Supplementary Fig. S1. Scanning Electron Microscope Image of Strain E4-1T

Supplementary Fig. S1. Scanning electron microscope image of strain E4-1T.

1 Supplementary Fig. S2. Maximum-likelihood tree based on 16S rRNA gene sequences of strain E4-1T and related taxa. Numbers at nodes are bootstrap percentages based on 1000 replicated datasets. DDBJ/EMBL/GenBank accession numbers are shown in parentheses. Bar, 0.02 substitutions per nucleotide position. The type species of the corresponding genera are indicated by asterisks (*). Xanthobacter autotrophicus 7cT was used as an outgroup.

2 Supplementary Fig. S3. Maximum-parsimony tree based on 16S rRNA gene sequences of strain E4-1T and related taxa. Bootstrap values are shown in percentages of 1000 replicates, when greater than 50%. Bar, 20 substitutions per nucleotide position. The type species of the corresponding genera are indicated by asterisks (*). Xanthobacter autotrophicus 7cT was used as an outgroup.

3 Supplementary Fig. S4. Two-dimensional TLC separation of total polar lipids from strain strain E4-1T and closely related strains after staining with 10 % molybdatophosphoric acid. (a) strain E4-1T; (b) Chiayiivirga flava DSM 24163T

4 Supplementary Fig. S5. Identification of polyamines by TLC. Dansyl polyamine derivatives of cell extracts were analyzed by TLC in ethyl acetate-cyclohexane (2:3, vol/vol). Lane 1, standard; lane 2, strain E4-1T

5 Supplementary Table S1. Cellular fatty acid composition (%) of strain E4-1T and the type strains of related taxa.

Strains: 1, E4-1 T; 2, Chiayiivirga flava DSM 24163T; 3, Aquimonas voraii DSM 16957T; 4, Dokdonella koreensis DS-123T (Yoon et al., 2006); 5, Frateuria aurantia LMG 1558T (Swings et al., 1980); 6, Rhodanobacter lindaniclasticus RP5557T (Nalin et al., 1999 and Woo et al., 2012); 7, Dyella japonica XD53T (Xie & Yokota, 2005). tr, trace (<1%); -, not detected.

Fatty acid 1 2 3 4 5 6 7

Saturated acid

C10:0 tr tr tr - - - -

C11:0 - - tr - - - -

C12:0 - tr tr - - - -

C14:0 1 tr 1.5 tr 1.2 - tr

C15:0 2.2 - tr - - - -

C16:0 2.5 2.4 5.9 4.6 4.8 6·3 1·5

C17:0 - - tr - - - -

C18:0 - - tr tr - - tr

Unsaturated acid

C14:1 ω5c - tr - - - - -

C16:1 ω5c - - tr - - - -

C16:1 ω7c - - tr - - - -

C16:1 ω9c - - tr - - - -

C16:1 ω11c - tr - - - - -

C17:1 ω6c - - - tr 3.4 - -

C18:1 ω5c - - tr - - - -

C18:1 ω7c - - tr - - - -

C18:1 ω9c - - tr - - - -

Branched acid

iso-C10:0 - 1.2 tr - - - -

6 iso-C11:0 11.6 24.9 18.4 4.7 3.7 3·5 3·8

iso-C12:0 - 1.7 2.6 - - - -

iso-C13:0 tr tr tr tr - - tr

iso-C14:0 tr 4.7 1.1 - 1.8 - tr

iso-C15:0 35.8 13.4 12.9 10.1 21.3 21·3 23·6

iso-C16:0 1.3 10.1 10.7 6.2 - 2·2 4·9

iso-C17:0 tr 1.1 1.5 19.8 4.2 10·9 20·0

iso-C18:0 - - tr - - - -

iso-C19:0 ------0·7

iso-C14:1 E - tr tr - - - -

iso-C15:1 - - - tr - 1·2 -

iso-C15:1 ω5c 7.3 ------

iso-C15:1 F - tr 1.8 - - - -

iso-C16:1 H - tr tr - - - -

iso-C18:1 H - - tr - - - -

iso-C17:1 ω9c 16.9 2.8 10.6 31.2 19.5 24·6 25·6

anteiso-C11:0 tr tr tr - - - -

anteiso-C13:0 tr tr tr - - - -

anteiso-C15:0 4.6 2.2 tr 3.7 - 6·7 1·6

anteiso-C17:0 - tr tr 7.7 - - 0·7

Hydroxyl

C10:0 3OH tr tr tr - - - -

C11:0 2OH tr tr tr - - - -

C12:0 3OH - tr tr - - - -

iso-C11:0 3OH 11.5 16.4 20.0 6.8 9.1 4.4 3.7

iso-C12:0 3OH - 1.4 tr tr 2.4 - tr

iso-C13:0 3OH - - - - 3.7 3 3.9

iso-C15:0 3OH tr ------

7 iso-C16:0 3OH - - tr - - - -

iso-C17:0 3OH - tr tr - - - 1.1

alchol C16:1 ω7c - 2.2 tr - - - -

10 methyl C17:0 - - tr - - - -

cyclo C17:0 - tr - - tr 2 -

Summed feature 1 - tr tr - - - -

Summed feature 3 1.3 7.5 2.2 1 - 5.9 4

Summed feature 4 - - tr - - - -

ECL 11.799 - tr - tr 5.7 2.2 1.9

* Summed features represent groups of two or three fatty acids that could not be separated by GLC using the MIDI system. Summed feature 1 contained iso-C15 : 1 H and/or C13 : 0 3OH; summed feature 3 contained C16 : 1ω7c and/or C16 : 1ω6c; summed feature 4 contained iso-C17 : 1

I and/or anteiso-C17 : 1 B.