Identification of the Cysteine Protease Amb a 11 As a Novel Major Allergen from Short Ragweed

Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed

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Table E1. List of IgE-reactive proteins identified from short ragweed pollen. Excised protein spots were analyzed by nanoESI-Qq-TOF MS/MS and proteins were identified by searching Uniprot/Swiss-Prot, NCBInr databases and translated nucleotide sequences from ragweed pollen (homologous sequences were then obtained by running BLAST against the nrNCBI database). The GSAPGSIDTDPNKDF amino acid sequence (Spot # 14) was obtained by Edman degradation. Numbers refer to Fig 1, A. Accession number (Acc.No.). Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed

Table E2. Summary of the main Amb a 11 LysC-peptides (in-solution digestion) identified by MALDI-MS. Modified amino acid residues are shown in bold type. The monoisotopic mass of each N-glycan is indicated. aThe zymogen consists of 386 amino acids, including 108 additional N-terminal residues. Miscleavage (MisC.). Measured (Meas.). Calculated (Calc.). Carbamidomethyl Cys (CAM). Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed

Table E3. Summary of the main Amb a 11 LysC-peptides (in-solution) identified by nanoESI-Qq-TOF MS/MS. Modified amino acid residues are shown in bold type. The monoisotopic mass of each N-glycan is indicated. aThe zymogen consists of 386 amino acids, including 108 additional N-terminal residues. Miscleavage (MisC.). Measured (Meas.). Calculated (Calc.). Carbamidomethyl Cys (CAM). Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed

Table E4. Summary of the main Amb a 11 tryptic peptides (in-gel digestion) identified by nanoESI-Qq-TOF MS/MS. Modified amino acid residues are shown in bold type. aThe zymogen consists of 386 amino acids, including 108 additional N-terminal residues. Miscleavage (MisC.). Measured (Meas.). Calculated (Calc.). Carbamidomethyl Cys (CAM). Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed