Product datasheet [email protected]
ARG54887 Package: 100 μl anti-KMT5A / SET07 antibody Store at: -20°C
Summary
Product Description Mouse Monoclonal antibody recognizes KMT5A / SET07
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 43AT551.86.76
Isotype IgG1
Target Name KMT5A / SET07
Immunogen SET07 recombinant protein.
Conjugation Un-conjugated
Alternate Names Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; SET07; SET domain- containing protein 8; N-lysine methyltransferase SETD8; KMT5A; PR/SET domain-containing protein 07; PR-Set7; H4-K20-HMTase SETD8; EC 2.1.1.43; EC 2.1.1.-; PR/SET07; SET8
Application Instructions
Application table Application Dilution
ICC/IF 1:25
IHC-P Assay-dependent
WB 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Properties
Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.
www.arigobio.com 1/3 Bioinformation
Database links GeneID: 387893 Human
Swiss-port # Q9NQR1 Human
Gene Symbol SETD8
Gene Full Name SET domain containing (lysine methyltransferase) 8
Function Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. [UniProt]
Resrarch Area Gene Regulation antibody
Cellular Localization Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress- dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin
Images
ARG54887 anti-KMT5A / SET07 antibody WB image
Western blot: 35 μg of 293, 293T cell line (from left to right) stained with ARG54887 anti-KMT5A / SET07 antibody at 1:1000 dilution.
ARG54887 anti-KMT5A / SET07 antibody ICC/IF image
Immunofluorescence: MCF-7 cells stained with ARG54887 anti- KMT5A / SET07 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
www.arigobio.com 2/3 ARG54887 anti-KMT5A / SET07 antibody IHC image
Immunohistochemistry: paraffin-embedded H.breast carcinoma section stained with ARG54887 anti-KMT5A / SET07 antibody at 1:25 dilution.
ARG54887 anti-KMT5A / SET07 antibody IHC image
Immunohistochemistry: paraffin-embedded H. testis section stained with ARG54887 anti-KMT5A / SET07 antibody at 1:25 dilution.
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