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GeneTex, Inc : Toll Free 1-877-GeneTex (1-877-436-3839) Fax:1-949-309-2888 [email protected] GeneTex International Corporation : Tel:886-3-6208988 Fax:886-3-6208989 [email protected] Date : 2018/08/02 GTX118109 Package:25 ul,100 ul Catalog Number ( 1 ) Product Name EIF4G3 antibody [N1], N-term Full Name eukaryotic 4 gamma, 3 eIF-4G 3 antibody, eIF4G 3 antibody, eIF4GII antibody, EIF4G3 antibody, eIF-4-gamma 3 Synonyms antibody, initiation factor 4 gamma 3 antibody, eIF-4-gamma II antibody, "eukaryotic translation initiation factor 4 gamma, 3 antibody" Host Rabbit Clonality Polyclonal Isotype IgG Recombinant encompassing a sequence within the center region of human EIF4G3. The Immunogen exact sequence is proprietary. Antigen Species Human Species Reactivity Human, Mouse, Rat Applications ICC/IF, IHC-Fr, IHC-P, IP, WB Suggested dilution Reference Immunocytochemistry/ Immunofluorescence (ICC/IF) 1:100-1:1000* IHC (Frozen sections) 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Application Note Immunoprecipitation 1:100-1:500* Western Blot (WB) 1:500-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher. Positive Controls 293T , A431 , HeLa , HepG2 , mouse testis Predicted Target Size 177 kDa Conjugation Unconjugated Form Supplied Liquid Purification Purified by antigen-affinity chromatography. Concentration 0.26 mg/ml (Please refer to the vial label for the specific concentration) Storage Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ﾂｺC or below. Avoid multiple freeze-thaw Storage Instruction cycles. For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or Notes animals. Not for animal or human consumption. ResearchArea Metabolism > RNA metabolism Application Reference 1. Sundararaman B (2016) Mol Cell 903-13

GTX118109.html[2018/08/08 12:42:31] GTX118109 IHC-P Image EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. EIF4G3 antibody [N1], N-term (GTX118109) dilution: 1:500.

GTX118109 IHC-Fr Image EIF4G3 antibody [N1], N-term detects EIF4G3 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).

GTX118109.html[2018/08/08 12:42:31] GTX118109 IHC-P Image EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. EIF4G3 antibody [N1], N-term (GTX118109) dilution: 1:500.

GTX118109 IHC-P Image EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. EIF4G3 antibody [N1], N-term (GTX118109) dilution: 1:500.

GTX118109.html[2018/08/08 12:42:31] GTX118109 ICC/IF Image EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

GTX118109 IP Image Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 ug of EIF4G3 antibody [N1], N-term (GTX118109). Western blot analysis was performed using EIF4G3 antibody [N1], N-term (GTX118109). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX118109.html[2018/08/08 12:42:31] GTX118109 WB Image Mouse tissue extract (50 ﾂｵg) was separated by 5% SDS-PAGE, and the membrane was blotted with EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX118109 WB Image Various whole cell extracts (30 ﾂｵg) were separated by 5% SDS-PAGE, and the membrane was blotted with EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:10000.

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