Anti-TTBK1 Monoclonal Antibody, Clone G398-2.2-2F0

Anti-TTBK1 monoclonal antibody, clone G398-2.2- 2F0 (CABT-B1463) This product is for research use only and is not intended for diagnostic use.

PRODUCT INFORMATION

Specificity Expected to react with both spliced isoforms reported by UniProt (Q5TCY1-1 and Q5TCY1-2). It detected the full-length as well as the proteolytically processed human and mouse TTBK1.

Immunogen His-tagged recombinant fragment within the kinase domain of human TTBK1.

Isotype IgG2a, κ

Source/Host Mouse

Species Reactivity Mouse, Human

Clone G398-2.2-2F0

Purification Protein G Purified

Conjugate Unconjugated

Applications WB, IHC

Epitope Kinase domain.

Molecular Weight ~142 observed. 142.7 kDa (isoform 1) and 69.08 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Format Liquid

Concentration Please refer to lot specific datasheet.

Size 100 μg

Buffer 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preservative 0.05% Sodium Azide

Storage Stable for 1 year at 2-8°C from date of receipt.

BACKGROUND

Introduction Tau-tubulin kinase 1 (EC 2.7.11.1; UniProt Q5TCY1; also known as Brain-derived tau kinase) is

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]

Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved encoded by the TTBK1 (also known as BDTK, KIAA1855) gene (Gene ID 84630) in human. Tau- tubulin kinase 1 and 2 (TTBK1 and TTBK2) belong to the casein kinase 1 superfamily of kinases. TTBK2 is ubiquitously expressed, while TTBK1 is neuron-specific and can directly phosphorylate tau proteins at multiple Ser/Thr residues that are found on paired helical filament-tau or PHF-tau in Alzheimers disease (AD) brains. Consistently, transgenic mice over-expressiong human TTBK1 develop age-dependent pathology in brain, including intraneuronal accumulation of phosphorylated neurofilament (p-NF), tau phosphorylation, cortical and hippocampal astro/microgliosis, calpain I up-regulation, enhanced CDK5/p35 complex formation, down regulation of NMDA receptor, and memory impairment.

GENE INFORMATION

Entrez Gene ID 84630

UniProt ID Q5TCY1

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]