BIO 105 Lab 2 Mitosis, Meiosis and Gametogenesis Bgee 130201.1

Biology 105 – Human Biology Session: Spring 2015 Section: 55244 / 61816 4 Units Class Location: UVC1, 3 and 7 St. Helena Days / Time: F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11;50 AM LAB 61816 Instructor: RIDDELL Laboratory Report

Student Author Name: Team Members Name(s): Student Author ID #: Team Members ID #’(s): Lab Assignment #: Team Name: Lab Assignment Title: Date:

Purpose / Objective(s):

. Observe Cheek Cells . Observe Human Gametogenesis in prepared MS Slides via standard Light Microscopy . Survey and capture illustrations and photos from the internet of the above . Measure Cell and Structure Size utilizing Field of View Calculator

Hypothesis: NA

Materials / Subjects / Specimens: Specimen of cheek cells form self Prepared MS slides featuring a cross-section of: . Sperm Smear Human . . Ovary Maturing, Follicle Human . . Methods / Tools / Instrumentation / Procedures: 1. Prepared a MS sample of cheek cells from self and observed via standard Light Microscopy 2. Standard Light microscope with 100, 400 and 1000 Magnification 3. View cheek cells at 100X and 400 X 4. View Human sperm smear, and view ovary maturing, and follicle sections in human ovary

0aeeac06e85acc4506744ff5e93ffb46.doc Biology 105 – Human Biology Session: Spring 2015 Section: 55244 / 61816 4 Units Class Location: UVC1, 3 and 7 St. Helena Days / Time: F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11;50 AM LAB 61816 Instructor: RIDDELL Laboratory Report

Results . Viewing the target specimens and structures with the standard light microscopes required skill and patience.

. Gametogenesis tissues and cells are presented in Table 1 o Male and Female structures . Sample measurements from the FOV Calculator are presented in Table 2 . Table 3 lists the size measurements for the cells and structures of Gametogenesis . Figure 1 compares the cells and structure sizes of Gametogenesis

Analysis / Discussion: . . … . …. . …. . ….. Research

Events of Meiosis

Interphase Cell growth, cell doubling DNA. Chromosomes duplicate Chromatin inside nuclear envelope, and centriole pair with another centriole to make centrioles

Prophase Centromere attaches to the spindles and the centromere begin to form. Spindles stretch and spread apart and the centromere begin to bind to the spindles. In the process of synapsis the two sister chromatids form and attach together

Metaphase The chromosome tetrads are aligned on the plate. The centrioles are at opposite sides of the pole. Spindle fibers are aligned around the entire cell.

Anaphase Daughter chromosomes begin to split apart and leave to opposite ends of the cell; the spindles also start to break apart. The centrioled remain at opposite ends of the poles.

Telophase Cleavage furrow is in between the cells and the two cells are beginning to form a formation to leave into the next stage of Cytokinesis. This is when the cell officially forms into two cells and the two sister chromatids separate. Then the cycle repeats itself into mitosis, and in the very end we are left with four duplicate cells.

Conclusions/Further Considerations:

. ……… . ……….. . ………….

References 1. http://webnt.calhoun.edu/distance/internet/natural/anatomy/EndoRepro/ovary4X.html

2. http://faculty.baruch.cuny.edu/jwahlert/bio1003/mitosis.html

ATTACHMENTS:

Table 1: Illustrations and Micrographs of Each Mitosis Stage

Stage Key Event(s) Notes / Illustration/ Photo Description / Slide Diagram Micrograph Specimen Interphase Chromosomes Cell growth begin to form

Prophase Chromatids pair Replicated up and make chromosomes, chromosomes consisting of two sister chromatids held together at the centromere Metaphase Chromosomes chromosomes shre DNA and assemble on the line up in a line equatorial plate

Anaphase Chromosomes Sister chromatids split apart separate from each other and move to opposite poles of the cell.

Telophase All DNA is Cells are separated & doubles and split Cytoknesis into their cells

Web Reference for white fish and Onion Root cells: http://faculty.baruch.cuny.edu/jwahlert/bio1003/mitosis.html

Table 2: Illustration and Photo Micrographs of Meiosis 1 and Meiosis 2

Stages Meiosis Key event(s) Notes/Description/ Illustration Photo-Micrograph slide specimen Meiosis II Meiosis II Prophase Phase 1 Cleavage Cells uncoil and furrow is nuclear envelope present in forms between both cells

Metaphase Phase 2 Spindles align Chromosomes pair with each other up with each other in a straight line

anaphase Phase 3 Spindles Sister chromatids spread apart separate in and anaphase two, as for chromosomes anaphase one where split they stay together

Telophase 2 Phase 4 Cleavage After it forms then it furrow begins separates and forms to form around the cell.

Table 3: Illustrations/Notes of cells- (Human Gametogenesis , Testis, Sperm, and Ovary

Table 3 Gametogenesis and Human Development

http://webnt.calhoun.edu/distance/internet/natural/anatomy/EndoRepro/ovary4X.html

0aeeac06e85acc4506744ff5e93ffb46.doc