BIOGRAPHICAL SKETCH Provide the following information for the Senior/key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Linda F. Hayward, PhD Associate Professor eRA COMMONS USER NAME (credential, e.g., agency login) lindah EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and residency training if applicable.) DEGREE INSTITUTION AND LOCATION MM/YY FIELD OF STUDY (if applicable) University of California, Santa Cruz, CA BA 12/1980 Psychobiology University of Washington, Seattle, WA MS 08/1984 Kinesiology Northwestern University, Chicago, IL PhD 06/1990 Physiology Cardiovascular University of Iowa, Iowa City, IA Postdoc 07/1997 neurophysiology

A. Personal Statement: I have a broad background in physiology and more focused training in central autonomic control and cardiovascular neurophysiology and neuropharmacology with 18 years of research experience. My research expertise is focused on investigating how activation of supramedullary regions in the brain modulates cardiorespiratory function/integration and neuronal activity/protein expression in targeted brain regions. Currently the three main projects are underway. The first is involved in evaluating the role of the hypothalamic neuropeptide orexin in mediating cardiorespiratory responses to physiological challenges during sleep versus wakefulness at different time points during postnatal development in an animal model of prenatal nicotine exposure. Second, we are evaluating the role of hypertension in modulating neural plasticity in response to repeated bouts of hypoxia. Third, projects involving the role of exercise in modulating cardiorespiratory function and neural plasticity are also underway. I also have a long-standing interest in sensory integration as it relates to the brainstem and the lateral parabrachial nucleus (LPBN), specifically, with a research focus on evaluating how this brain region is involved in both normal and dysregulation of the cardiorespiratory systems. The LPBN and many of the brain defense regions also play a role in nociception. The overall goal of a newly submitted proposal is to expand our knowledge of brain mechanisms involved in attenuating hyperalgesia. Current evidence suggests that visceral inputs associated with blood pressure regulation play an important role in nociception. In particular, evidence suggests that elevations in baroreceptor inputs can attenuate central processing of nociceptive inputs. The loss of appropriate baroreceptor afferent processing and associated autonomic dysfunction is often observed in individuals with chronic pain syndromes. Accordingly we hypothesize that central processing of nociceptive inputs can be indirectly modulated by treatments which improve baroreceptor afferent integration. One such therapy is exercise training. I have extensive experience with heart rate variability analysis, neural systems analysis, immunohistochemistry and central microinjection. I have successfully administered numerous projects previous, including NIH and American Heart grants and have produced peer-review publications from each project.

B. Positions and Honors: 1990-1994 Postdoctoral Associate, Dept. Internal Medicine, Univ. of Iowa, Iowa City, IA 1995-1997 Assistant Research Scientist, Dept. Internal Medicine, Univ. of Iowa, Iowa City, IA. 1997-2004 Assistant Professor, Dept. of Physiological Sciences, McKnight Brain Institute and the Dept. of Neuroscience (Affiliate appointment), Univ. of Florida, Gainesville, FL 2004-present Associate Professor, Dept. of Physiological Sciences, McKnight Brain Institute and Dept. of Neuroscience (Affiliate appointment), Univ. of Florida, Gainesville, FL. 2009-present Associate Chair, Dept. of Physiological Sciences, Univ. of Florida, Gainesville, FL Other experience and Profession Memberships 1992- Member American Physiological Society 1997- Member American Association for the Advancement of Science 1982-Member Society for Neuroscience 2000, 2003,2010 Grant Peer Reviewer, American Heart Association, Southeastern Research Consortium, Committee 3 June 2001 Ad Hoc Reviewer, NIH, NHLBI, Respiratory and Applied Physiology Study Section. 2002-2005 Member, American Heart Association, Florida/Puerto Rico Affiliate Research Committee Nov. 2003 Ad Hoc Reviewer, NIH, NHLBI, Cardiovascular and Renal Study Section. 2009-present Reviewer, NIH, NHLBI, F10A Physiology and Pathobiology of Cardiovascular and Respiratory Systems 2010 Ad hoc Reviewer, Louisiana State Board of Regents

Honors 1996-1997 Central Investment Research Enhancement Award Univ. of Iowa, Iowa City, IA 2001-2003 Who’s Who in America and Who’s Who in Science and Engineering June 2002 AHA-Robert J Boucek MD Research Award, Am. Heart Assoc. Stuart, Fl. April 2003 CE Cournelius Young Investigator Award, Univ. of Florida College of Vet. Med.

C. Most relevant to the current application 1. Porter, K, Ahlgren, J., Stanley, JT. and Hayward, LF, Modulation of heart rate variability during severe hemorrhage at different rats in conscious rats. Auton. Neurosci. 250:53-61, 2009. 2. Zhang, W., Hayward, LF., and Davenport, PW. Influence of dorsal periaqueductal gray on respiratory occlusion. Auton. Neurosci. 150: 62-69, 2009. 3. Fuller, DD., Dougherty, BJ., Sandhu, MS., Doperalski, NJ Reynolds, CR., Hayward., LF., Prenatal nicotine exposure alters respiratory long term facilitation in neonatal rats., Respiratory Physiology and Neurobiology, 169(3):333-7,. 2009. 4. Hayward, LF. Midbrain modulation of the cardiac baroreflex involves excitation of lateral parabrachial neurons in the rat. Brain Res., 1145: 117-127, 2007. 5. Reynolds, CR., Vujisic, K., Davenport, PW., and Hayward, LF. Disinhibition of dorsomedial hypothalamus increases the frequency of augmented breaths in the anesthetized rat. Adv. Exp. Med. Biol. 605: 274- 278, 2008.

Additional recent publications of importance to the field (in chronological order) 1. Hayward, LF., and Castellanos, M. Increased Fos expression in the lateral parabrachial nucleus following dorsal periaqueductal gray stimulation in rats. Brain Res. 974, 153-166, 2003. 2. Berry,RB. and Hayward, LF. Selective augmentation of genioglossus EMG activity by L-5- Hydroxytryptophan (L-5HTP) in the rat. Pharm.. Biochem and Behav., 74:877-882, 2003. 3. Hayward, LF, Castellanos, M., and Davenport, PW. Activation of parabrachial neurons mediates the cardiorespiratory response to dorsal periaqueductal gray stimulation J. Appl. Physiol. 96(3):1146-54, 2004. 4. Hayward, LF, and Castellanos, M. Activation of the dorsal periaqueductal gray induces Fos-like immunoreactivity in select non-cholinergic mesopontine neurons in the rat. Neurosci. Letters, 360 (1-2): 5- 8. 2004. 5. Berry, RB, Koch, G., and Hayward, LF. Low Dose Mirtazapine Increases Genioglossus Activity in the Anesthetized Rat. Sleep., 28: 78-84, 2005. 6. Zhang, W., Hayward, LF., Davenport, PW. Respiratory muscle responses elicited by dorsal periaqueductal gray stimulation in rats. Amer. J. Physiol.; 289(5):R1338-47. 2005. 7. Ahlgren, JK., Porter, K., and Hayward, LF. Hemodynamic responses and c-Fos changes associated with hypotensive hemorrhage. Amer. J. Physiol., 292: R1862-1871, 2007. 8. Hayward, LF. Midbrain modulation of the cardiac baroreflex involves excitation of lateral parabrachial neurons in the rat. Brain Res., 1145: 117-127, 2007. 9. Zhang, W., Hayward, LF., and Davenport, PW. Respiratory responses elicited by rostral versus caudal dorsal periaqueductal gray stimulation in rats. Auton. Neurosci., 134:45-54, 2007. 10. Chou, YL., Stanley, JT., Vujisic, K., Berry, RB., Davenport, PW., Anderson, JJ., and Hayward, LF. Effects of systemic administration of mirtazapine on respiratory muscle activity in sleeping rats. Adv. Exp. Med. Biol. 605: 257-261, 2008. 11. Reynolds, CR., Vujisic, K., Davenport, PW., and Hayward, LF. Disinhibition of dorsomedial hypothalamus increases the frequency of augmented breaths in the anesthetized rat. Adv. Exp. Med. Biol. 605: 274-278, 2008. 12. Hayward, LF. Current Advances in Autonomic Control During Sleep. In Recent Advances In Cardiovascular Research From Exercise to Sleep, Elsevier Publishing, p 1-21, 2010. 13. Porter, K., and Hayward, LF. Stress-induced changes heart rate and corticotropin releasing hormone immunoreactivity in the amygdala of the spontaneously hypertensive rat characterize autonomic hyperactivity. Behav. Brain Res., accepted upon revision, 2010. 14. Ahlgren, J., and Hayward, LF. Exercise induced plasticity in the autonomic response to severe hemorrhage in conscious male rats, Autonomic Neurosci., under review, 2010. 15. Reynolds, CR., Fuller, DD., and Hayward, LF. Impact of prenatal nicotine exposure and postnatal age on heart rate variability during sleep in conscious rat pups, under revision, 2010

Ongoing Extramural Research Support: None Ongoing Intramural Research Support: UF –CVM Research Development Award “The impact of acute stress on gene expression in the limbic system of hypertensive animals” PI: LF Hayward 6/1/2010-7/1/2012 Pending;

Exercise-induced neural plasticity in pain management 1/1/2011-12/31/2010-PIs LF Hayward, R Caudle

Prenatal modulation of respiratory plasticity, 4/1/2011-3/31/2016, PIs: LF Hayward, DD Fuller, CE Wood

. Complete Extramural Support in Last 2 Years: RO1 HL076518 Period 04/2004-03/31/2009 PI NIH/NHLBI “Supramedullary control of respiration” Major goal: To identify role of specific brainstem nuclei in mediating the cardiorespiratory response during fear and dorsal medial hypothalamic stimulation. 06-TSP-01 Period 07/2006-06/30/2008 Co-I Florida Dept. of Health James and Esther King Biomedical Research Program “The role of nicotine in the neural control of respiratory and cardiovascular systems” Major goal: To identify the impact of prenatal nicotine exposure on the cardiorespiratory response to hypoxia and hyperthermia in neonates (postnatal days 3 to 15).