YOGI VEMANA UNIVERSITY Department of Environmental Science Kadapa, A.P. - 516003, INDIA
Dr.T.Chandrasekhar Mobile: 9573154641 Assistant Professor Fax: 08562-225419 Principle Investigator, CSIR Project E.mail: [email protected] No: YVU/ENVI/TCS-CSIR/Cont./II year/2013-14 Date: 11-11-2013 Quotations invited
To
Sir,
Sub: Department of Environmental Science - CSIR Project, IInd year grants, - quotations invited for chemicals, glassware, and plasticware -reg.
With references to cited above, you are requested to send your lowest quotations in triplicate in the name of Registrar, Yogi Vemana University, Kadapa-516003, YSR (Dt), A.P., India by quoting the reference YVU/ENVI/TCS-CSIR/Cont./II year/2013-14 towards the supply of the following chemicals, glassware and plasticware (list is enclosed) along with your terms and conditions so as to reach The Registrar office on or before 05-12-2013 in the sealed cover by speed or register post only.
You are also requested to send the catalog if any. Quotation includes unit price, taxes, warranty, validity of quotation and transport etc.
Thanking you
Yours Sincerely
(T. CHANDRASEKHAR)
Chemicals
S.No Name of the chemical Make Catalogue.No Quantity
1 Agarose Himedia MB002-100G 100gm 2 Acrylamide Himedia MB068-25G 25gm 3 Bis acrylamide Himedia MB005-25G 25gm 4 SDS Sodium dodesyl sulphate Himedia MB010-25G 25gm 5 APS Ammonium per sulphate Himedia MB003-25G 25gm 6 TEMED Himedia MB026-100ML 100ml 7 Tris free base Himedia MB029-100G 100gm 8 Tris Hcl Himedia MB030-100G 100gm 9 Tris Himedia MB190-100G 100gm 10 Glycine Himedia MB013-100G 100gm 11 DTT dithiotreitol Himedia MB070-5G 5gm 12 β-mercaptoethanol Himedia MB041-100ML 100ml 13 Bromophenol blue Himedia MB123-5G 5gm 14 Coomassie brilliant blue Himedia MB092-5G 5gm 15 Methanol Himedia MB113-500ML 500ml 16 Iso propanol Himedia AS068-500ML 500ml 17 Glycerol Himedia MB060-500ML 500ml 18 Isobutanol Himedia MB056-500ML 500ml 19 Ponceau S Himedia MB096-25G 25gm 20 NaCl sodium chloride Himedia MB023-500G 500gm 21 NaCl sodium chloride Himedia GRM853-500G 500gm 22 BSA bovine serum albumin Himedia MB083-5G 5gm 23 Triton X 100 Himedia MB031-50ML 50ml 24 KCl potassium chloride Himedia GRM698-500G 500gm
25 Na2HPO4 sodium phosphate dibasic Himedia MB024-250G 250gm anhydrous
26 KH2PO4 Potassium phosphate Himedia MB050-100G 100gm monobasic anhydrous 27 EDTA Himedia MB011-500G 500gm 28 DMSO Dimethyl sulphoxide Himedia MB058-100ML 100ml 29 DAB Tetra amino biphenyl Himedia MB121-1G 1gm tetrahyrochloride 30 CTAB Himedia MB101-100G 100gm 31 Chloroform Himedia MB109-100ML 100ml 32 Iso amyl alcohol Himedia MB091-100ML 100ml 33 HEPES Himedia MB016-25G 25gm 34 RNase A Himedia MB087-100MG 100mg 35 Guanidine HCl Himedia MB014-25G 25gm 36 Proteinase K Himedia MB086-10MG 10mg 37 Sodium acetate Himedia MB048-100G 100gm 38 Potassium acetate Himedia MB042-100G 100gm 39 Ammonium acetate Himedia MB033-100G 100gm 40 Ethidium bromide Himedia MB071-1G 1gm 41 PVP Himedia MB102-100G 100gm 42 NaOH sodium hydroxide Himedia GRM467-500G 500gm 43 Boric acid Himedia GRM1224-500G 500gm 44 TCA Trichloro acetic acid Himedia GRM6274-100G 100gm 45 Urea Himedia MB032-500G 500gm 46 Acetone Himedia AS025-2.5L 2.5lt 47 CHAPS Himedia MB084-1G 1gm
48 K2CO3 Himedia GRM731-500G 500gm 49 Phenol Himedia MB081-100ML 100ml 50 EGTA Himedia MB130-10G 10gm 51 PMSF Phenyl methane sulfonyl Himedia MB144-1G 1gm fluoride 52 PEG Polyethylene glycol Himedia MB148-500G 500gm 53 GSSG oxidized glutathione Himedia TC282-100MG 100mg 54 Gelatin Himedia GRM019-500G 500gm 55 Citric acid Himedia MB174-500G 500gm
56 Na2HPO4.2H2O Sodium phosphate Himedia MB126-500G 500gm dibasic dehydrate 57 NBT Nitrotetrazolium blue chloride Himedia MB107-100MG 100mg 58 L-Methionine Himedia PCT0315-25G 25gm 59 Riboflavin Himedia PCT0214-10G 10gm 60 Glacial acetic acid Himedia AS001-500ML 500ml 61 5-Sulphosalicylic acid dihydrate Himedia GRM7527-250G 250gm 62 Orthophosporic acid Himedia AS011-500ML 500ml 63 Toluene Himedia AS074-500ML 500ml 64 Proline Himedia PCT0317-25G 25gm
65 Na2CO3 Himedia GRM851-500G 500gm 66 Potassium sodium tartarate Himedia GRM1046-500G 500gm tetrahydrate 67 Gamborg B5 Medium Himedia PT017-25L 25lt 68 YEP Broth Himedia M1827-500G 500gm 69 Kanamycin Himedia PCT1105-5G 5gm 70 Rifampicin Himedia CMS1889-5G 5gm 71 Cephotaxime Himedia MB134-10G 10gm 72 Acetosyringone Himedia PCT1301-1G 1gm 73 HiPurATMPlant DNA isolation kit Himedia MB502-50PR 50pr 74 Molecular biology grade water Himedia ML024-10x100ML 500ml 75 Folins reagent Himedia ML059-125ML 125ml 76 Bradford reagent Himedia ML106-100ML 100ml 77 Biuret reagent Himedia ML122-100ML 100ml 78 Blocking buffer Himedia ML044-100ML 100ml 79 Ponceau S stain Himedia ML045-250ML 250ml 80 Luria Bertaini Broth Miller Himedia M1245-500G 500gm 81 2X PCR Taq mixture Himedia MBT061-20R 20r(0.5ml) 82 2X PCR Taq mixture Himedia MBT061-50R 50r(1.25ml) 83 Bam H1 restriction enzyme Himedia MBRE002-250U 250u 84 Hind III restriction enzyme Himedia MBRE003-250U 250u 85 2X Laemmli buffer Himedia ML021-5x6ML 5x6ml 86 1X PBS Himedia ML116-100ML 100ml 87 1X TE buffer Himedia ML060-100ML 100ml 88 1Kb DNA ladder 50µg Merck 612652070501730 1 EA 89 1Kb DNA ladder 100µg Merck 612652071001730 1 EA 90 Protein molecular weight broad Merck 623110475001730 1 EA range 50 lanes 0.5ml 91 Protein molecular weight broad Merck 623110472501730 1 EA range 25 lanes 0.25ml 92 Protein molecular weight lower Merck 623110175001730 1 EA range 50 lanes 0.5ml 93 Bam H1 2000U Merck 610100100021730 1 EA 94 Hind III 4000U Merck 610100600021730 1 EA 95 Red dye PCR Master mix 2X (5x10 Merck 610667800041730 1 EA rxn) 96 CTAB plant DNA extraction kit 10 Merck 612105500011730 1 EA preps 97 EmeraldAmp® MAX PCR Master TaKaRa RR320A Mix 160 rxns 98 TaKaRa Taq™ DNA Polymerase TaKaRa R001A 250 units 99 Premix Taq™ DNA Polymerase TaKaRa R004A (TaKaRa Taq™ Version 2.0) 150 units 100 Nucleospin plasmid-50 TaKaRa 740588 101 Nucleo spin extract -50 TaKaRa 740609 102 Not I restriction enzyme NEB 103 ECL western blotting kit Thermoscientifi c 104 X-ray film 105 Cassette 106 Sterlin 5lt x 10 107 Ethyl alocoholAR Any make 500ml x 10 108 DMSF Fisher / …………… ………… Himedia/ Merck 109 Thiourea Fisher / ………… ………… Himedia/ Merck 110 SB3 Fisher / ………… ………… Himedia/ Merck 111 Phospine Fisher / ………… …………. Himedia/ Merck 112 Pharmalyte Fisher / ………… ………….. Himedia/ Merck
113 H2O2 Fisher / ………. ………. Himedia/ Merck
Glassware S.No Name of the glassware Capacity Make & Cat No.
1 Volumetric flask 50 ml Borosil -5641 2 Volumetric flask 100 ml Borosil-5641 3 Volumetric flask 250 ml Borosil-5641 4 Volumetric flask 500 ml Borosil-5641 5 Volumetric flask 1000 ml Borosil-5641 6 Conical flask 50 ml Borosil-4980 7 Conical flask 100 ml Borosil-4980 8 Conical flask 250 ml Borosil -4980 9 Conical flask 500 ml Borosil -4980 10 Conical flask 1000 ml Borosil -4980 11 Measuring cylinder 10 ml Borosil-3022 12 Measuring cylinder 25 ml Borosil-3022 13 Measuring cylinder 50 ml Borosil-3022 14 Measuring cylinder 100 ml Borosil- 3022 15 Measuring cylinder 250 ml Borosil- 3022 16 Measuring cylinder 500 ml Borosil-3022 17 Measuring cylinder 1000 ml Borosil-3022 18 Pipette 0.1 ml Borosil-7060 19 Pipette 0.5 ml Borosil-7060 20 Pipette 1.0 ml Borosil-7060 21 Pipette 2 ml Borosil-7060 22 Pipette 5 ml Borosil-7060 23 Pipette 10ml Borosil-7060 24 Beaker 25 ml Borosil-1000 25 Beaker 50 ml Borosil-1000 26 Beaker 100 ml Borosil-1000 27 Beaker 250ml Borosil-1000 28 Beaker 500ml Borosil-1000 29 Beaker 1000ml Borosil-1000 30 Amber color Reagent bottle 50ml Borosil-1519 31 Amber color Reagent bottle 100ml Borosil-1519 32 Amber color Reagent bottle 250 ml Borosil-1519 33 Amber color Reagent bottle 500 ml Borosil-1519 34 Reagent bottle 50 ml Borosil-1501 35 Reagent bottle 100 ml Borosil-1501 36 Reagent bottle 250 ml Borosil-1501 37 Reagent bottle 500ml Borosil-1501 38 Reagent bottle 1000 ml Borosil-1501 39 Funnel 65 mm Borosil-6401 40 Funnel 75mm Borosil-6401 41 Funnel 100 mm Borosil-6401 42 Test tubes with rim 25x 150 mm Borosil-9800 43 Rimless test tubes 25x 150 mm Borosil-9800 44 Autoclavable Tissue culture bottles with 250ml …….. polystyrene cap 45 S-line Petridishes 100x15mm Borosil-3165 46 S-line Petridishes 200x30mm Borosil-3165
Plasticware
S.No Name of the item (Plasticware) Capacity Make and catalogue No
1 Amber color wide mouth reagent bottle 30ml Tarsons 581300 2 Amber color wide mouth reagent bottle 60ml Tarsons 581310 3 Amber color wide mouth reagent bottle 500ml Tarsons 581340 4 Test tube baskets 180 x 170 x 160 Tarsons 180020 5 Funnel 35mm Tarsons 630010 6 Micropipette tip 0.2-10µl Tarsons 521000 7 Micropipette tip 2-200 µl Tarsons 521010 8 Micropipette tip 200-1000 µl Tarsons 521020 9 Micro tip box 0.2-10µl Tarsons 524051 10 Micro tip box 2-200 µl Tarsons 524050 11 Micro tip box 200-1000 µl Tarsons 524080 12 Tough tags 24 x 12.7mm Tarsons 500060 13 Tough spots dia 9.5 Tarsons 500080 14 Reversible rack with cover 96 places Tarsons 241010 15 Centrifuge tubes conical bottom 15ml Tarsons 500031 16 Centrifuge tubes conical bottom 50ml Tarsons 500041 17 Centrifuge tubes round bottom 16ml Tarsons 543010 18 Centrifuge tubes round bottom 50ml Tarsons 543020 19 Caps 16ml Tarsons 540010 20 Caps 50ml Tarsons 540020 21 Oak ridge centrifuge tubes 10ml Tarsons 541020 22 Oak ridge centrifuge tubes 30ml Tarsons 541030 23 Oak ridge centrifuge tubes 50ml Tarsons 541040 24 Oak ridge centrifuge tubes 70ml Tarsons 541050 25 Rack for microtube 1.5ml 20 places Tarsons 240010 26 Universal combi rack …… Tarsons 202080 27 Test tube stand 25mm tubes 18 places Tarsons 202040 28 Cryo vial sterile 1.8ml Tarsons 523020 29 Cryo vial sterile 4.5ml Tarsons 523030 30 Cryo coder white …… Tarsons 523110 31 Cryo coder blue …… Tarsons 523120 32 Cryo coder red …… Tarsons 523130 33 Cryo coder yellow …… Tarsons 523140 34 Cryo rack 50 places Tarsons 241050 35 Microtube box 1.5ml 64 places Tarsons 524070 36 Cryobox 1.0 and 1.8ml 81 places Tarsons 524010 37 Cryobox 4.5ml 81 places Tarsons 524030 38 Cryo cube box 1.8ml 100 places Tarsons 202070 39 -20 PCR minicooler 0.2ml 96 places Tarsons 525110 40 Ice tray 600ml Tarsons 525080 41 Float rack 1.5ml 16 places Tarsons 240000 42 Float rack 1.5ml 8 places Tarsons 240040 43 Nitrile gloves Small Tarsons 372010 44 Nitrile gloves Medium Tarsons 372020 45 Hand protector grip …… Tarsons 800000 46 Planton plant tissue culture container 7.5 x 7.5 x 10 cm Tarsons 020080 47 Test tube cap 25mm Tarsons 020070 48 Staining box 12.5 x 12.5 x 5 cm Tarsons 482020 49 PCR tubes flat lid 0.2ml Merck/ Himedia/ Tarsons 50 8 strip PCR tubes with flat cap 0.2ml Merck/ Himedia/ Tarsons 51 96 well non skirted PCR blocks 0.2ml Merck/ Himedia/ Tarsons 52 Caps for 96 well PCR blocks 0.2ml Merck/ Himedia/ Tarsons 53 Phyta jar for plant tissue culture 250ml Himedia PW1125-25NO Round container 54 Phyta jar for plant tissue culture 500ml Himedia PW1143-25NO Square container 55 Sterile syringe driven filters 0.22µm 25mm dia Any make with PES membrane 56 Hidispo Bag – 14 500 no Himedia PW038-500NO Size : 20 (h) x 14 (b) 57 Freeze tag, multicolour dots 1x6000 no Himedia LA937M-1x6000NO 58 Pasture pipettes 1x50 no Himedia PW1206-1x50NO 59 Magnetic bar retrievers 1 no Himedia PW176-1NO 60 Spatula -200 50 no Himedia LA006-50NO 61 Metaloop -SS-2 1x8 no Himedia LA650-1x8NO 62 Stainless steel forceps blunt 8 inches 2 no Himedia LA707-2NO 63 Stainless steel forceps blunt 10 inches 2 no Himedia LA708-2NO 64 Stainless steel forceps blunt 12 inches 2 no Himedia LA767-2NO 65 Stainless steel scalpel holder NO.3 2 no Himedia LA829-2NO 66 Stainless steel scalpel holder NO.4 2 no Himedia LA830-2NO 67 Sterile scalpel blade NO.10 1x100 no Himedia LA768-1x100NO 68 Sterile scalpel blade NO.11 1x100 no Himedia LA769-1x100NO 69 Sterile scalpel blade NO.15 1x100 no Himedia LA770-1x100NO 70 Sterile scalpel blade NO.20 1x100 no Himedia LA771-1x100NO 71 PCR tubes work station Orange colour 1x5 no Himedia CG297-1x5NO 72 Parafilm 1 no HimediaLA017-1NO 73 Buffer capsules ,pH:4.0 10 no Himedia LA405-10NO 74 Buffer capsules ,pH:7.0 10 no Himedia LA406-10NO 75 Buffer capsules ,pH:9.2 10 no Himedia LA407-10NO 76 Stainless steel stand 2 no Himedia LA722-2NO 77 HiIndicator pH paper 1 pk Himedia LA310-1PK 78 Coombs for Electrophoresis NYXTECHNIK Mini and Midi system 79 Glass rods 10 No 80 Glass spreaders 4 No 81 Autoclave gloves 2 sets 82 Autoclave biohazard covers 100 No Small and medium size 83 Pestle and mortar 10 No Small size 84 Watmann no 1 filter papers 1 pk 85 Blotting paper 1reem 86 Spirit lamps glass made 4 No 87 Disposable gloves 300 No 88 Cotton rolls 4 No 89 Tissue rolls 1 Box
Yours Sincerely
(T. CHANDRASEKHAR)