The Central Dogma Dry Lab

Following is the base sequence of a gene on one strand of a DNA molecule (the SENSE STRAND):

A A T G C C A G T G G T T C G C A C

1. What is the sequence of the complementary DNA strand (i.e. the NONSENSE STRAND)?

2. What is the sequence of the mRNA transcribed from this gene?

3. Use the provided genetic code, to determine what amino acids will be in the protein coded for by this gene.

4. If the fourth nucleotide in the original DNA strand was changed from G to C, what sequence of mRNA would result? i.e., the DNA sequence is now A A T C C C A G T G G T T C G C A C

5. What sequence of amino acids would this code for?

6. If a G were added to the original DNA strand after the third nucleotide, what sequence of mRNA would result? i.e., the DNA sequence is now A A T G G C C A G T G G T T C G C A C

7. What sequence of amino acids would this code for?

8. If the eighth nucleotide in the original DNA strand was changed from a G to a C, what mRNA sequence would result? i.e., the DNA sequence is now A A T G C C A C T G G T T C G C A C

9. What sequence of amino acids would this code for? Why is this sequence so short? Second mRNA Base U C A G t s U phenylalanine Serine Tyrosine Cysteine U r i

F phenylalanine Serine Tyrosine Cysteine C A

N leucine Serine STOP STOP A R

m leucine Serine STOP tryptophan G e s a

C leucine proline Histidine arginine U T B h

leucine proline Histidine arginine C i r d

leucine proline Glutamine arginine A m

leucine proline Glutamine arginine G R N

A isoleucine threonine Aspragine serine U A

isoleucine threonine Asparagines serine C a s isoleucine threonine Lysine arginine A e Methionine (start) threonine Lysine arginine G G valine alanine Aspartic acid glycine U valine alanine Asprartic acid glycine C valine alanine Glutamic acid glycine A valine alanine Glutamic acid glycine G