<p> Biology 105 – Human Biology Session: Spring 2015 Section: 55244 / 61816 4 Units Class Location: UVC1, 3 and 7 St. Helena Days / Time: F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11;50 AM LAB 61816 Instructor: RIDDELL Laboratory Report</p><p>Student Author Name: Team Members Name(s): Student Author ID #: Team Members ID #’(s): Lab Assignment #: Team Name: Lab Assignment Title: Date:</p><p>Purpose / Objective(s): </p><p>. Observe Cheek Cells . Observe Human Gametogenesis in prepared MS Slides via standard Light Microscopy . Survey and capture illustrations and photos from the internet of the above . Measure Cell and Structure Size utilizing Field of View Calculator</p><p>Hypothesis: NA</p><p>Materials / Subjects / Specimens: Specimen of cheek cells form self Prepared MS slides featuring a cross-section of: . Sperm Smear Human . . Ovary Maturing, Follicle Human . . Methods / Tools / Instrumentation / Procedures: 1. Prepared a MS sample of cheek cells from self and observed via standard Light Microscopy 2. Standard Light microscope with 100, 400 and 1000 Magnification 3. View cheek cells at 100X and 400 X 4. View Human sperm smear, and view ovary maturing, and follicle sections in human ovary</p><p>0aeeac06e85acc4506744ff5e93ffb46.doc Biology 105 – Human Biology Session: Spring 2015 Section: 55244 / 61816 4 Units Class Location: UVC1, 3 and 7 St. Helena Days / Time: F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11;50 AM LAB 61816 Instructor: RIDDELL Laboratory Report</p><p>Results . Viewing the target specimens and structures with the standard light microscopes required skill and patience.</p><p>. Gametogenesis tissues and cells are presented in Table 1 o Male and Female structures . Sample measurements from the FOV Calculator are presented in Table 2 . Table 3 lists the size measurements for the cells and structures of Gametogenesis . Figure 1 compares the cells and structure sizes of Gametogenesis</p><p>Analysis / Discussion: . . … . …. . …. . ….. Research</p><p>Events of Meiosis</p><p>Interphase Cell growth, cell doubling DNA. Chromosomes duplicate Chromatin inside nuclear envelope, and centriole pair with another centriole to make centrioles</p><p>Prophase Centromere attaches to the spindles and the centromere begin to form. Spindles stretch and spread apart and the centromere begin to bind to the spindles. In the process of synapsis the two sister chromatids form and attach together</p><p>Metaphase The chromosome tetrads are aligned on the plate. The centrioles are at opposite sides of the pole. Spindle fibers are aligned around the entire cell.</p><p>Anaphase Daughter chromosomes begin to split apart and leave to opposite ends of the cell; the spindles also start to break apart. The centrioled remain at opposite ends of the poles.</p><p>Telophase Cleavage furrow is in between the cells and the two cells are beginning to form a formation to leave into the next stage of Cytokinesis. This is when the cell officially forms into two cells and the two sister chromatids separate. Then the cycle repeats itself into mitosis, and in the very end we are left with four duplicate cells.</p><p>0aeeac06e85acc4506744ff5e93ffb46.doc Biology 105 – Human Biology Session: Spring 2015 Section: 55244 / 61816 4 Units Class Location: UVC1, 3 and 7 St. Helena Days / Time: F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11;50 AM LAB 61816 Instructor: RIDDELL Laboratory Report</p><p>.</p><p>0aeeac06e85acc4506744ff5e93ffb46.doc Biology 105 – Human Biology Session: Spring 2015 Section: 55244 / 61816 4 Units Class Location: UVC1, 3 and 7 St. Helena Days / Time: F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11;50 AM LAB 61816 Instructor: RIDDELL Laboratory Report</p><p>Conclusions/Further Considerations: </p><p>. ……… . ……….. . ………….</p><p>References 1. http://webnt.calhoun.edu/distance/internet/natural/anatomy/EndoRepro/ovary4X.html</p><p>2. http://faculty.baruch.cuny.edu/jwahlert/bio1003/mitosis.html</p><p>0aeeac06e85acc4506744ff5e93ffb46.doc Biology 105 – Human Biology Session: Spring 2015 Section: 55244 / 61816 4 Units Class Location: UVC1, 3 and 7 St. Helena Days / Time: F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11;50 AM LAB 61816 Instructor: RIDDELL Laboratory Report</p><p>ATTACHMENTS:</p><p>Table 1: Illustrations and Micrographs of Each Mitosis Stage</p><p>Stage Key Event(s) Notes / Illustration/ Photo Description / Slide Diagram Micrograph Specimen Interphase Chromosomes Cell growth begin to form</p><p>Prophase Chromatids pair Replicated up and make chromosomes, chromosomes consisting of two sister chromatids held together at the centromere Metaphase Chromosomes chromosomes shre DNA and assemble on the line up in a line equatorial plate</p><p>Anaphase Chromosomes Sister chromatids split apart separate from each other and move to opposite poles of the cell.</p><p>Telophase All DNA is Cells are separated & doubles and split Cytoknesis into their cells</p><p>Web Reference for white fish and Onion Root cells: http://faculty.baruch.cuny.edu/jwahlert/bio1003/mitosis.html </p><p>0aeeac06e85acc4506744ff5e93ffb46.doc Biology 105 – Human Biology Session: Spring 2015 Section: 55244 / 61816 4 Units Class Location: UVC1, 3 and 7 St. Helena Days / Time: F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11;50 AM LAB 61816 Instructor: RIDDELL Laboratory Report</p><p>Table 2: Illustration and Photo Micrographs of Meiosis 1 and Meiosis 2</p><p>Stages Meiosis Key event(s) Notes/Description/ Illustration Photo-Micrograph slide specimen Meiosis II Meiosis II Prophase Phase 1 Cleavage Cells uncoil and furrow is nuclear envelope present in forms between both cells</p><p>Metaphase Phase 2 Spindles align Chromosomes pair with each other up with each other in a straight line</p><p> anaphase Phase 3 Spindles Sister chromatids spread apart separate in and anaphase two, as for chromosomes anaphase one where split they stay together</p><p>Telophase 2 Phase 4 Cleavage After it forms then it furrow begins separates and forms to form around the cell.</p><p>0aeeac06e85acc4506744ff5e93ffb46.doc Biology 105 – Human Biology Session: Spring 2015 Section: 55244 / 61816 4 Units Class Location: UVC1, 3 and 7 St. Helena Days / Time: F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11;50 AM LAB 61816 Instructor: RIDDELL Laboratory Report</p><p>Table 3: Illustrations/Notes of cells- (Human Gametogenesis , Testis, Sperm, and Ovary</p><p>Table 3 Gametogenesis and Human Development</p><p> http://webnt.calhoun.edu/distance/internet/natural/anatomy/EndoRepro/ovary4X.html</p><p>0aeeac06e85acc4506744ff5e93ffb46.doc </p>