate of related to CD28”, 1999, Nature. 21, family and shares 27% and 18% homology Analysis at www.beckmancoulter.com. 397, 263-266. with respectively CD28 and CTLA-4. 3. Haimila, KE., Partanen, JA., The CD278 molecule is a disulfide-linked STATEMENTS OF WARNING 1. This reagent contains 0.1% sodium Holopainen, PM., “Genetic homodimeric T-cell surface glycoprotein of polymorphism of the human ICOS 55-60 kDa whose extracellular region azide. Sodium azide under acid conditions yields hydrazoic acid, an ”, 2002, Immunogenetics, 53, 12, consists of a single immunoglobulin (Ig) 1028-1032. superfamily V-set domain (2). extremely toxic compound. Azide compounds should be flushed with 4. Shwartz, R.H., “It takes more than two This molecule is encoded by a gene located to tango”, 2001, Nature, 409, 31-32. on the 2 (2q33) (3). running water while being discarded. These precautions are recommended 5. Sperling, AI., Bluestone, JA., “ICOS The CD278 molecule plays an important role costimulation: It's not just for TH2 cells in cell-cell signaling, immune responses, to avoid deposits in metal piping in which explosive conditions can anymore”, 2001, Nat. Immunol., 2, 7, and regulation of cell proliferation. 573-574. While CD278 antigen is not constitutively develop. If skin or eye contact occurs, wash excessively with water. 6. Dong, C., Juedes, AE., Temann, UA., expressed on naïve peripheral blood T cells, Shresta, S., Allison, JP., Ruddle, NH., it is highly expressed on unstimulated 2. Specimens, samples and all material coming in contact with them should be Flavell, RA., “ICOS co-stimulatory thymocytes of germinal centers, showing a receptor is essential for T-cell role in differentiation (4). considered potentially infectious and disposed of with proper precautions. activation and function”, 2001, Nature, Upon activation by antigen presenting cells, 4, 409, 97- 101. T cells (CD4 positive cells and CD8 positive 3. Never pipet by mouth and avoid contact of samples with skin and 7. McAdam, AJ., Chang, TT., Lumelsky, cells) express inducible co-stimulatory AE., Greenfield, EA., Boussiotis, VA., receptors, including CD28, CTLA4 and mucous membranes. 4. Do not use antibody beyond the Duke-Cohan, JS., Chernova, T., ICOS (5 – 9). The resulting interaction of Malenkovich, N., Jabs, C., Kuchroo, CD278 (ICOS) with its ligand ICOS-L (- expiration date on the label. 5. Do not expose reagents to strong VK., Ling, V., Collins, M., Sharpe, AH., like molecule present on B cells) participates Freeman, GJ., “Mouse inducible to interleukin production, especially IL-10 during storage or incubation. 6. Avoid microbial contamination of costimulatory molecule (ICOS) and IL4 (10 – 13). expression is enhanced by CD28 The central role of CD278 in the generation reagents or incorrect results might occur. costimulation and regulates and maintenance of humoral immunity has differentiation of CD4+ T cells”, 2000, J also been shown to be important. Upon the 7. Use good laboratory practices when handling this reagent. Immunol., 1, 165, 5035-5040. co-stimulation of T cells through ICOS and 8. Tafuri, A., Shahinian, A., Bladt, F., CD28, ICOS binds its ligand ICOS-L (6, 10) 8. Any change in the physical appearance of the reagents may indicate Yoshinaga, SK., Jordana, M., leading to IgG and IgM secretion as well as Wakeham, A., Boucher, LM., IgE production (4, 14). deterioration and the reagent should not be used. Bouchard, D., Chan, VS., Duncan, G., It is no expressed on resting peripheral T Odermatt, B., Ho, A., Itie, A., Horan, T., cells, B cells, NK cells, STORAGE AND HANDLING CONDITIONS Whoriskey, JS., Pawson, T., monocytes/macrophages, platelets and AND STABILITY Penninger, JM., Ohashi, PS., Mak, granulocytes. This reagent is stable up to the expiration TW., “ICOS is essential for effective T- REAGENT date when stored at 2 – 8°C. Do not freeze. helper-cell responses”, 2001, Nature, 4, No reconstitution is necessary. This 409, 105-109. IOTest CD278 (ICOS)-APC monoclonal antibody may be used directly 9. Janke, M., Witsch, EJ., Mages, HW., Conjugated Antibody from the vial. Bring reagent to 18 – 25°C Hutloff, A., Kroczek, RA., “Eminent role PN B36127 - 0.5 mL - Liquid prior to use. of ICOS costimulation for T cells interacting with plasmacytoid dendritic Clone ISA-3 SELECTED RESEARCH REFERENCES cells”, 2006, Immunology. 118, 3, 353- Isotype IgG1, Mouse 1. Zola, H., Swart, B., Nicholson, I., 360. Immunogen Activated T Lymphocytes Aasted, B., Bensussan, A., Boumsell, 10. Mueller, DL., “T cells: A proliferation of Hybridoma X63 x balb/c L., Buckley, C., Clark, G., Drbal, K., costimulatory molecules”, 2000, Curr Source Ascites fluid or supernatant Engel, P., Hart, D., Horejsí, V., Isacke, Biol., 23, 10, 227-230. of in vitro cultured hybridoma C., Macardle, P., Malavasi, F., Mason, 11. Wassink, L., Vieira, PL., Smits, HH., cells. D., Olive, D., Saalmueller, A., Kingsbury, GA., Coyle, AJ., Purification Affinity chromatography Schlossman, SF., Schwartz-Albiez, R., Kapsenberg, ML., Wierenga, EA., Conjugation Allophycocyanin (APC) Simmons, P., Tedder, TF., Uguccioni, “ICOS expression by activated human Molar Ratio APC / Ig : 0.5 - 1.5 M., Warren, H., “CD molecules 2005: Th cells is enhanced by IL-12 and IL- Fluorescence Excites at 633/638 nm human cell differentiation molecules”, 23: increased ICOS expression Emits at 660 nm 2005, Blood. 1,106, 9, 3123-3126. enhances the effector function of both Th1 and Th2 cells”, 2004, J. Immunol., 1, 173, 3, 1779-1786.

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IOTest CD278 (ICOS)-APC

PN B36127 – 0.5 mL – Liquid – Clone ISA-3

12. Khayyamian, S., Hutloff, A., Buchner, K., Grafe, M., Henn, V., Kroczek, RA., TRADEMARKS Mages, HW., “ICOS-ligand, expressed Beckman Coulter, the stylized logo and on human endothelial cells, IOTest are trademarks of Beckman Coulter, costimulates Th1 and Th2 Inc., and registered with the USPTO. secretion by memory CD4+ T cells”, MANUFACTURED BY : 2002, Proc. Natl. Acad. Sci. U S A., 30, IMMUNOTECH SAS 99, 9, 6198-6203. a Beckman Coulter Company 13. Khoury, SJ., Sayegh, MH., “The roles 130, avenue de Lattre de Tassigny of the new negative T cell costimulatory B.P. 177 - 13276 Marseille Cedex 9 pathways in regulating autoimmunity”, France 2004 Immunity, 20, 5, 529-538. 14. McAdam, AJ., Greenwald, RJ., Levin, For additional information, or if damaged MA., Chernova, T., Malenkovich, N., product is received, call Beckman Coulter Ling, V., Freeman, GJ., Sharpe, AH., Customer Service at 800-526-7694 (USA or “ICOS is critical for CD40-mediated Canada) or contact your local Beckman antibody class switching”, 2001, Coulter Representative. Nature, 4, 409, 6816, 102-105. www.beckmancoulter.com

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