Supplementary Figure 1: Long Term Appearance of Accepted Lung Grafts. (A) Gross Appearance

Supplementary Figure 1: Long term appearance of accepted lung grafts. (a) Gross appearance of Balb/c lung allograft 13 months after transplantation into immunosuppressed (MR1, CTLA4-Ig) B6 recipient (Tx depicts transplanted graft). (b)

Histological appearance of native B6 right lung and (c) BALT in Balb/c allograft 7 months after transplantation into immunosuppressed (MR1, CTLA4-Ig) B6 recipient

(100x). (d) represents a zoomed view of BALT shown in (c) (200x).

Supplementary Figure 2: Appearance of immunosuppressed heart grafts. Two-photon image of dendritic cells in (a) heart explants derived from resting B6 CD11c + EYFP mice and (b) Balb/c heart graft explants 30 days after transplantation into immunosuppressed

(MR1, CTLA4-Ig) B6 CD11c+ EYFP recipients. Scale bar = 20 µm. (c) Immunostaining

(100x) for PNAd for B6 heart grafts 30 days after transplantation into immunosuppressed (MR1, CTLA4-Ig) CBA hosts.

Supplementary Figure 3: PD-L1 expression on dendritic cells in BALT of accepted lung allografts. (a) Flow cytometric analysis of PD-L1 expression on myeloid dendritic cells

(CD11c+CD11bhi) in accepted B6 lung allografts 30 days after transplantation into immunosuppressed (MR1, CTLA4-Ig) CBA recipients (n=3) Shaded histogram depicts staining with isotype control antibody. (b) Immunofluorescent staining showing expression of PD-L1 (red) on CD11c+ dendritic cells (green) within BALT of accepted

Balb/c lung allografts 30 days after transplantation into immunosuppressed (MR1,

CTLA4-Ig) B6 CD11c-EYFP hosts.

1 Supplementary Movie 1: Intravital two-photon imaging of dendritic cells (green) within

BALT in accepted Balb/c lung grafts 30 days after transplantation into immunosuppressed (MR1 250 µg i.p. day 0, CTLA4-Ig 200 µg i.p. day 2) B6 CD11c+

EYFP recipients. Relative time is displayed in hrs:min:sec:msec.

Supplementary Movie 2: Intravital two-photon imaging of adoptively transferred

CMTMR-labeled B6 T lymphocytes (yellow) with dendritic cells (green) within BALT in accepted Balb/c lung grafts 30 days after transplantation into immunosuppressed (MR1

250 µg i.p. day 0, CTLA4-Ig 200 µg i.p. day 2) B6 CD11c + EYFP recipients. Collagen and alveolar macrophages appear blue (second harmonic generation) and blood vessels appear red. Relative time is displayed in hrs:min:sec:msec.

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