Viewing Aquatic Plants – ALGAE
Purpose: To view various specimens of algae to become more familiar with the diversity of morphology (form) in this broad group of aquatic plants. To understand and identify the process of conjugation in Spirogyra.
Materials: - Light Microscope - Prepared slides : 1. Chlamydomonas, 2. Volvox 3. Spirogyra (vegetative/multiple chloroplasts) 4. Spirogyra Conjugation (vegetative/conjugation)
Procedures: 1. View and sketch several specimens, then make three sketches of “Spirogyra - Vegetative and Conjugation” As listed on p.444 of BIOLOGY – Miller-Levine.
Background Information:
Spirogyra Reproduction
Sexual- CONJUGATION: Exchange of genetic information between two similar cells)
Steps of Conjugation:
1. Two filaments (strands) come in contact with each other.
2. The cells of each strand develop swellings which grow towards each other and eventually touch.
3. The ends of the swellings dissolve, producing a tube leading from 1 cell to the other. The tube is called a Conjugation Tube.
4. The contents of 1 cell pass through the tube and join with the contents of the other cell. The nuclei join forming a zygote.
5. A thick wall forms around the zygote to produce a zygospore. The wall protects the zygote from heat, cold, or dryness. In fair conditions, the zygospore will hatch and form a new filament.
6. The cell wall dissolves, leaving the zygospores to wait for a favorable climate.
Name: ______Date: ______Block: ______
Viewing Aquatic Plants – ALGAE
Purpose: To view various specimens of algae to become more familiar with the diversity of morphology (form) in this broad group of aquatic plants. To understand and identify the process of conjugation in Spirogyra.
Materials: - Light Microscope - Prepared slides : 1. Chlamydomonos 2. Volvox 3. Spirogyra (Vegetative/Multiple Chloroplasts) 4. Spirogyra Conjugation (Vegetative and Conjugation)
Procedures: 1. View and sketch several specimens, then make three sketches of “Spirogyra - Vegetative and Conjugation” As listed on p.444 of BIOLOGY – Miller-Levine.
Background Information:
Spirogyra Reproduction
Sexual- CONJUGATION: Exchange of genetic information between two similar cells)
Steps of Conjugation:
1. Two filaments (strands) line up adjacent to each other and come in contact with each other.
2. The cells of each strand develop swellings (conjugal papillae) which grow toward each other and eventually make contact.
3. The ends of the swellings dissolve, producing a tube leading from 1 cell to the other. The tube is called a Conjugation Tube.
4. The contents of 1 cell pass through the tube and join with the contents of the other cell. The nuclei join forming a Zygote.
5. A thick wall forms around the zygote to produce a protected Zygospore. The wall protects the zygote from heat, cold, or dryness. In favorable conditions, the Zygospore will hatch and form a new filament.
6. The cell wall dissolves, leaving the zygospores to wait for a favorable climate.
Viewing Algae : SKETCHES
Slide #1 – Sketch at least 10 organisms
1. CHLAMYDOMONAS (wm)
Power of Mag : 400X Phylum : ______Morphology : ______Slide #2 – Sketch at least one full colony that is housing at least one daughter colony. 2. VOLVOX (all stages)
Power of Mag : 400X Phylum : ______Morphology : ______Slide #3 – Draw at least one full cell with nucleus, chloroplasts and pyrenoids 3. Spiryogyra (Vegetative/Multiple Chloroplasts)
Power: 400X Phylum : ______Morphology : ______Slide #4 – Sketch at least 4 cells in the formation of two cross pairs, include conjugation tubes, perhaps papillae.
4. Spirogyra (Veg. and Conj.)
Power of Mag : 400X Slide #4 – Draw at least two Zygospores. 5. Zygospores on same slide
Power of Mag: 400X Viewing Algae Lab – Assessment
Sketch Proper Phylum Proper Proper Sketch Detail Morphology Magnification (accuracy/labeling) Chlamydomonas Chlorophyta Unicellular 400 X Did you have at least 5 chlamys? Volvox Colony Chlorophyta Colonial 400 X Did you show and label with Daughter colony vs daughter colony colony? Spirogyra Chlorophyta Filamentous 400 X One cell with Nucleus, (Vegetative) Chloroplast, pyrenoid Spirogyra Chlorophyta 400 X 4 cells with conjugation (Conjugative) tubes or conjugal papillae Spirogyra Chlorophyta 400 X At least two full (Conjugation – Zygospores Zygospores) TOTAL ____/3 ____/3 ____/3 ______/10
TOTAL = ______/19
Viewing Algae Lab – Assessment
Sketch Proper Phylum Proper Proper Sketch Detail Morphology Magnification (accuracy/labeling) Chlamydomonas Chlorophyta Unicellular 400 X Did you have at least 5 chlamys? Volvox Colony Chlorophyta Colonial 400 X Did you show and label with Daughter colony vs daughter colony colony? Spirogyra Chlorophyta Filamentous 400 X One cell with Nucleus, (Vegetative) Chloroplast, pyrenoid Spirogyra Chlorophyta 400 X 4 cells with conjugation (Conjugative) tubes or conjugal papillae Spirogyra Chlorophyta 400 X At least two full (Conjugation – Zygospores Zygospores) TOTAL ____/3 ____/3 ____/3 ______/10 TOTAL = ______/19