Supplementary materials
Histological comparison of kidney tissue following radioembolization with yttrium-90 resin microspheres and embolization with bland microspheres
Supplement Table 1. Summary of renal pathology gross macroscopic findings with 90Y-resin microspheres (active) and bland microspheres at
8–9 weeks
Animal 1 2 3 4 5 6 Active Lower pole scar Nodular Lower pole atrophic Upper pole Small lobulated Pale upper pole
pale upper irregular flattened nodular mid and
and mid lower pole
pole Bland Variegated pale Smooth Smooth surface Smooth Smooth minor Pale areas
with pale with small lobulation thinned cortex
blotchy scars upper and mid
areas pole Supplement Table 2. Microscopic features and morphological changes in each pole at 8-9 weeks
3a. 90Y-resin microspheres (active treatment)
Animal (Implanted activity and location)
1 2 3 4 5 6
0.15 GBq, left 0.2 GBq left upper 0.25 GB,q left 0.3 GBq, right 0.325 GBq left 0.35 GBq left
lower pole pole lower pole upper pole lower pole upper pole Upper Microsphere Scarce individual Many especially in Few, individual Moderate in Moderate Many in vessels,
Pole number, in glomeruli vessels associated with glomeruli and individual and including intima
distribution fibrosis scars aggregates in clusters of medium
vessels arteries Glomeruli No abnormality Fibrinoid necrosis, Focal minimal Fibrinoid No abnormality Prolifertive
sclerosis, fibrosis necrosis and necrosis, changes and
proliferation cellular fibrosis
mesangial
proliferation Reaction None Large wedge shaped Minimal fibrosis Wedge-shaped Moderate Extensive
scar with fibrosis and fibrous scars, subcapsular fibrosis, loss of
atrophy interstitial scars tubules.
fibrosis. Xanthomatous
areas
Mild chronic
inflammation,
intimal
proliferation in
vessels most
marked near
aggregates of
microspheres in
vessels Middle Microsphere Few individual Few Many in clusters Few individual Large Many in clusters
Pole number, in vessels and clusters aggregates in
distribution vessels plus interstitial
aggregates Glomeruli Rare sclerotic, Mild necrosis and Marked necrosis Occasional Marked Proliferative
most normal fibrosis and cellular areas of necrosis sclerosis changes and
proliferation and cellular fibrosis
proliferation Reaction Nil Mild subcapsular Wedge-shaped Mild Large scars with Large scars,
scarring marked fibrosis, inflammation fibrosis, fibrosis,
fibrosis, atrophy around some atrophy, and histiocytes with
assoc with large vessels. Little histiocyte xanthomatous
numerous m/s fibrosis aggregates areas, chronic
inflammation Lower Microsphere Many, mainly Few individual Occasional Few individual, Large Many
Pole number, clusters in vessels diffusely in aggregates in
distribution glomeruli vessels Glomeruli Atropic in scar, No abnormality No abnormality Occasional Marked Segmental
otherwise normal areas of necrosis Sclerosis proliferative
and cellular changes and proliferation fibrosis Reaction Large area of None None Areas of Large wedge- Segmental large
atrophy and subcapsular shaped scars scars
fibrosis; some fibrosis with fibrosis
foreign body and atrophy
reaction Ureter Microsphere Present. Isolated Present occasionally in Present in None Present in Present in
number, plus some muscle only muscle and muscle only muscle and
distribution vascular clusters adventitial intima of artery
vessels. Reaction None None Some multi None Slight chronic Patchy moderate
nucleated cells inflammation chronic
inflammation 3b. Bland microspheres
1 2 3 4 5 6
2.13 million, 2.83 million right upper 3.54 million, 4.25 million, 4.6 million, 4.96 million,
right lower pole pole right lower left upper pole right lower right upper
pole pole pole Upper Microsphere Moderate Occasional individual Few in vessels Marked Moderate Few in vessels
Pole number, numbers in and glomeruli aggregates in aggregates and glomeruli
distribution afferent arterioles vessels and
of glomeruli some glomeruli Glomeruli No abnormality No abnormality No abnormality No abnormality, No abnormality No abnormality
No necrosis Reaction None None None Minor chronic Patchy sub- None
inflammation in capsular
interstitium. No scarring
fibrosis Middle Microsphere Moderate, Moderate individual Occasional Moderate in Aggregates in Moderate
Pole number, diffusely in individual vessels and vessels and aggregates in
distribution glomeruli glomeruli interstitium vessels and assoc with giant glomeruli
cell reaction Glomeruli No abnormality No abnormality No abnormality No abnormality No abnormality No abnormality Reaction None None None None. No Minor None
fibrosis subcapsular
scarring Lower Microsphere Marked clusters Moderate individual Occasional Few in vessels Occasionally in Moderate
Pole number, in vessels individual and glomeruli vessels and aggregates in
distribution glomeruli vessels and
glomeruli Glomeruli No abnormality No abnormality No abnormality No abnormality No abnormality No abnormality Reaction Subcapsular None None None, No Minor None
scarring with fibrosis subcapsular
foreign body scarring
giant cell
reaction Ureter Microspheres Present Few individual Present Few individual, Few individual. Occasionally in Occasionally in
individual in in muscle and adventitial Present in Present in muscle muscle
lamina propria vessels muscle and muscle and muscle adventitial
vessels Reaction None No fibrosis - None Mild chronic None
muscle appears inflammation
normal