Product datasheet [email protected]

ARG54887 Package: 100 μl anti-KMT5A / SET07 antibody Store at: -20°C

Summary

Product Description Mouse Monoclonal antibody recognizes KMT5A / SET07

Tested Reactivity Hu

Tested Application ICC/IF, IHC-P, WB

Host Mouse

Clonality Monoclonal

Clone 43AT551.86.76

Isotype IgG1

Target Name KMT5A / SET07

Immunogen SET07 recombinant protein.

Conjugation Un-conjugated

Alternate Names Histone- N-methyltransferase SETD8; Lysine N-methyltransferase 5A; SET07; SET domain- containing protein 8; N-lysine methyltransferase SETD8; KMT5A; PR/SET domain-containing protein 07; PR-Set7; H4-K20-HMTase SETD8; EC 2.1.1.43; EC 2.1.1.-; PR/SET07; SET8

Application Instructions

Application table Application Dilution

ICC/IF 1:25

IHC-P Assay-dependent

WB 1:1000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid

Purification Purification with Protein G.

Buffer PBS and 0.09% (W/V) Sodium azide

Preservative 0.09% (W/V) Sodium azide

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

www.arigobio.com 1/3 Bioinformation

Database links GeneID: 387893 Human

Swiss-port # Q9NQR1 Human

Gene Symbol SETD8

Gene Full Name SET domain containing (lysine methyltransferase) 8

Function Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic . Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. [UniProt]

Resrarch Area Gene Regulation antibody

Cellular Localization Nucleus. Chromosome. Note=Specifically localizes to mitotic . Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress- dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin

Images

ARG54887 anti-KMT5A / SET07 antibody WB image

Western blot: 35 μg of 293, 293T cell line (from left to right) stained with ARG54887 anti-KMT5A / SET07 antibody at 1:1000 dilution.

ARG54887 anti-KMT5A / SET07 antibody ICC/IF image

Immunofluorescence: MCF-7 cells stained with ARG54887 anti- KMT5A / SET07 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

www.arigobio.com 2/3 ARG54887 anti-KMT5A / SET07 antibody IHC image

Immunohistochemistry: paraffin-embedded H.breast carcinoma section stained with ARG54887 anti-KMT5A / SET07 antibody at 1:25 dilution.

ARG54887 anti-KMT5A / SET07 antibody IHC image

Immunohistochemistry: paraffin-embedded H. testis section stained with ARG54887 anti-KMT5A / SET07 antibody at 1:25 dilution.

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