Radiation Safey Level 1A (Rsl - 1A)

ANIMAL STANDARD OPERATING PROCEDURE RADIATION SAFEY LEVEL 1A (RSL - 1A)

Non-terminal studies with “long-lived” radioisotopes Any radioisotopes with long half-lives, such as H-3 and C-14 with concentrations in the animal greater than 0.05 microcuries per gram that are used in animals that are maintained for a period after treatment.

Insert Name of Radioisotopes:

 All treatments performed only in PI’s laboratory.  PI will inform IAS veterinary staff prior to initiating isotope experiments in animals and keep IAS informed about the status of room. This will be accomplished by BOTH room signs and contacting the attending clinical veterinarian directly.  Arrangements must be made for isolation/quarantine housing of animals for the duration of the experiment.  Fresh micro-isolator caging will be used for the animals at the time of administration.  Radiation Sign must be posted on the door of the room in which the animals will be housed while experiments are in progress.  Cages will be labeled with a pink card with the following information: Investigator 1. Name of isotope delivered to animal Responsibility 2. Approximate dose 3. Date of administration 4. Date of bedding change 5. Contact Information  Animal husbandry is performed by PI staff  Cards and door signs must be removed by the researcher once the hazard is no longer present.  Radioactive carcasses are held in a specially designated freezer within the PI’s laboratory for ultimate removal via EH&S.  Consult with the Radiation Safety Officer concerning shielding requirements.  Access limited to PI staff and selected veterinary staff.  All Personal Protective Equipment (PPE) gown, gloves, face mask, shoe covers and cap Entry must be worn to enter animal room. Requirements  Hands must be washed upon exiting animal room.  Surgical mask, goggles, faceshield or use of a Biological Safety Cabinet (BSC) are required when conducting research.  Animal waste and contaminated bedding shall be handled to minimize generation of dust and aerosols.  Radioactive bedding and cages will be held in “red bags” and labeled with “Caution Radioactive Material” tape and the isotope. Cage Change  Bedding and animal carcasses must be collected in “red bags” and disposed via EH&S as radioactive biological waste.  PI staff is responsible to ensure that animal cages are free of contamination (wipe tests performed) prior to transferring to IAS staff.  In the event of a radioactive spill, the area will be immediately restricted and cleaned and Decontamination surveyed by laboratory personnel. and Spill  Equipment and work surfaces must be routinely cleaned with 10% freshly prepared Procedure bleach or appropriate disinfectant. All equipment must be decontaminated prior to leaving the animal room. Animal monitoring/room checks performed by PI staff that has received specialized Monitoring instructions from IAS.

of 2 Animal checks daily (7 days per week) by: Animal Checks Name: Phone: After Hours Phone:

Holding Time Indicate specific holding time for animals below: (Must be provided) Additional Provide additional information not covered above here: Information Not Covered Above Please attach the hazard sign that will be affixed on the door where animals are to be Hazard Sign housed. The appropriate sign can be downloaded from the IACUC website