Videos 1-3, 7-12: GFP-Histone H2B & GFP-G-Tubulin

Desai et al. Supplemental Material

Video Info

NOTE: In all videos, the anterior of the embryo is to the left (or lower left) and the posterior of the embryo is to the right (or upper right).

Details:

Videos 1-3, 7-12: GFP-histone H2B & GFP--tubulin (widefield deconvolution microscopy to analyze chromosome segregation and spindle pole separation) i) One 3D z-stack (6 images at 2 µm steps) acquired every 10s ii) Maximal intensity projections of z-stacks used to generate each movie frame iii) Video playback is at 10 frames/sec

Video 1: Wild-type embryo Video 2: KNL-1 depleted embryo Video 3: CeCENP-C depleted embryo

Video 7: Wild-type embryo Video 8: KNL-1 depleted embryo Video 9: CeNDC-80 depleted embryo #1 Video 10: CeNDC-80 depleted embryo #2 Video 11: HIM-10 depleted embryo #1 Video 12: HIM-10 depleted embryo #2

Videos 4-6, 13-14: GFP--tubulin (spinning disk confocal imaging to analyze spindle morphology) i) One confocal section acquired every 8 s (except every 6 s for Video 6) ii) Video playback is at 10 frames/sec

Video 4: Wild-type embryo Video 5: KNL-1 depleted embryo Video 6: CeCENP-C depleted embryo

Video 13: CeNDC-80 depleted embryo Video 14: HIM-10 depleted embryo