Comparative Studies of Three Novel Freshwater Microalgae Strains for Synthesis of Silver
Total Page：16
File Type：pdf, Size：1020Kb
Comparative studies of three novel freshwater microalgae strains for synthesis of
silver nanoparticles: Insights of characterization, antibacterial, cytotoxicity and
antiviral activities
Muneeba Khalid1, Nauman Khalid2,3, Iftikhar Ahmed4, Rumeza Hanif1, Muhammad Ismail5,
Hussnain Ahmed Janjua*1
1 Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Science and Technology (NUST), Islamabad, Pakistan
2 School of Food and Agricultural Sciences, University of Management and Technology, Lahore 54000, Pakistan
3Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Victoria, 3216, Australia
4 Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Centre (NARC), Park Road, Islamabad-45500, Pakistan
5 Institute of Biomedical and Genetic Engineering (IBGE), 24 Mauve Area, Sector G-9/1, Islamabad, Pakistan
------
Corresponding Author:
Hussnain A. Janjua; [email protected]; [email protected]; Tel: +
92 323 5680231, +92-51-9085-6140. Supplementary Figures
(a)
(b) (c)
Figure S1: Surface Plasmon Resonance bands for biologically synthesized AgNPs (a) DHM1-AgNPs spectrum showing sharp peak at 419nm (b) DHM2-AgNPs at 421nm (c) PHM3-AgNPs at 418nm. (a)
(b)
(c)
Figure S2: Scanning Electron Microscopy images depicting size and shape of AgNPs synthesized by ethanolic extracts of microalgae (a) DHM1-AgNPs (b) DHM2-AgNPs and (c) PHM3-AgNPs. (a)
Ag L = 76.2%
(b)
Ag L = 66.8 %
(c)
Ag L = 60.6 %
Figure S3: Energy Dispersive Spectroscopy images showing quantitative analysis of Ag in AgNPs (a) DHM1-AgNPs show 76.2% Ag (b) DHM2-AgNPs show 66.8% Ag (c) PHM3-AgNPs show 60.6% Ag. All three synthesized AgNPs showed presence of Ag in L shell of orbital. Figure S4: Antifungicidal activity of AgNPs against Candida albicans. ( ) fungal strain and ( ) control. Table S1:Antibacterial activity of synthesized AgNPs. The zone of inhibition (mm) variations with different shaped AgNPs. DHM1-AgNPs DHM2-AgNPs PHM3-AgNPs Bacterial Strains *IZ1 IZ2 IZ3 Avg. IZ IZ1 IZ2 IZ3 Avg. IZ IZ1 IZ2 IZ3 Avg. IZ E. coli 10 10 11 10 11 10 11 10.7 10 11 11 10.7 MRSA 8 13 11 10.7 9 13 12 11.4 10 13 14 12.4 S. typhi 12 14 10 12 10 13 13 12 11 15 15 13.7 Enterococcus 12 11 12 12 12 14 13 13 16 14 14 14.7 K. pneumoniae NA 8 10 9 NA 9 10 9.5 10 10 9 10 S.mutans 13 11 13 13 11 12 15 12.7 10 13 12 11.7 E.faecalis NA 9 9 9 NA 8 9 8.5 9 9 9 9 A.junii NA 16 14 15 NA 11 10 10.5 NA 15 14 14.5 S.aureus NA NA NA NA NA NA NA NA NA NA NA NA P. mirabilis 15 17 16 16 13 15 13 13.7 17 17 16 16.7 S.marcescens 10 15 12 12.4 13 13 10 12 15 15 12 14 Enterobacter 14 14 13 14 10 11 11 10.7 13 14 15 14 P.aeruginosa NA NA NA NA NA NA NA NA NA NA NA NA S.dysenteriae 14 10 12 13 10 9 9 9.4 13 10 10 11 *Zone of inhibition with replication 1, 2 and 3