1Supplementary material

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4Fig. S1 Quantitative PCR plots showing the exponential stage of the ITS2A+B amplicon 5(left bundle of curves) starts at about cycle no. 15, whereas that of the ITS2 B amplicon 6does at about cycle no. 30

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1 1 8 Table S1 Multiple post-hoc comparisons, by means of Tukey contrasts, for RQ values of the ITS2A+B amplicon between the six body parts. Z and P values are placed above and below diagonal, respectively. H= Head, HL= Hind leg, WM= Wing muscle, T= Testis, AG= Accessory gland, GC= Gastric caecum. Significant P values are in bold-type letter.

H GC AG WM HL T H 0.473 5.062 5.766 0.459 4.922 GC 0.997 4.749 5.478 0.015 4.604 AG <0.0001 <0.0001 0.729 4.764 0.145 WM <0.0001 <0.0001 0.978 5.493 0.873 HL 0.997 1 <0.0001 <0.0001 4.619 T <0.0001 <0.0001 1 0.953 <0.0001

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11 Table S2 Multiple post-hoc comparisons, by means of Tukey contrasts, for RQ values of the ITS2B amplicon between the six body parts. Z and P values are placed above and below diagonal, respectively. H= Head, HL= Hind leg, WM= Wing muscle, T= Testis, AG= Accessory gland, GC= Gastric caecum. Significant P values are in bold-type letter.

H GC AG WM HL T H 0.141 2.122 2.27 0.275 2.427 GC 1 2.044 2.198 0.138 2.359 AG 0.276 0.317 0.153 1.906 0.315 WM 0.206 0.239 1 2.059 0.162 HL 1 1 0.398 0.309 2.221 T 0.147 0.171 1 1 0.228 12

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